A5068990029- Prostate Cancer Treatment and Research
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Renal cell carcinoma treatment
- Prostate Cancer Diagnosis and Treatment
- Renal and related cancers
- Ubiquitin and proteasome pathways
- Cervical Cancer and HPV Research
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- 14-3-3 protein interactions
- Molecular Biology Techniques and Applications
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- PARP inhibition in cancer therapy
- Multiple Myeloma Research and Treatments
- Gastrointestinal Tumor Research and Treatment
- Bladder and Urothelial Cancer Treatments
- Chronic Myeloid Leukemia Treatments
- Ferroptosis and cancer prognosis
- Immunotherapy and Immune Responses
- Connective Tissue Growth Factor Research
- Genetic factors in colorectal cancer
- FOXO transcription factor regulation
Heidelberg University
2015-2024
University Hospital Heidelberg
2015-2024
National Center for Tumor Diseases
2014-2024
UPMC Hillman Cancer Center
2004-2023
University of Pittsburgh
2005-2023
Roth and Rau (Germany)
2018
Chirurgische Universitätsklinik Heidelberg
2012-2017
Taipei Institute of Pathology
2014
Weatherford College
2012
Oregon Health & Science University
2010
The prostate-specific membrane antigen (PSMA) targeted positron-emitting-tomography (PET) tracer 68Ga-PSMA-11 shows great promise in the detection of prostate cancer. However, 68Ga has several shortcomings as a radiolabel including short half-life and non-ideal energies, this motivated consideration 18F-labelled analogs. 18F-PSMA-1007 was selected among 18F-PSMA-ligand candidate compounds because it demonstrated high labelling yields, outstanding tumor uptake fast, non-urinary background...
Loss of genomic integrity is a defining feature many human malignancies, including papillomavirus (HPV)-associated preinvasive and invasive genital squamous lesions. Here we show that aberrant mitotic spindle pole formation caused by abnormal centrosome numbers represents an important mechanism in accounting for numeric chromosomal alterations HPV-associated carcinogenesis. Similar to what found histopathological specimens, HPV-16 E6 E7 oncoproteins cooperate induce numbers, formation,...
Introduction Artificial intelligence (AI) is increasingly used in healthcare. AI-based chatbots can act as automated conversational agents, capable of promoting health and providing education at any time. The objective this study was to develop evaluate a user-friendly medical chatbot (prostate cancer communication assistant (PROSCA)) for provisioning patient information about early detection prostate (PC). Methods developed provide on diseases, diagnostic tests PC detection, stages,...
Several drugs approved for a variety of indications have been shown to exhibit antiangiogenic effects. Our study focuses on the PPARγ ligand rosiglitazone, compound widely used in treatment type 2 diabetes. We demonstrate, first time our knowledge, that is highly expressed tumor endothelium and activated by rosiglitazone cultured endothelial cells. Furthermore, we show suppresses primary growth metastasis both direct indirect Rosiglitazone inhibits bovine capillary cell but not proliferation...
Several drugs approved for a variety of indications have been shown to exhibit antiangiogenic effects. Our study focuses on the PPARγ ligand rosiglitazone, compound widely used in treatment type 2 diabetes. We demonstrate, first time our knowledge, that is highly expressed tumor endothelium and activated by rosiglitazone cultured endothelial cells. Furthermore, we show suppresses primary growth metastasis both direct indirect Rosiglitazone inhibits bovine capillary cell but not proliferation...
We report the characterization of Cep170, a forkhead-associated (FHA) domain protein previously unknown function. Cep170 was identified in yeast two-hybrid screen for interactors Polo-like kinase 1 (Plk1). In human cells, is constantly expressed throughout cell cycle but phosphorylated during mitosis. It interacts with Plk1 vivo and can be by vitro, suggesting that it physiological substrate this kinase. Both overexpression small interfering RNA (siRNA)-mediated depletion studies suggest...
Background: Stratifying PCa patients into risk groups at time of initial diagnosis enabling a risk-adapted disease management is still major clinical challenge. Existing studies evaluating the prognostic potential PSMA for were performed on radical prostatectomy specimens (RPE), i.e. decision making was already completed sample analysis. Aim our study to assess value (prostate-specific membrane antigen) expression prostate cancer (PCa) biopsies diagnosis. Methods: assessed by...
68Ga-prostate-specific membrane antigen (PSMA)-11 PET/CT represents an advanced method for the staging of primary prostate cancer (PCa) and diagnosis recurrent or metastatic PCa. However, because narrow availability 68Ga development alternative tracers is high interest. The objective this study was to examine value new PET tracer 18F-PSMA-1007 local disease by comparing it with multiparametric MRI (mpMRI) radical prostatectomy (RP) histopathology. Methods: In 2016, performed in 10 men...
Purpose: The Mediator complex is a multiprotein assembly, which serves as hub for diverse signaling pathways to regulate gene expression. Because expression frequently altered in cancer, systematic understanding of the malignancies could foster development novel targeted therapeutic approaches.Experimental Design: We performed deconvolution subunit profiles across 23 cancer entities (n = 8,568) using data from Cancer Genome Atlas (TCGA). Prostate cancer-specific findings were validated two...
Human papillomaviruses (HPVs) are small circular DNA viruses that cause warts. Infection with high-risk anogenital HPVs, such as HPV type 16 (HPV16), is associated human cancers, specifically cervical cancer. The life cycle of HPVs intimately tied to the differentiation status host epithelium and has two distinct stages: nonproductive stage productive stage. In stage, which arises in poorly differentiated basal epithelial compartment a wart, virus maintains itself low-copy-number nuclear...
The importance of mitochondria as oxygen sensors well producers ATP and reactive species (ROS) has recently become a focal point cancer research. However, in the case melanoma, little information is available to what extent cellular bioenergetics processes contribute progression disease related it, whether oxidative phosphorylation (OXPHOS) prominent role advanced melanoma. In this study we demonstrate that compared melanocytes, metastatic melanoma cells have elevated levels OXPHOS....
The human papillomavirus (HPV) 16 E7 oncoprotein has been reported previously to stimulate DNA damage and activate host cell checkpoints. How HPV-16 maintains proliferation despite activated checkpoints is incompletely understood. Here, we provide evidence that cells expressing the can enter mitosis in presence of damage. We show this activity involves attenuation checkpoint control by accelerating proteolytic turnover claspin. Claspin mediates activation CHK1 ATR response replication...
Intratumoural heterogeneity (ITH) is a major cause of cancer-associated lethality. Extensive genomic ITH has previously been reported in clear cell renal carcinoma (ccRCC). Here we address the question whether increases with malignant progression and can hence be exploited as prognostic marker. Unexpectedly, precision quantitative image analysis reveals that degree functional virtually identical between primary ccRCCs lowest stage advanced, metastatic tumours. Functional was found to show...
Prostate-specific membrane antigen (PSMA)–targeting α-radiation therapy (TAT) is an emerging treatment modality for metastatic castration-resistant prostate cancer. There a subgroup of patients with poor response despite sufficient expression PSMA in their tumors. The aim this work was to characterize PSMA-TAT–nonresponding lesions by targeted next-generation sequencing. <b>Methods:</b> Of 60 treated <sup>225</sup>Ac-PSMA-617, we identified 10 who presented tumor uptake PET/CT. We were able...
ABSTRACT Primary human keratinocytes with ectopic expression of high-risk papillomavirus (HPV) E6 and E7 oncoproteins display abnormal centrosome numbers, multipolar mitoses, aneusomy. However, it has not been explored whether these abnormalities can occur in cells containing HPV episomes where is under viral transcriptional control. Here, we demonstrate that genomic instability organotypic raft cultures episomal HPV-16 even at low copy numbers. We conclude DNA, when maintained as an...