A5039930487- Glioma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Brain Metastases and Treatment
- Lung Cancer Treatments and Mutations
- AI in cancer detection
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Meningioma and schwannoma management
- Cervical Cancer and HPV Research
- Skin and Cellular Biology Research
- Radiopharmaceutical Chemistry and Applications
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Cancer-related gene regulation
- Cancer Research and Treatments
- Gene Regulatory Network Analysis
- Cancer Cells and Metastasis
- Gene expression and cancer classification
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
Heidelberg University
2015-2024
Heidelberg University
2009-2024
Universitätsmedizin Göttingen
2023-2024
National Center for Tumor Diseases
2014-2023
University Hospital Heidelberg
2013-2022
Steinbeis University Berlin
2019-2021
Roth and Rau (Germany)
2018
Bellingham Technical College
2016
Steinbeis Foundation
2016
Hamamatsu Medical Center
2012-2013
The prostate-specific membrane antigen (PSMA) targeted positron-emitting-tomography (PET) tracer 68Ga-PSMA-11 shows great promise in the detection of prostate cancer. However, 68Ga has several shortcomings as a radiolabel including short half-life and non-ideal energies, this motivated consideration 18F-labelled analogs. 18F-PSMA-1007 was selected among 18F-PSMA-ligand candidate compounds because it demonstrated high labelling yields, outstanding tumor uptake fast, non-urinary background...
Analysis of tumor-infiltrating lymphocytes (TIL) in primary human colorectal cancer (CRC) by situ immunohistochemical staining supports the hypothesis that adaptive immune response influences course CRC. Specifically, high densities TILs tumor are associated with good prognosis independent other prognostic markers. However, role metastatic CRC lesions is unknown, as their or resistance to conventional chemotherapy. We analyzed association TIL at invasive margin liver metastases chemotherapy...
Abstract Purpose: Tumor infiltrating T lymphocytes in colorectal cancer (CRC) have prognostic impact, but the role of natural killer (NK) cells CRC tissue is unclear. The contribution intratumoral cytokines and chemokines shaping composition inflammatory lymphocytic infiltrate also Experimental Design: In this study, localization densities NK within primary CRC, liver metastases, adenomas, normal tissues were analyzed on whole sections from 112 patients. a subset these patients, most...
Abstract Mutational inactivation of VHL is the earliest genetic event in majority clear cell renal carcinomas (ccRCC), leading to accumulation HIF-1α and HIF-2α transcription factors. While correlative studies human ccRCC functional using lines have implicated as an inhibitor a promoter aggressive tumour behaviours, their roles onset not been functionally addressed. Herein we show autochthonous model that Hif1a essential for formation whereas Hif2a deletion has only minor effects on...
Despite remarkable recent achievements of immunotherapy strategies in cancer treatment, clinical responses remain limited to subsets patients. Predictive markers disease course and response are urgently needed. Recent results have revealed the potential predictive value immune cell phenotype spatial distribution at tumor site, prompting need for multidimensional immunohistochemical analyses tissues. To address this need, we developed a sample-sparing, highly multiplexed immunohistochemistry...
Wound healing is a complex process in which tissue’s individual cells have to be orchestrated an efficient and robust way. We integrated multiplex protein analysis, immunohistochemical whole-slide imaging into novel medium-throughput platform for quantitatively capturing proliferation, differentiation, migration large numbers of organotypic skin cultures comprising epidermis dermis. Using fluorescent time-lag staining, we were able infer source final destination keratinocytes the epidermis....
Abstract Cellular and humoral inflammations play important roles in ischemic brain injury. The effectiveness of immunomodulatory therapies may critically depend on the chosen experimental model. Our purpose was to compare post‐ischemic neuroinflammation among murine permanent transient middle cerebral artery occlusion ( MCAO ) models. Permanent induced by transtemporal electrocoagulation 30 minutes or 90 intraluminal filament C57BL/6 mice. Infiltration leukocyte subpopulations quantified...
Abstract Eight HPV types ( HPV26 , 53, 66, 67, 68, 70, 73 and 82) that are phylogenetically closely related to 12 WHO ‐defined high‐risk HR ) have been rarely but consistently identified as single infections in about 3% of cervical cancer CxCa tissues. Due lack biological data, these referred probable/possible (p) HR‐HPV . To analyse their activity direct comparison types, we selected 55 formalin‐fixed, paraffin‐embedded FFPE tissues harbouring pHR‐HPV (2–13 cases per type) 266 (7–40 from a...
Abstract Background With the advent of primary human papillomavirus testing followed by cytology for cervical cancer screening, visual interpretation slides remains last subjective analysis step and suffers from low sensitivity reproducibility. Methods We developed a cloud-based whole-slide imaging platform with deep-learning classifier p16/Ki-67 dual-stained (DS) trained on biopsy-based gold standards. compared it conventional Pap manual DS in 3 epidemiological studies anal precancers...
High-risk types of human papilloma virus (HPV) are increasingly associated with oropharyngeal squamous cell carcinoma (OPSCC). Strikingly, patients HPV-positive OPSCC highly curable ionizing radiation and have better survival compared HPV-negative patients, but the underlying molecular mechanisms remain poorly understood. We applied an array-based approach to monitor global changes in CpG island hypermethylation between OPSCCs identified a specific pattern differentially methylated regions...
High-level microsatellite instability (MSI-H) in colorectal cancer accounts for about 12% of cancers and is typically associated with a dense infiltration cytotoxic CD8-positive lymphocytes. The role regulatory T cells that may interfere the host's antitumoural immune response MSI-H has not been analysed yet. Using an antibody directed against T-cell marker transcription factor forkhead box P3 (FOXP3), were examined 70 known MSI status (MSI-H, n=37; stable, n=33). In cancers, we found...
Intratumoural heterogeneity (ITH) is a major cause of cancer-associated lethality. Extensive genomic ITH has previously been reported in clear cell renal carcinoma (ccRCC). Here we address the question whether increases with malignant progression and can hence be exploited as prognostic marker. Unexpectedly, precision quantitative image analysis reveals that degree functional virtually identical between primary ccRCCs lowest stage advanced, metastatic tumours. Functional was found to show...
Recurrent gain on chromosome 3q26 encompassing the gene locus for transcription factor SOX2 is a frequent event in human squamous cell carcinoma, including head and neck carcinoma (HNSCC). Numerous studies demonstrated that expression function related to distinct aspects of tumor pathophysiology. However, underlying molecular mechanisms are not well understood, correlation between clinical outcome revealed conflicting data. Transcriptional profiling after silencing HNSCC line identified set...
Lynch syndrome is caused by germline mutations of DNA mismatch repair (MMR) genes, most frequently MLH1 and MSH2. Recently, MMR-deficient crypt foci (MMR-DCF) have been identified as a novel lesion which occurs at high frequency in the intestinal mucosa from mutation carriers, but very rarely progress to cancer. To shed light on molecular alterations clinical associations MMR-DCF, we systematically searched patients for MMR-DCF immunohistochemistry. The lesions were characterised...
A decreasing number of dermatologists and an increasing patients in Western countries have led to a relative lack clinicians providing expert dermatologic care. This, turn, has prolonged wait times for be examined, putting them at risk. Store-and-forward teledermatology improves patient access through asynchronous consultations, reducing obtain consultation. However, live video conferencing as synchronous service is also frequently used by practitioners because it allows immediate...
Abstract Regulatory CD4 + T cells (Treg) prevent tumor clearance by conventional (Tconv) comprising a major obstacle of cancer immune-surveillance. Hitherto, the mechanisms Treg repertoire formation in human cancers remain largely unclear. Here, we analyze clonal origin breast patients using T-Cell Receptor and single-cell transcriptome sequencing. While peripheral blood tumors are clonally distinct, Tconv clones, including tumor-antigen reactive effectors (Teff), detected both compartments....
Undifferentiated cell populations may influence tumor growth in malignant glioma. We investigated potential disruptions the retinoic acid (RA) differentiation pathway that could lead to a loss of capacity, influencing patient prognosis. Expression key molecules belonging RA was analyzed 283 astrocytic gliomas and correlated with proliferation, differentiation, survival. In addition, situ concentrations retinoids were measured tumors, signaling events studied vitro. Unlike other expression...
Viral oncogene RNA expression is regarded as reliable biomarker to identify oropharyngeal squamous cell carcinomas (OPSCC) with active HPV16 involvement. This study addressed whether the profile of cellular proteins p16INK4a, pRb, Cyclin D1 and p53 provide surrogate marker combinations that OPSCC in situations where only formalin-fixed biopsies are available. Protein was analyzed by immunohistochemistry on tissue microarrays created from 188 which DNA status had been established previously...