A5064274150- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- CRISPR and Genetic Engineering
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Prenatal Screening and Diagnostics
- Genetic Syndromes and Imprinting
- Reproductive Physiology in Livestock
- Moringa oleifera research and applications
Jiangxi Agricultural University
2021-2023
Chinese Academy of Fishery Sciences
2018-2020
Qingdao National Laboratory for Marine Science and Technology
2020
Shandong Agricultural University
2017
Cytosine base editors (CBEs) and adenine (ABEs) are recently developed CRISPR-mediated genome-editing tools that do not introduce double-strand breaks. In this study, five ABEs, ABE7.10, ABEmax, NG-ABEmax, ABE8e NG-ABE8e, were used to generate A-to-G (T-to-C) conversions in genome loci porcine fetal fibroblasts (PFFs). Variable yet appreciable editing efficiencies variable activity windows observed these targeting regions via editors. The strategy of two sgRNAs one vector exhibited superior...
Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs often unsatisfactory especially when large-size vectors to be delivered. In this study, we aimed optimize conditions delivery a vector in using Lonza 4D-Nucleofector™ vessels and strips.We firstly delivered 13 kb Cas9-EGFP 3.5 pMAX-GFP into via 7 programs recommended by basic protocol. We then tested 6 customized dual-electroporation delivering...
In an attempt to generate g.A746G substitution in the <i>BMPR-IB</i> gene, we unexpectedly obtained homozygous knockout piglets (<i>BMPR-IB</i><sup>-/-</sup>) and heterogeneous with one copy of A746G mutation (<i>BMPR-IB</i><sup>-/746G</sup>) via CRISPR/Cas9 editing. Polymerase chain reaction (PCR) sequencing revealed complex genomic rearrangements target region. All <i>BMPR-IB</i>-disrupted showed inability stand walk normally. Both <i>BMPR-IB</i><sup>-/-</sup>...
Base editing is an efficient and precise gene-editing technique, by which a single base can be changed without introducing double-strand breaks, it currently widely used in studies of various species. In this study, we hA3A-BE3-Y130F to simultaneously introduce premature stop codons (TAG, TGA, TAA) into three tumor suppressor genes, TP53, PTEN, APC, large white porcine fetal fibroblasts (PFFs). Among the isolated 290 single-cell colonies, 232 (80%) had all genes. C−to−T conversion was found...
Summary Cloned animals are prone to abnormal phenotypes such as enlarged tongue, fetal oversize, and progeria. In the present study, whole‐genome bisulfite sequencing mRNA were performed on tongue biceps femoris muscles of cloned piglets with without macroglossia, in an attempt elucidate epigenetic causes macroglossia phenotype. We identified 14 958 18 752 differentially methylated regions muscles, respectively, these correspond 4574 4772 genes compared control group (piglets macroglossia)....