- Parasitic Infections and Diagnostics
- Parasitic infections in humans and animals
- Helminth infection and control
- Parasite Biology and Host Interactions
- Congenital Anomalies and Fetal Surgery
- Coccidia and coccidiosis research
- Mollusks and Parasites Studies
- Insects and Parasite Interactions
- Pediatric health and respiratory diseases
- Ruminant Nutrition and Digestive Physiology
- Plant and Fungal Interactions Research
- Insect and Pesticide Research
- Parasitic Diseases Research and Treatment
- Forensic Entomology and Diptera Studies
- Ecology and biodiversity studies
- Plant Toxicity and Pharmacological Properties
- European Cultural and National Identity
- Nutrition and Health Studies
- Plant Disease Management Techniques
- Dermatological diseases and infestations
- Central European Literary Studies
- Agriculture and Biological Studies
- Bartonella species infections research
- Mercury impact and mitigation studies
- Insect Pest Control Strategies
Witold Stefański Institute of Parasitology
2008-2020
Polish Academy of Sciences
2008-2020
I.I. Schmalhausen Institute of Zoology
2016
The ability of a reverse line blot (RLB) assay to identify 13 common species equine small strongyles (cyathostomins) and discriminate them from three Strongylus spp. (large strongyles) was demonstrated. relied on the specific hybridization PCR-amplified intergenic spacer DNA fragments nuclear ribosomal membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified simultaneously discriminated each other large (Strongylus edentatus, equinus, vulgaris). This...
A modification of gDNA extraction was developed for the polymerase chain reaction (PCR) technique, intended detection and differentiation Toxocara spp. eggs in soil or sediments. Sand samples from sandpits confirmed as being contaminated with by flotation technique were analysed PCR. The use proteinase K made it possible to obtain genomic DNA sample without needing isolate using inactivate PCR inhibitors present sand. Specific primers allowed discrimination between T. canis cati eggs....
Nematode worms of the genus Trichinella are one most widespread zoonotic pathogens. Natural transmission between hosts can only occur through ingestion infected meat. To date, two species known to be etiological agents disease among domestic animals and wildlife in Poland: T. spiralis britovi. In last decades, since administration an oral vaccination against rabies, red fox population Poland has increased exponentially. The study area covers Nowy Targ region: a mountainous (585-1138 m above...
Abstract To evaluate the gastro-intestinal parasite fauna of wild Polish primitive horses (Equus caballus gmelini Ant., forma silvatica Vet.), 11 yearlings captured in Reserve according to control rules population dynamics were diagnostically dewormed with abamectin+praziquantel. Expelled parasites collected from faeces 24, 36 and 48 hours after treatment. Among a total 4456 specimens (a mean 405.1 per horse) 27 nematode species, one cestode species botfly larvae recovered. Strongylids 100 %...
Abstract The prevalence of Echinococcus multilocularis in 214 red foxes (Vulpes vulpes) the southern part Poland (Małopolskie voivodship) was evaluated post mortem 2005. Infected were found 8 districts within 17 examined, with from 10.0 % to 63.9 (a mean 20.1 whole study area). highest (41.2 and %) revealed two districts, south east province, respectively. high E. region previously recognised as that low parasite endemicity provides evidence for need regular screening current epidemiological...
The objective of this study was to evaluate the risk soil transmitted zoonotic helminth infections for families with young children, inhabitants villages in Mazowieckie Province Central Poland. Epidemiological survey conducted at 33 randomly selected households 2-3 children present. Examination samples from yards surrounding houses presence geohelminth eggs conducted, were inspected, and family members interviewed using a designed questionnaire. Among 55 localities examined, i.e. backyards,...
The level of specific anti-Toxocara IgG was examined in 343 children from central Poland with suspected Toxocara infection. Based on the presence antibodies and clinical signs symptoms, toxocariasis confirmed 249 patients. serological results diagnosed cases were analysed connection epidemiological data (contamination soil around children's homes a dog or cat). A higher prevalence contamination eggs found suburban rural areas (30.9% 24.6%, respectively) whereas it lower urban (10.3%). In...
Summary An extensive analysis of the relationship between age, sex, and different types management strategies relative to gastrointestinal parasite community Polish primitive horses was performed on 124 maintained in nine farms from four regions Poland. The (96 females 28 males) were housed three strategies: stabled (ST), free-ranging (FR) semi-free (SF). These also divided into age groups: <3 years, 3 – 10 years >10 old. parasites collected following deworming all with anthelmintics...
Abstract Toxocariasis is a common human zoonosis, which induces clinically unapparent course of infection. Diagnosis difficult and relies upon serological testing (searching specific IgG antibodies by ELISA), laboratory abnormalities clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection Toxocara canis larvae in host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model toxocariasis. 8 animals inoculated with 1000 T....
Cystic echinococcosis is one of the most important zoonotic diseases affecting humans and livestock worldwide, endemic in Poland. A set six isolates on larval stages Echinococcus granulosus sensu lato tapeworms collected from three humans, two pigs sheep Polish foci CE was examined by DNA sequencing mitochondrial genes (cox1, rrnS). The results demonstrated presence E. canadensis stricto investigated hydatid cysts. former species found all five derived central In a cyst originating Lesser...
Baylisascaris transfuga is a roundworm that has been reported worldwide in most bear species. In mammals and possibly humans, the larvae of B. can migrate tissues aberrant hosts with larva migrans syndrome. The current study was performed to identify faecal samples from free-ranging brown bears Tatra Mountains National Park southern Poland. A commercial kit used extract genomic DNA directly samples. Additionally, Chelex resin-based technique successfully implemented prepare PCR template eggs...