A5111853748- Plant Pathogens and Fungal Diseases
- Pesticide Residue Analysis and Safety
- Pharmaceutical Quality and Counterfeiting
- Pharmacological Effects and Assays
- Monoclonal and Polyclonal Antibodies Research
- Biosimilars and Bioanalytical Methods
- Analytical chemistry methods development
- Mycorrhizal Fungi and Plant Interactions
- Biosensors and Analytical Detection
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Pharmaceutical and Antibiotic Environmental Impacts
- Malaria Research and Control
- Computational Drug Discovery Methods
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Plant Virus Research Studies
- Pesticide and Herbicide Environmental Studies
- Pharmacological Effects of Natural Compounds
- Glycosylation and Glycoproteins Research
- Folate and B Vitamins Research
- Plant Disease Resistance and Genetics
Guangxi Medicinal Botanical Garden
2019-2025
China Agricultural University
2013-2023
Chinese Academy of Forestry
2012
Malaysian Palm Oil Board
1969-1971
(L.) Urban is a well-known medicinal plant which has multiple pharmacological properties. Notably, the leaves of
Background/Objectives: Illicium verum Hook. f. and difengpi K. I. B.et M. are two important medicinal plants which grow in the mountainous areas of Guangxi, China. Their similar morphological characteristics frequently lead to their misidentification. Chloroplast genome (cp)-based barcode technology has been used effectively identify closely related species; however, at present, there is no systematic comparative study cp sequences between difengpi. Methods: Here, genomes were sequenced...
Objective: Illicium verum is an endemic plant of southern China, which the primary country for its production. Trans-anethole ( t -anethole) key component volatile aromatic essence in I. , and it has therapeutic effects such as anti-cancer anti-diabetes. However, biosynthetic pathway rarely reported. Methods: In present study, we cloned expressed cDNA encoding -anol/isoeugenol synthase (IvAIS1) Escherichia coli . The characteristics IvAIS1 were determined gene expression different tissues...
Artemisinin-based combination therapy (ACT) plays an indispensable role in malaria control and elimination. However, the circulation of counterfeit, substandard drugs has greatly threatened elimination campaigns. Most methods for analysis artemisinin its derivatives require expensive equipment sophisticated instrumentation. A convenient, easy-to-use diagnostic device rapid evaluation quality at point-of-care is still lacking. In this study a lateral flow dipstick immunoassay was developed...
Artemisinin, extracted from Artemisia annua, and its derivatives are important frontline antimalarials. To produce specific antibodies for the detection quantification of artemisinin, artemisinin was transformed to 9-hydroxyartemisinin by microbial fermentation, which used prepare a 9-succinate hapten conjugation with ovalbumin. A monoclonal antibody (mAb), designated as 3H7A10, selected hybridoma cell lines showed high specificity artemisinin. No competitive inhibition observed artesunate,...
Accumulating experimental data have shown that endogenous hormones play important roles in regulating seed dormancy and germination. Zanthoxylum nitidum is a medicinal plant propagates via seeds, which require long period for normal germination, complex changes metabolites occur during the germination process. However, regulatory network of Z. seeds remains unclear. This study investigated dynamic levels seeds. The results revealed an increase gibberellin 3 (GA3), 12-oxophytodienoic acid...
An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 2.0-50.4 ng/mL, respectively. Average recoveries PBZ in wheat (Triticum aestivum) kernel samples were between 84.3 118.9% relative standard deviations 3.9 14.2%. As determined by further confirmed liquid...
Artemether is one of the artemisinin derivatives that are active ingredients in antimalarial drugs. Counterfeit and substandard drugs have become a serious problem, which demands reliable analytical tools implementation strict regulation drug quality. Structural similarity among analogs challenge to develop immunoassays specific derivatives. To produce antibodies artemether, we used microbial fermentation artemether obtain 9-hydroxyartemether, was subsequently prepare 9-O-succinylartemether...
The distribution of metallic ions in plant tissues is associated with their toxicity and important for understanding mechanisms tolerance. A quantitative histochemical method can help advance knowledge cellular subcellular localization heavy metals tissues. An immunohistochemical (IHC) imaging cadmium (Cd2+) was developed the first time wheat Triticum aestivum grown Cd2+-fortified soils. Also, 1-(4-Isothiocyanobenzyl)-ethylenediamine-N,N,N,N-tetraacetic acid (ITCB-EDTA) used to chelate...
Mepiquat chloride (MC) is a plant growth regulator widely used in cotton (Gossypium hirsutum L.) production to suppress excessive vegetative growth, increase root and avoid yield losses. To seeds were treated with MC the number of lateral (LRs) improve drought resistance. An increased indole-3-acetic acid (IAA) pool appeared correlate LR principal source IAA germinating conjugates. Here, role homeostasis signaling was investigated seedlings MC.In present research, significantly endogenous...