A5081365826- Mycobacterium research and diagnosis
- Genomics and Chromatin Dynamics
- Evolution and Genetic Dynamics
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Diphtheria, Corynebacterium, and Tetanus
- RNA modifications and cancer
- Cancer-related gene regulation
- Biotechnology and Related Fields
- Plant Molecular Biology Research
- Gene Regulatory Network Analysis
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- Chromatin Remodeling and Cancer
- Chemistry and Stereochemistry Studies
- RNA Research and Splicing
- COVID-19 epidemiological studies
- Vibrio bacteria research studies
- Tactile and Sensory Interactions
- Plant Disease Resistance and Genetics
- Additive Manufacturing and 3D Printing Technologies
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
Virginia Commonwealth University
2021-2025
Center for Cancer Research
2016-2024
Harvard University
2014-2024
Massachusetts General Hospital
2014-2024
Georgia Institute of Technology
2008-2013
A cDNA encoding a novel human subtilisin-like protease was identified by polymerase chain reaction (PCR) methodology. PCR primers were designed to be specific for the subfamily of eukaryotic proteases with specificity paried basic amino acid residue processing motifs. The gene this protease, designated PACE4, also encoded smaller subtilisin-related polypeptide derived alternate mRNA splicing. deduced PACE4 protein sequence contained number interesting features not present in other family...
The expression dynamics of interacting genes depends, in part, on the structure regulatory networks. Genetic networks include an overrepresentation subgraphs commonly known as network motifs. In this article, we demonstrate that gene copy number is omnipresent parameter can dramatically modify dynamical function We consider positive feedback, bistable and toggle switch motifs show variation number, order a single or few copies, lead to multiple orders magnitude change and, some cases,...
Analysis of The Cancer Genome Atlas and other published data head neck squamous cell carcinoma (HNSCC) reveals somatic alterations the Hippo-YAP pathway in approximately 50% HNSCC. Better strategies to target YAP1 transcriptional complex are sought. Here, we show that FAT1, an upstream inhibitor YAP1, is mutated either by missense or truncating mutation 29% Comprehensive proteomic drug-screening studies across pan-cancer models confirm FAT1-mutant HNSCC exhibits selective higher sensitivity...
Abstract A 3D‐printed origami‐inspired magnetic scaffold has been developed to investigate the influence of physical cues on guided cellular proliferation in a 3D microenvironment. Microscale channels are first constructed and populated with NIH/3T3 fibroblast and/or A549 cancer cell clusters that initially bioprinted within channels. Once these fully populated, permanent magnet is applied fold scaffolds. By varying channel width incorporating an intermediate extracellular matrix hydrogel...
In eukaryotes, the Suv39 family of proteins tri-methylate lysine 9 histone H3 (H3K9me) to form constitutive heterochromatin. However, how are nucleated at heterochromatin is not fully described. fission yeast, current models posit that Argonaute1-associated small RNAs (sRNAs) nucleate sole H3K9 methyltransferase, Clr4/SUV39H, centromeres. Here, we show in absence all sRNAs and H3K9me, Mtl1 Red1 core (MTREC)/PAXT complex nucleates Clr4/SUV39H a heterochromatic long noncoding RNA (lncRNA)...
Cell fate determination is usually described as the result of stochastic dynamics gene regulatory networks (GRNs) reaching one multiple steady-states each which corresponds to a specific decision. However, cell determined in finite time suggesting importance transient cellular decision making. Here we consider making resulting from first passage processes proteins and examine effect within initial lysis-lysogeny switch phage λ. Importantly, an infected depends, part, on number coinfecting...
Although the length and constituting sequences for pericentromeric repeats are highly variable across eukaryotes, presence of multiple is one conserved features eukaryotic chromosomes. Pericentromeric heterochromatin often misregulated in human diseases, with expansion solid cancers. In this article, we have developed a mathematical model RNAi-dependent methylation H3K9 region fission yeast. Our model, which takes copy number as an explicit parameter, predicts that pericentromere silenced...
Shigellosis, a diarrheal disease, is endemic worldwide and responsible for approximately 15,000 laboratory-confirmed cases in the United States every year. However, patients with shigellosis often do not seek medical care. To estimate burden of shigellosis, we extended time-series susceptible-infected-recovered models to infer epidemiologic parameters from underreported case data. We applied susceptible-infected-recovered–based inference schemes analyze largest surveillance data set Shigella...
Abstract 3D printed platforms have diverse possible applications in cell‐based assays, creating biomimetic tissue or organ microenvironments, delineating cell‐to‐cell, cell‐to‐matrix, cell‐to‐local microenvironment interactions, and investigating drug discovery. Existing engineering techniques limit physiologically relevant cell proliferation spatiotemporal organization of cells. Herein, a facile fabrication strategy is proposed for magnetically controllable, shape‐morphing,...
Circadian rhythms are important biological contributors to health. Rest activity (RAR) emerging as biomarkers of circadian behavior that associated with chronic disease when abnormal. RAR have not yet been characterized in kidney diseases (CKD). Leveraging the National Health and Nutrition Examination Survey (2011-2014), patients CKD (
The fission (Schizosaccharomyces pombe) and budding (Saccharomyces cerevisiae) yeasts have served as excellent models for many seminal discoveries in eukaryotic biology. In these organisms, genes are deleted or tagged easily by transforming cells with PCR-generated DNA inserts, flanked short (50-100bp) regions of gene homology. These PCR reactions use especially designed long primers, which, addition to the priming sites, carry homology targeting. Primer design follows a fixed method but is...
Abstract Identifying genes that are aberrantly expressed is an important first step in the diagnosis and treatment of many diseases. Conventionally, differential expression (DE) analysis used to screen gene profiles identify functionally associated genes. DE often relies on variance fold change from individual genes, which does not consider all other within profile. When overall skewed, capture outliers expression. To address this, we have developed a non-parametric method based probability...