A5050276158- CRISPR and Genetic Engineering
- Innovation and Socioeconomic Development
- Mosquito-borne diseases and control
- Cancer Cells and Metastasis
- RNA and protein synthesis mechanisms
- Biomedical and Engineering Education
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Virus-based gene therapy research
- Neonatal Respiratory Health Research
- Advanced biosensing and bioanalysis techniques
- Medical Imaging and Pathology Studies
Southern Medical University
2022-2023
Abstract Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system. Features such as recognition of T-rich protospacer-adjacent motif (PAM) and generation sticky breaks, well amenability for multiplex editing in single crRNA lower off-target nuclease activity, broaden targeting scope available tools enable more accurate genome editing. However, widespread use gene editing, especially clinical applications,...
Human adenoviruses type 3 (HAdV-3) and 55 (HAdV-55) are frequently encountered, highly contagious respiratory pathogens with high morbidity rate. In contrast to HAdV-3, one of the most predominant types in children, HAdV-55 is a reemergent pathogen associated more severe community-acquired pneumonia (CAP) adults, especially military camps. However, infectivity pathogenicity differences between these viruses remain unknown as vivo models not available. Here, we report novel system utilizing...
Abstract Background The CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research hold great potential future clinical applications. However, the short half-life of guide RNAs (gRNAs), particularly free gRNAs without Cas nuclease binding, limits their editing efficiency durability. Results Here, we engineer circular (cgRNAs) to increase stability, thus availability Cas12a Cas13d processing loading, boost editing. cgRNAs increases Cas12a-based transcription activators...
Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. In study, we made parallel comparison between recently reported miniature Cas12f1 (Un1Cas12f1 AsCas12f1) Cas12a Cas9 nucleases in mammalian cells. We found that as CRISPRa activator, Un1Cas12f1 could induce gene expression with comparable level to Cas9, while cleavage editor, exhibited similar properties Cas12a,...
Abstract Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. Herein we made parallel comparison between recently reported miniature Cas12f1 Cas12a Cas9 nucleases in mammalian cells. The results revealed that as CRISPRa activator, Un1Cas12f1 could induce gene expression with comparable level to these Cas9, while cleaving editor, exhibited similar properties Cas12a,...
Abstract Background Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. Herein we made parallel comparison between recently reported miniature Cas12f1 Cas12a Cas9 nucleases in mammalian cells. Results We found that as CRISPRa activator, Un1 could induce gene expression with comparable level to these Cas9, while cleaving editor, exhibited similar properties Cas12a,...