A5072780850- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Innovation and Socioeconomic Development
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Genomics and Chromatin Dynamics
- Kruppel-like factors research
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Immunodeficiency and Autoimmune Disorders
- Protein Degradation and Inhibitors
- Viral Infections and Immunology Research
- Genetics, Aging, and Longevity in Model Organisms
- Dermatologic Treatments and Research
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Wound Healing and Treatments
- Genomics and Phylogenetic Studies
- Psoriasis: Treatment and Pathogenesis
- Cancer-related gene regulation
- Plant Virus Research Studies
- Virus-based gene therapy research
- IL-33, ST2, and ILC Pathways
- Nanoplatforms for cancer theranostics
Southern Medical University
2017-2025
First Affiliated Hospital of Guangzhou Medical University
2025
Guangzhou Medical University
2025
State Key Laboratory of Respiratory Disease
2025
Southern Medical University Shenzhen Hospital
2020-2023
Abstract Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system. Features such as recognition of T-rich protospacer-adjacent motif (PAM) and generation sticky breaks, well amenability for multiplex editing in single crRNA lower off-target nuclease activity, broaden targeting scope available tools enable more accurate genome editing. However, widespread use gene editing, especially clinical applications,...
Liquid-liquid phase separation (LLPS) plays a critical role in regulating gene transcription via the formation of transcriptional condensates. However, LLPS has not been reported to be engineered as tool activate endogenous expression mammalian cells or vivo. Here, we developed droplet-forming CRISPR (clustered regularly interspaced short palindromic repeats) activation system (DropCRISPRa) with high efficiency combining CRISPR-SunTag FETIDR-AD fusion proteins, which contain an N-terminal...
Abstract Background The CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research hold great potential future clinical applications. However, the short half-life of guide RNAs (gRNAs), particularly free gRNAs without Cas nuclease binding, limits their editing efficiency durability. Results Here, we engineer circular (cgRNAs) to increase stability, thus availability Cas12a Cas13d processing loading, boost editing. cgRNAs increases Cas12a-based transcription activators...
CRISPR system has been widely used due to its precision and versatility in gene editing. Un1Cas12f1 from uncultured archaeon (hereafter referred as Cas12f), known for compact size (529 aa), exhibits obvious delivery advantage editing vitro vivo. However, activity remains suboptimal. In this study, we engineer circular guide RNA (cgRNA) Cas12f significantly improve the efficiency of activation about 1.9–19.2-fold. When combined with a phase separation system, is further increased...
The CRISPR/Cas system is widely used for genome editing. However, robust and targeted insertion of a DNA segment remains challenge. Here, we present fusion nuclease (Cas9-N57) to enhance site-specific integration via fused binding domain Sleeping Beauty transposase tether the Cas9/sgRNA complex. was unidirectional specific, fragments up 12 kb in length were successfully integrated. As test system, Cas9-N57 mediated CD19-specific chimeric antigen receptor (CD19-CAR) cassette into AAVS1 locus...
Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. In study, we made parallel comparison between recently reported miniature Cas12f1 (Un1Cas12f1 AsCas12f1) Cas12a Cas9 nucleases in mammalian cells. We found that as CRISPRa activator, Un1Cas12f1 could induce gene expression with comparable level to Cas9, while cleavage editor, exhibited similar properties Cas12a,...
Abstract Keratinocyte hyperproliferation is a key pathogenic factor in psoriasis. However, the mechanisms that regulate keratinocyte this condition remain unclear. Here, we found SLC35E1 was highly expressed keratinocytes of patients with psoriasis and Slc35e1 − / mice displayed less severe imiquimod (IMQ)-induced psoriasis-like phenotype than their wild-type siblings. In addition, deficiency inhibited proliferation both cultured cells. On molecular level, to zinc ion concentrations...
Ultrasmall gold glyconanoparticles with enhanced tumor-targeting efficiency and efficient clearance through both renal hepatobiliary pathways.
Abstract Genome-wide identification of DNA double-strand breaks (DSBs) induced by CRISPR-associated protein (Cas) systems is vital for profiling the off-target events Cas nucleases. However, current methods discovery are tedious and costly, restricting their widespread applications. Here we present an easy alternative method CRISPR detection tracing integrated oligonucleotide Tag using next-generation- sequencing (CRISPR-Tag-seq, or Tag-seq). Tag-seq enables rapid convenient nuclease-induced...
Abstract Background Keloids represent one extreme of aberrant dermal wound healing and are characterized by fibroblast hyperproliferation excessive deposition extracellular matrix. Genetics is a major factor for predisposition to keloids genome-wide association study has identified single-nucleotide polymorphism (SNP) rs873549 at 1q41 as susceptibility locus. The SNP rs873549, the SNPs in strong linkage disequilibrium (LD) with may be involved keloid development. However, functional...
Human embryonic stem cells (hESCs) have a wide range of applications in early human development mimics, disease modeling, and cell therapy. To fulfill these applications, we established hESCs for inducible multiplex orthogonal gene knockout activation, which named iKA-CRISPR hESCs. In cells, when complexed with short guide RNA containing 14-bp target sequence (14-bp gRNA) or long 20-bp gRNA, the doxycycline-induced Cas9-p300 protein could activate transcription cleave genomic DNA,...
Abstract Genome-wide mapping of chromatin interactions at high resolution remains experimentally and computationally challenging. Here we used a low-input “easy Hi-C” (eHi-C) protocol to map the 3D genome architecture in neurogenesis brain tissues, also developed an improved Hi-C bias-correction pipeline ( HiCorr ) enabling better identification enhancer loops or aggregates sub-TAD level. We compared ultra-deep maps from 10 human tissue- cell types, with focus on stem cells neural...
Abstract Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. Herein we made parallel comparison between recently reported miniature Cas12f1 Cas12a Cas9 nucleases in mammalian cells. The results revealed that as CRISPRa activator, Un1Cas12f1 could induce gene expression with comparable level to these Cas9, while cleaving editor, exhibited similar properties Cas12a,...
Abstract Background Profiling and comparing the performance of current widely used DNA targeting CRISPR systems provide basic information for gene-editing toolkit can be a useful resource this field. Herein we made parallel comparison between recently reported miniature Cas12f1 Cas12a Cas9 nucleases in mammalian cells. Results We found that as CRISPRa activator, Un1 could induce gene expression with comparable level to these Cas9, while cleaving editor, exhibited similar properties Cas12a,...