A5080014856- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Allelopathy and phytotoxic interactions
- Natural product bioactivities and synthesis
- Analytical Chemistry and Chromatography
- Antioxidant Activity and Oxidative Stress
- Chemical synthesis and alkaloids
- Plant biochemistry and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Electrochemical Analysis and Applications
- Tryptophan and brain disorders
- Plant-derived Lignans Synthesis and Bioactivity
- Fermentation and Sensory Analysis
- Plant tissue culture and regeneration
- Biochemical and biochemical processes
- Food Quality and Safety Studies
- Mass Spectrometry Techniques and Applications
- Gut microbiota and health
- GABA and Rice Research
- Free Radicals and Antioxidants
- Microbial Natural Products and Biosynthesis
- Biochemical Acid Research Studies
- Herbal Medicine Research Studies
- Proteins in Food Systems
- Plant Micronutrient Interactions and Effects
Wageningen University & Research
2015-2025
Food & Nutrition
2013
Interaction of tea phenolics with gut microbiota may play an integral role in the health benefits these bioactive compounds, yet this interaction is not fully understood. Here, metabolic fate epigallocatechin-3-gallate (EGCG) and its impact on were integrally investigated via vitro fermentation. As revealed by ultrahigh performance liquid chromatography hybrid quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS), EGCG was promptly degraded into a series metabolites, including...
Old tea leaves (OTL), which are normally discarded as agricultural waste, potential sources of phenolic compounds. However, little details known about their composition. In this study, the profiles OTL and young (YTL) were compared by using ultra-high performance liquid chromatography coupled to hybrid quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS) analyses, with an untargeted approach. A total 60 compounds tentatively identified. Principal component analysis illustrated...
Theaflavin-3,3′-digallate (TFDG), a bioactive black tea phenolic, is poorly absorbed in the small intestine, and it has been suggested that gut microbiota metabolism plays crucial role its bioactivities. However, information on metabolic fate impact limited. Here, TFDG was anaerobically fermented vitro by human fecal microbiota, epigallocatechin gallate (EGCG) used for comparison. Despite similar flavan-3-ol skeletons, more slowly degraded yielded distinctively different profile. The...
Green tea catechins are well known for their health benefits. However, these compounds can easily be oxidized, resulting in brown color formation, even the absence of active oxidative enzymes. Browning catechin-rich beverages, such as green tea, during shelf life is undesired. The mechanisms auto-oxidation and products formed still largely unknown. Therefore, we studied auto-oxidative browning epicatechin (EC) epigallocatechin (EGC) model systems. Products EC EGC were analyzed by...
Prenylated flavonoids possess a wide variety of biological activities, including estrogenic, antioxidant, antimicrobial, and anticancer activities. Hence, they have potential applications in food products, medicines, or supplements with health-promoting However, the low abundance prenylated nature is limiting their exploitation. Therefore, we investigated prospect producing yeast Saccharomyces cerevisiae. As proof concept, focused on production potent phytoestrogen 8-prenylnaringenin....
Abstract Fortification of food with iron is considered to be an effective approach counter the global health problem caused by deficiency. However, reactivity catechol moiety phenolics leads discolouration and impairs bioavailability. In this study, we investigated interplay between intrinsic extrinsic factors on iron-catechol complexation. To end, a three-level fractional factorial design was implemented. Absorbance spectra were analysed using statistical methods, including PCA, HCA, ANOVA....
Iron-flavonoid interactions in iron-fortified foods lead to undesirable discolouration. This study aimed investigate iron-mediated complexation, oxidation, and resulting discolouration of flavonoids by spectrophotometric mass spectrometric techniques. At pH 6.5, iron complexation the 3-4 or 4-5 site instantly resulted bathochromic shifting π → π* transition bands, 3'-4' (i.e. catechol moiety) induced a dπ band. Over time, oxidative degradation coupling reactions led formation hydroxybenzoic...
Due to the relatively low bioavailability of tea phenolics in small intestine, their reciprocal interaction with gut microbiota colon may contribute largely beneficial health effects. This implies that be considered as prebiotics. review summarizes current knowledge on metabolic fates from green and black tea, benefits microbial metabolites, potential underlying mechanisms. Additionally, prebiotic effects conventional oligosaccharide prebiotics are compared. Phenolics promptly metabolised...
Analytical techniques, such as liquid chromatography coupled to mass spectrometry (LC-MS) or nuclear magnetic resonance (NMR), are widely used for characterization of complex mixtures (isomeric) proteins, carbohydrates, lipids, and phytochemicals in food. Food can contain isomers that challenging separate, but possess different reactivity bioactivity. Catechins the main phenolic compounds tea; they be present various stereoisomers, which differ their chemical properties. Currently, there is...
Abstract Background Agaricus bisporus is commercially grown on compost, in which the available carbon sources consist mainly of plant-derived polysaccharides that are built out various different constituent monosaccharides. The major monosaccharides these glucose, xylose, and arabinose, while smaller amounts galactose, glucuronic acid, rhamnose mannose also present. Results In this study, genes encoding putative enzymes from metabolism were identified their expression was studied growth...
Avenanthramides are amides, with a phenylalkenoic acid (PA) and an anthranilic (AA) subunit, which secondary metabolites of oat. Oat seeds were germinated, extracted, the avenanthramides analysed by combination UHPLC ion trap high resolution ESI-MS. Typical fragmentation pathways corresponding diagnostic fragments belonging to PA AA subunits identified summarised in decision guideline. Based on these findings 28 unique annotated oat seed(ling) extracts, including new avenanthramide 6f (with...
Auto-oxidation of flavan-3-ols leads to browning and consequently loss product quality during storage ready-to-drink (RTD) green tea. The mechanisms products auto-oxidation galloylated catechins, the major in tea, are still largely unknown. Therefore, we investigated epicatechin gallate (ECg) aqueous model systems. Oxidation tentatively identified based on MS included δ- or γ-type dehydrodicatechins (DhC2s) as main contributors browning. Additionally, various colourless were detected,...
Benzoxazinoids function as defense compounds and have been suggested to possess health-promoting effects. In this work, the mass spectrometric behavior of benzoxazinoids from classes benzoxazin-3-ones (with subclasses lactams, hydroxamic acids, methyl derivatives) benzoxazolinones was studied. Wheat seeds were germinated with simultaneous elicitation by Rhizopus. The seedling extract screened for presence benzoxazinoid (glycosides) using reversed-phase ultra-high-performance liquid...
Synthetic derivatives of 1,4-benzoxazin-3-ones have been shown to possess promising antimicrobial activity, whereas their natural counterparts were found lacking in this respect. In work, quantitative structure-activity relationships (QSAR) and synthetic as antimicrobials established. Data published literature curated into an extensive dataset 111 compounds. Descriptor selection was performed by a genetic algorithm. QSAR models revealed differences requirements for activity against fungi,...
Phenol amides are bioactive compounds naturally present in many plants. This class of is known for antioxidant, anti-inflammatory, and anticancer activities. To better understand the reactivity structure-bioactivity relationships phenol amides, a large set structurally diverse pure needed, however purification from plants inefficient laborious. Existing syntheses require multiple steps, including protection functional groups generally overly complicated only suitable specific combinations...
Abstract Dimethylallyl tryptophan synthases (DMATSs) are aromatic prenyltransferases that catalyze the transfer of a prenyl moiety from donor to an acceptor during biosynthesis microbial secondary metabolites. Due their broad substrate scope, DMATSs anticipated as biotechnological tools for producing bioactive prenylated compounds. Our study explored scope and product profile recombinant RePT, novel DMATS thermophilic fungus Rasamsonia emersonii. Among variety substrates, RePT showed highest...