A5089211070- Vagus Nerve Stimulation Research
- Nicotinic Acetylcholine Receptors Study
- Cancer Research and Treatments
- Enhanced Recovery After Surgery
- Ion Channels and Receptors
- Gastrointestinal motility and disorders
- Nanoplatforms for cancer theranostics
- Neonatal Respiratory Health Research
- Clinical Nutrition and Gastroenterology
- Congenital Diaphragmatic Hernia Studies
- Immune Cell Function and Interaction
- Amoebic Infections and Treatments
- Respiratory and Cough-Related Research
- Pediatric Pain Management Techniques
- Immune cells in cancer
- Anesthesia and Neurotoxicity Research
- Regulation of Appetite and Obesity
- Intensive Care Unit Cognitive Disorders
- Infant Health and Development
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Fibroblast Growth Factor Research
KU Leuven
2015-2021
Objectives Vagus nerve stimulation (VNS), most likely via enteric neurons, prevents postoperative ileus (POI) by reducing activation of alpha7 nicotinic receptor (α7nAChR) positive muscularis macrophages (mMφ) and dampening surgery-induced intestinal inflammation. Here, we evaluated if 5-HT4 (5-HT4R) agonist prucalopride can mimic this effect in mice human. Design Using Ca 2+ imaging, the electrical field (EFS) was situ on mMφ evoked ATP jejunal tissue. Next, preoperative administration (1–5...
Objective Postoperative ileus (POI) is assumed to result from myeloid cells infiltrating the intestinal muscularis externa (ME) in patients undergoing abdominal surgery. In current study, we investigated role of monocytes a murine model manipulation (IM)-induced POI order clarify whether mediate tissue damage and dysfunction or they are rather involved recovery gastrointestinal (GI) motility. Design IM was performed mice with defective monocyte migration tissues (C-C motif chemokine receptor...
Oxazolone-induced colitis has been frequently used in literature as a model of IBD, but insights into the underlying immune response and pathological features are surprisingly still very limited. Vagus nerve stimulation (VNS) proven to be effective innate Th1/Th17 predominant inflammatory models, including pre-clinical models colitis, however what extent VNS is also ameliorating Th2-driven remains studied. In present study, we therefore further characterized oxazolone-induced investigated...
Vagotomy (VGX) increases the susceptibility to develop colitis suggesting a crucial role for cholinergic anti-inflammatory pathway in regulation of immune responses. Since oral tolerance and generation regulatory T cells (Tregs) are preserve mucosal homeostasis, we studied effect vagotomy involvement α7 nicotinic receptors (α7nAChR) at steady state during colitis. Therefore, development both (induced by dextran sulfate sodium (DSS) or via cell transfer) was vagotomized mice α7nAChR−/− mice....
Background: We previously showed increased susceptibility to dextran sulfate sodium (DSS)-induced colitis in vagotomized mice. Here, we evaluated whether vagus nerve stimulation (VNS) is able reduce the severity of DSS and aimed unravel mechanism involved. Methods: Colitis was induced wild type mice by 2.5% administration drinking water for 5 days. VNS (5 Hz, 1 ms, mA) applied day prior after 4 days evaluate changes epithelial integrity inflammatory response, respectively. Epithelial...
Ulcerative colitis [UC] is associated with excessive neutrophil infiltration and collateral tissue damage, but the link not yet completely understood. Since c-MET receptor tyrosine kinase [MET] required for chemoattraction cytotoxicity in response to its ligand hepatocyte growth factor [HGF], we aimed identify function of HGF-MET signalling neutrophils UC patients mice during intestinal inflammation. Serum colonic biopsies from healthy controls active [Mayo endoscopic subscore 2-3] inactive...
The orexigenic peptide ghrelin has anti-inflammatory properties in colitis, however, the mechanism of action and immune cells targeted remain still to be elucidated. Here, we assessed possible effect on T helper (Th) a cell transfer model chronic colitis.Disease was induced recombination activating gene 1 knockout mice (Rag1(-/-) ) by adoptive naïve Th from receptor (GRLN-R(-/-) or littermate wild-type (WT) mice. course severity colitis monitoring body weight, diarrhea score, histological...
Background: During intestinal inflammation, neutrophils aid in the recruitment of other immune cells and facilitate response gut. However, during chronic inflammatory conditions, such as Inflammatory Bowel Disease (IBD, excessive neutrophil accumulation can lead to tissue damage, delayed repair loss homeostasis. Thus, we aim identify role function pathogenesis colitis. Methods: To block chemoattraction cytotoxicity its ligand hepatocyte growth factor, used neutrophil-specific Mrp8-Cre line...