A5001320717- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- Pluripotent Stem Cells Research
- Metal Forming Simulation Techniques
- Sperm and Testicular Function
- Muscle Physiology and Disorders
- Clostridium difficile and Clostridium perfringens research
- RNA and protein synthesis mechanisms
- Animal Behavior and Welfare Studies
- Prenatal Screening and Diagnostics
- Plant Taxonomy and Phylogenetics
Sanya University
2025
Institute of Zoology
2023
Chinese Academy of Sciences
2023
Jiangxi Agricultural University
2016-2022
Abstract Bamaxiang pig is from Guangxi province in China, characterized by its small body size and two‐end black coat colour. It an important indigenous breed for local pork market excellent animal model biomedical research. In this study, we performed genomewide association studies (GWAS) on 43 growth carcass traits 315 purebred pigs based a 1.4 million SNP array. We observed considerable phenotypic variability the pigs. The corresponding heritability varied greatly across ranged 9.0% to...
Gestation length (GL) is a critical indicator of reproductive performance in sows and closely associated with other traits, such as total number born (TNB) alive (NBA). Despite its importance, the genetic mechanisms underlying GL impact on traits remain poorly understood. In this study, we investigated relationship between using 7013 farrowing records conducted an imputed whole-genome sequence-based genome-wide association study (GWAS) for first-parity sows, involving 3005 French Large White...
The accelerated aging of the global population has emerged as a critical public health concern, with increasing recognition influential role played by microbiome in shaping host well-being. Nonetheless, there remains dearth understanding regarding functional alterations occurring within microbiota and their intricate interactions metabolic pathways across various stages aging. This study employed comprehensive metagenomic analysis encompassing saliva stool samples obtained from 45 pigs...
Abstract Wolfberry is well known for its health benefits in Asian countries. This study consisted of two experiments. In Experiment 1, nine boars were provided 40 g dried wolfberry per 100 kg body weight day addition to regular feed 160 days (divided into phases: I, II, III, and IV) under step‐down air temperature conditions. Controls ( n = 9) fed only. Significant p < .05 or .01) slight improvements sperm progressive motility, total abnormality rate, concentration, ejaculate observed the...
p53 is the most frequently mutated gene in human cancers, with over half of all tumors harboring mutation at this locus. R248 and R249 (corresponding to porcine R241 R242), are among hotspot mutations liver, lung, breast, some other cancers. In study, was knocked out or point-edited (R241 R242 were converted 241W 242S) fetal fibroblast (PFF) cells via CRISPR-Cas9 technique. High throughput sequencing miRNA mRNA uncovered a total 225 differentially expressed miRNAs (DEMs) 738 genes (DEGs)...
In an attempt to generate g.A746G substitution in the <i>BMPR-IB</i> gene, we unexpectedly obtained homozygous knockout piglets (<i>BMPR-IB</i><sup>-/-</sup>) and heterogeneous with one copy of A746G mutation (<i>BMPR-IB</i><sup>-/746G</sup>) via CRISPR/Cas9 editing. Polymerase chain reaction (PCR) sequencing revealed complex genomic rearrangements target region. All <i>BMPR-IB</i>-disrupted showed inability stand walk normally. Both <i>BMPR-IB</i><sup>-/-</sup>...
Base editing is an efficient and precise gene-editing technique, by which a single base can be changed without introducing double-strand breaks, it currently widely used in studies of various species. In this study, we hA3A-BE3-Y130F to simultaneously introduce premature stop codons (TAG, TGA, TAA) into three tumor suppressor genes, TP53, PTEN, APC, large white porcine fetal fibroblasts (PFFs). Among the isolated 290 single-cell colonies, 232 (80%) had all genes. C−to−T conversion was found...
Summary Cloned animals are prone to abnormal phenotypes such as enlarged tongue, fetal oversize, and progeria. In the present study, whole‐genome bisulfite sequencing mRNA were performed on tongue biceps femoris muscles of cloned piglets with without macroglossia, in an attempt elucidate epigenetic causes macroglossia phenotype. We identified 14 958 18 752 differentially methylated regions muscles, respectively, these correspond 4574 4772 genes compared control group (piglets macroglossia)....