A5008182988- Viral Infections and Immunology Research
- Biochemical effects in animals
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Cardiomyopathy and Myosin Studies
- Mycobacterium research and diagnosis
- Cardiovascular Effects of Exercise
- Metabolism, Diabetes, and Cancer
- Aldose Reductase and Taurine
- Free Radicals and Antioxidants
- Retinal Diseases and Treatments
- Cytomegalovirus and herpesvirus research
- Antibiotic Resistance in Bacteria
- Analytical Chemistry and Sensors
- Ginseng Biological Effects and Applications
- Eosinophilic Disorders and Syndromes
- Bacterial Genetics and Biotechnology
- Biomedical Text Mining and Ontologies
- Biomedical Research and Pathophysiology
- Transplantation: Methods and Outcomes
- RNA modifications and cancer
- Liver physiology and pathology
- Stress Responses and Cortisol
- Retinopathy of Prematurity Studies
- Liver Disease Diagnosis and Treatment
Heidelberg University
1992-2024
Heinrich Heine University Düsseldorf
2018-2024
University Hospital Heidelberg
1997-2024
Michigan State University
2018-2019
Michigan United
2018
Medizinische Hochschule Hannover
1992-1994
Max Planck Institute of Biochemistry
1988-1992
University of Bayreuth
1988
Max Planck Society
1988
We have developed an in situ hybridization assay capable of detecting enteroviral RNA myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because the high degree nucleic acid sequence homology among numerous serotypes, including group A and B coxsackieviruses echoviruses, detection these various agents commonly implicated human viral heart disease is possible single assay. demonstrate considerable potential this method for unequivocal diagnosis well...
We report on the identification of mutations associated with streptomycin resistance in Mycobacterium tuberculosis. Two isolates (3656 and 3976) showed a wild-type ribosomal protein, S12, but exhibited single point mutation at 16S rRNA position 491 (C-->T) or 512 (C-->T), respectively. Sequence analysis third isolate (2438) revealed base change 904 (A-->G). This is equivalent to invariant 913 Escherichia coli gene, an A-->G transition which has been shown previously impair...
Abstract Recently, we have shown that after partial hepatectomy (PHx), an increased hepatic blood flow initiates liver growth in mice by vasodilation and mechanically-triggered release of angiocrine signals. Here, use mass spectrometry to identify a mechanically-induced signal human endothelial cells, is, myeloid-derived factor (MYDGF). We show it induces proliferation promotes survival primary hepatocytes derived from different donors two-dimensional cell culture, via activation...
Prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as non-alcoholic fatty disease, increases worldwide and associates with type 2 diabetes other cardiometabolic diseases. Here we demonstrate that Sema3a is elevated in sinusoidal endothelial cells animal models for obesity, MASLD. In primary human cells, saturated acids induce expression SEMA3A, loss a single allele sufficient to reduce hepatic fat content diet-induced obese mice. We show...
The antiviral effects of human interferon-β (IFN-β) and recombinant interferon-γ (rIFN-γ) were studied in persistently coxsackievirus B3-infected carrier cultures myocardial fibroblasts over a period 21 days. Synergism was observed with concentrations as low 30 IU IFN-β plus 10 rIFN-γ/mL, reducing mean viral titers from 6.0 × 107 to 1.3 104 pfu/mL number infected cells 14.4% 0.1% determined by quantitative situ hybridization. Higher IFNs (both ⩾ IU/mL) associated transient antagonism...
Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differentiated biochemically described Mycobacterium species, although it most closely resembled malmoense by thin-layer chromatography high-performance liquid mycolic acids. striking...
Abstract Aim Although of potential biomedical relevance, dipeptide metabolism has hardly been studied. We found the dipeptidase carnosinase‐2 (CN2) to be abundant in human proximal tubules, which regulate water and solute homeostasis. therefore hypothesized, that CN2 a key metabolic role, impacting tubular transport function. Methods A knockout gene ( CNDP2 ‐KO) was generated tubule cells characterized by metabolomics, RNA‐seq analysis, paracellular permeability analysis ion transport....
Type 2 diabetes is characterized by insulin hypersecretion followed reduced glucose-stimulated secretion (GSIS). Here we show that acute stimulation of pancreatic islets with the secretagogue dextrorphan (DXO) or glibenclamide enhances GSIS, whereas chronic treatment high concentrations these drugs reduce GSIS but protect from cell death. Bulk RNA sequencing shows increased expression genes for serine-linked mitochondrial one-carbon metabolism (OCM) after chronic, not acute, stimulation. In...
Carnosine and anserine supplementation markedLy reduce diabetic nephropathy in rodents. The mode of nephroprotective action both dipeptides diabetes, via local protection or improved systemic glucose homeostasis, is uncertain. Global carnosinase-1 knockout mice (Cndp1-KO) wild-type littermates (WT) on a normal diet (ND) high fat (HFD) (n = 10/group), with streptozocin (STZ)-induced type-1 diabetes 21-23/group), were studied for 32 weeks. Independent diet, Cndp1-KO had 2- to 10-fold higher...
Oxygen consumption is a key metric of metabolism in aerobic organisms. Current respirometric methods led to seminal discoveries despite limitations such as high sample demand, exchange with atmospheric O2, and cumulative titration protocols leading limited choice useable tissue, complex data interpretation, restricted experimental design. We developed sensitive customizable method measuring O2 rates by variety biological samples microliter volumes without interference from the environment....
Journal Article Cultured human heart cells: a model system for the study of antiviral activity interferons Get access R. Kandolf, Kandolf Search other works by this author on: Oxford Academic PubMed Google Scholar P. Kirschner, Kirschner D. Ameis, Ameis A. Canu, Canu H. Hofschneider European Heart Journal, Volume 8, Issue suppl_J, November 1987, Pages 453–456, https://doi.org/10.1093/eurheartj/8.suppl_J.453 Published: 01 1987
Background Exogenous carnosine and anserine mitigate diabetic nephropathy in rodents, whether they exert direct renal or indirect systemic effects is unknown. We studied carnosinase-1 KO (Cndp1-KO) mice with selectively 2-9-fold increased carnosine/anserine concentrations kidney under conditions.