A5013878081- Retinal Diseases and Treatments
- Mitochondrial Function and Pathology
- Sphingolipid Metabolism and Signaling
- Optical Imaging and Spectroscopy Techniques
- Sirtuins and Resveratrol in Medicine
- ATP Synthase and ATPases Research
- Biomedical Research and Pathophysiology
- Biochemical effects in animals
- Food Quality and Safety Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Radiation Effects and Dosimetry
- Lipid Membrane Structure and Behavior
- Retinopathy of Prematurity Studies
- Advanced MRI Techniques and Applications
- melanin and skin pigmentation
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Adipose Tissue and Metabolism
- Electrochemical sensors and biosensors
- Neurological Disorders and Treatments
- Enzyme-mediated dye degradation
- Sleep and Wakefulness Research
- Analytical Chemistry and Sensors
- Dialysis and Renal Disease Management
- Plant Genetic and Mutation Studies
Michigan State University
2016-2024
Michigan United
2016-2020
Brooklyn College
2011-2012
City University of New York
2011
Certain fungi thrive in highly radioactive environments including the defunct Chernobyl nuclear reactor. Cryptococcus neoformans (C. neoformans), which uses L-3,4-dihydroxyphenylalanine (L-DOPA) to produce melanin, was used here investigate how gamma radiation under aqueous aerobic conditions affects properties of with aim gaining insight into its radioprotective role. Exposure melanized fungal cell suspensions doses γ-radiation capable killing 50 80% cells did not lead a detectable loss...
Abstract Mitochondrial reactive oxygen species (ROS) play important roles in cellular signaling; however, certain pathological conditions such as ischemia/reperfusion (I/R) injury disrupt ROS homeostasis and contribute to cell death. A major impediment developing therapeutic measures against oxidative stress-induced damage is the lack of a quantitative framework identify specific sources regulatory mechanisms mitochondrial production. We developed thermodynamically consistent,...
Mitochondrial damage in the cells comprising inner (retinal endothelial cells) and outer pigment epithelium (RPE)) blood–retinal barriers (BRB) is known to precede initial BRB breakdown further histopathological abnormalities diabetic retinopathy (DR). We previously demonstrated that activation of acid sphingomyelinase (ASM) an important early event pathogenesis DR, recent studies have there intricate connection between ceramide mitochondrial function. This study aimed determine role...
Catalase activity of the dual-function heme enzyme catalase-peroxidase (KatG) depends on several structural elements, including a unique adduct formed from covalently linked side chains three conserved amino acids (Met-255, Tyr-229, and Trp-107, Mycobacterium tuberculosis KatG numbering) (MYW). Mutagenesis, electron paramagnetic resonance, optical stopped-flow experiments, along with calculations using density functional theory (DFT) methods revealed basis requirement for radical MYW-adduct,...
Oxygen consumption is a key metric of metabolism in aerobic organisms. Current respirometric methods led to seminal discoveries despite limitations such as high sample demand, exchange with atmospheric O2, and cumulative titration protocols leading limited choice useable tissue, complex data interpretation, restricted experimental design. We developed sensitive customizable method measuring O2 rates by variety biological samples microliter volumes without interference from the environment....
Mitochondrial isolation has been practiced for decades, following procedures established by pioneers in the fields of molecular biology and biochemistry to study metabolic impairments disease. Consistent mitochondrial quality is necessary properly investigate physiology bioenergetics; however, many different published methods are available researchers. Although experimental strategies require methods, basic principles similar. This protocol details a method capable extracting well-coupled...
Abstract Mitochondrial reactive oxygen species (ROS) play important roles in cellular signaling; however, certain pathological conditions such as ischemia/reperfusion (I/R) injury disrupt ROS homeostasis and contribute to cell death. A major impediment developing therapeutic measures against oxidative stress induced damage is the lack of a quantitative framework identify specific sources regulatory mechanisms mitochondrial production. We developed thermodynamically consistent,...
Mitochondrial damage precedes histopathological abnormalities in diabetic retinopathy (DR). Retinal endothelial cells (REC) have the highest level of Acid Sphingomyelinase (ASM) expression retina and increase ASM activity ceramide production were shown to promote diabetes-induced pro-inflammatory pro-apoptotic changes REC. The role mitochondrial REC is not known. mitochondria from control, STZ-induced ASM-/- rodent models, as well Bovine (BREC) treated with diabetogenic conditions isolated...
Summary In diabetes, the retina, a tissue with unique metabolic needs, demonstrates dysregulation of intricate balance between nutrient availability and utilization. This results in cholesterol accumulation, pro-inflammatory pro-apoptotic changes, consequently neurovascular damage. Sirtuin 1 (SIRT1), sensing deacetylase, is downregulated diabetic retina. this study, effect SIRT1 stimulation by fasting or pharmacological activation using SRT1720, was evaluated on retinal metabolism,...