A5059862828- Clinical Laboratory Practices and Quality Control
- Acute Myeloid Leukemia Research
- AI in cancer detection
- Digital Imaging for Blood Diseases
- Hematopoietic Stem Cell Transplantation
- Platelet Disorders and Treatments
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Colorectal Cancer Screening and Detection
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Oral Health Pathology and Treatment
- Polymer Nanocomposite Synthesis and Irradiation
- Anatomy and Medical Technology
- Blood groups and transfusion
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Biomedical Text Mining and Ontologies
- Head and Neck Cancer Studies
- Eosinophilic Disorders and Syndromes
- Radiomics and Machine Learning in Medical Imaging
Manchester University NHS Foundation Trust
2015-2019
Manchester Royal Infirmary
1996-2016
Foundation University Medical College
2016
King Saud University
2016
Universitat Politècnica de Catalunya
2016
Achievers University
2016
University of Manchester
2014
St George Hospital
2014
South Eastern Sydney Local Health District
2014
Royal Manchester Children's Hospital
2005
Summary We report the results of a pilot study assessing use digital ‘virtual slides’ in haematological quality assessment. Conducted together with UK National External Quality Assessment Scheme for General Haematology, involved 166 separate participants, using format typical assessment exercise. The revealed substantial concordance observations made slides those reported previous glass slide surveys that used identical cases. Participant feedback strongly supported electronic teaching and...
The laboratory interpretation of blood film morphology is frequently a rapid, accurate, and cost-effective final-stage count analysis. However, the findings often rests with single individual, errors can carry significant impact. Cell identification classification skills are well supported by existing resources, but contribution importance other less understood.The UK external quality assurance group in haematology (UK NEQAS(H)) runs Continued Professional Development scheme where large...
Mpl ligand is a recently cloned haemopoietic growth factor that stimulates megakaryopoiesis in vitro and vivo . We describe the effect of truncated form ligand, recombinant human megakaryocyte development (rHuMGDF), on bone marrow from normal subjects patients with myelodysplastic syndrome (MDS) acute myeloid leukaemia (AML). used both semi‐solid suspension culture techniques to assess pegylated (PEG) rHuMGDF colony (CFU‐Mk) production CD61 + cells 7 d cultures. PEG increased CFU‐Mk cell...
Background: Morphological examination of blood films remains the reference standard for malaria diagnosis. Supporting skills required to make an accurate morphological diagnosis is therefore essential. However, providing support across different countries and environments a substantial challenge.
Summary UK NEQAS (H) developed and instigated a pilot scheme for digital morphology, which was accessed by participants over the internet in order to assess viability of using high quality images as an educational tool continuing professional development. The trialled 2‐year period with eight releases totalling 16 morphology cases. Digital allowed participating individuals examine comment on exactly same cells compare their findings those other participants, consensus data from traditional...
The effects of recombinant macrophage inflammatory protein 1α (rhMIP‐1α) on the proliferation leukaemic blast cells from patients with acute myeloid leukaemia was assessed. Using previously described [ 3 H]thymidine incorporation index assay, response autonomous and growth factor responsive AML to chemokine rhMIP‐1 measured. In case proliferators, rhMIP‐1α had no inhibitory effect in 4/6 cases H]‐thymidine stimulated by rhMIP‐1α. presence stem cell (SCF), a majority (8/9) samples which...
Patients in long‐term remission of acute myeloid leukaemia (AML) M2 with t(8;21) after chemotherapy, or without bone marrow transplantation, are known to retain residual cells which express AML1/MTG8 transcripts marrow, detectable by RT‐PCR. In order determine whether these clonogenic, we have grown samples standard semi‐solid culture and picked individual CFU‐GM BFU‐E colonies were then analysed for the expression using a rapid specific RT‐PCR technique. Nine patients tested remission, six...
Pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) can stimulate megakaryopoiesis in vitro some myelodysplastic syndrome (MDS) acute myeloid leukaemia (AML) patients. We assessed PEG-rHuMGDF combined with granulocyte colony-stimulating (G-CSF), granulocyte-macrophage CSF (GM-CSF), interleukin 3 (IL-3), IL6, stem cell (SCF) or erythropoietin 40 MDS, 33 AML 16 normal bone marrow samples. CD61-positive cells suspension cultures increased alone 20/25 RA + RAS,...
Recombinant human megakaryocyte growth and development factor (rHuMGDF), a truncated form of the Mpl ligand, stimulates megakaryopoiesis both in vitro vivo . We describe effect pegylated recombinant MGDF (PEGrHuMGDF) alone combination with other haemopoietic factors (G‐CSF, GM‐CSF, IL3, IL6, erythropoietin, SCF) on bone marrow from 11 normal subjects 19 patients aplastic anaemia (AA). used semi‐solid cultures to assess colony (CFU‐Mk) 7 d suspension production platelet glycoprotein IIIa...
Background The recognition and interpretation of abnormal blood cell morphology is often the first step in diagnosing underlying serious systemic illness or leukemia. Supporting staff who interpret film therefore essential for a safe laboratory service. This paper describes an open-access, web-based decision support tool, developed by authors to morphological diagnosis, arising from earlier studies identifying mechanisms error reporting. effectiveness this intervention was assessed using...
Long‐term bone marrow culture (LTBMC) has been used successfully in autologous transplantation chronic myeloid leukaemia (CML). However, variation between patients the recovery of Ph − cells limits application this procedure to a minority. Treatment that effectively reduces vivo tumour burden prior initiation LTBMC may improve selection culture. To test hypothesis we evaluated frequency and degree cytogenetic conversion haemopoiesis from four independent groups CML patients: Untreated ( n =...
We have adapted the alkaline phosphatase-anti phosphatase (APAAP) technique to demonstrate cell antigen distributions in intact agar culture. The method facilitates batch processing and is no less convenient perform than standard APAAP procedures. Myeloid lymphoid antigens generally demonstrated strong staining intensity. However, at day 0 consistently produced expression for two monoclonals (CD11c CD34) contrast positivity parallel cytospins. CD11c showed rapidly increasing over subsequent...
We have studied paired peripheral blood progenitor cells (PBPC) and bone marrow (BM) samples from 12 acute myeloid leukaemia (AML) patients following intensive chemotherapy, assessed direct granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming (BFU-E), megakaryocyte CFU (CFU-Mk) numbers the production of CD61+ (platelet glycoprotein IIIa) in suspension culture response to various haemopoietic growth factor combinations. found that CFU-GM BFU-E per 105 mononuclear were...
Abstract Background Screening for malaria in the returning traveler has often required repeat testing; however, audit data suggest that patients have not been reattending. We sought to ascertain if this was safe by examining diagnostic efficacy of a single screen consisting rapid test (RDT) and thin film. Methods conducted retrospective cohort study with suspected who attended past 5 years from two large teaching hospitals. assessed accuracy screen, reporting measures sensitivity...
Introduction: Abnormal lymphoid cells and blast are mononuclear (MNC) circulating in peripheral blood (PB) malignant diseases such as neoplasms acute leukemias.The goal of this work is to build a simple fast classifier recognize automatically among three groups images obtained from PB: 1) MNC 2) granulocytes (G) 3) smudge (SC).This necessary step approach more accurately the segmentation automatic classification MNC.Methods: We created database containing total 57008 digital cell PB 404...