A5009605367- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Chemotherapy-induced organ toxicity mitigation
- Drug Transport and Resistance Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Microplastics and Plastic Pollution
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- Electrochemical Analysis and Applications
- Biomedical Research and Pathophysiology
- Recycling and Waste Management Techniques
- Acute Kidney Injury Research
- Effects and risks of endocrine disrupting chemicals
- Antimicrobial agents and applications
- Atherosclerosis and Cardiovascular Diseases
- Advanced Materials Characterization Techniques
- Microfluidic and Bio-sensing Technologies
- Heavy Metal Exposure and Toxicity
- Protein Interaction Studies and Fluorescence Analysis
- Identification and Quantification in Food
- Fuel Cells and Related Materials
- Molecular Junctions and Nanostructures
- Nanoparticle-Based Drug Delivery
Universidad Complutense de Madrid
2013-2024
Leiden University
2014-2020
In-Q-Tel
2009
A laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS)-based methodology is presented for Pt, Cu, and Zn bioimaging on whole kidney 3 μm sagittal sections from rats treated with pharmacological doses of cisplatin, which were sacrificed once renal damage had taken place. Pt turned out to accumulate in the cortex corticomedullary junction, corresponding areas where proximal tubule S3 segments (the most sensitive cells cisplatin nephrotoxicity) are located. This demonstrates...
A major area in cancer therapy is the search for protective strategies against cisplatin-induced nephrotoxicity. We investigated effect of cilastatin on injury to renal proximal tubular cells. Cilastatin a specific inhibitor dehydrodipeptidase I (DHP-I), which prevents hydrolysis imipenem and its accumulation tubule. Primary cultures cells were treated with cisplatin (1-30 microM) presence or absence (200 microg/ml). Apoptosis mitochondrial assessed by different techniques. Cisplatin uptake...
The evaluation of the migration ionic silver and nanoparticulated (AgNPs) from antimicrobial plastic packaging to food is crucial ensure its safety. Migration assays were performed on reusable silver-containing polypropylene (PP) containers a silicone baby bottle, using simulants, under conventional or microwave heating repeated use. PP released significant amounts silver, increasing with temperature, contact time, acidity lower crystallinity. Silver in bottle was much lower. Risk assessment...
The interaction of the antitumor drug cisplatin with insulin was studied using a top-down mass spectrometric approach. In vitro incubations were prepared under acidic and physiological conditions at different insulin/cisplatin molar ratios for incubation times. Size exclusion chromatography−inductively coupled plasma spectrometry (SEC−ICPMS) analysis enabled specific detection platinum containing species attributed to binding protein. Further through matrix-assisted laser desorption...
The characterization of the interaction platinum drugs with proteins has been previously performed using bottom-up proteomics approaches (enzymatic digestion followed by MS analysis). Nevertheless, study stability Pt–protein bonds along whole process obviated for moment. Herein suitability treatments implied during enzymatic adducts evaluated, focusing on Pt bonds. Insulin–cisplatin were generated in vitro and separated from unreacted cisplatin HPLC, separation being checked HPLC-ICP-MS....
A hallmark of advanced atherosclerosis is inadequate immunosuppression by regulatory T (Treg) cells inside atherosclerotic lesions. Dyslipidemia has been suggested to alter Treg cell migration affecting the expression specific membrane proteins, thereby decreasing towards Besides cellular metabolism shown be a crucial factor in migration. We aimed determine whether dyslipidemia contributes altered cells, part, metabolism.
A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound Pt detected with high sensitivity LA-ICP-(SF)-MS. Pt-containing spots in-gel digested trypsin, peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence protein separation conditions, staining processing prior laser ablation on bonds preservation have evaluated standard proteins...
Nephrotoxicity is a major limitation to cisplatin antitumor therapies. Cilastatin, an inhibitor of renal dehydropeptidase-I, was recently proposed as promising nephroprotector against toxicity, preventing apoptotic cell death. In this work, cilastatin nephroprotection further investigated in rat model, with focus on its effect 76 lipids altered by cisplatin, including 13 new cisplatin-altered mitochondrial cardiolipin species. Lipid imaging performed MALDI mass spectrometry (MALDI-MSI)...
Acute kidney injury (AKI) is one of the most serious complications cisplatin anticancer therapies. Cilastatin a highly promising nephroprotective agent to eventually enter clinical use, but its biochemical mechanism still not fully understood. We have employed an untargeted metabolomics approach based on capillary electrophoresis mass spectrometry (CE-MS) analysis serum and urine from in vivo rat model, explore metabolic pathways involved cisplatin-induced AKI cilastatin nephroprotection. A...
Proteins in the pulp of olive (Olea europaea) constitute a minor fraction. They have been sparsely studied despite their suggested role oil stability and allergenicity. The analysis protein extract by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) showed major band at 24 kDa that was subjected to tryptic in-gel digestion. Peptide extracts were analyzed MALDI-TOF MS nanoLC-MS/MS. use different search engines enabled assignment number fragmentation spectra peptide...
Nephrotoxicity is a major complication of cisplatin-based chemotherapy, leading to acute kidney injury in ca. 30% patients, with no preventive intervention or treatment available for clinical use. Cilastatin has proved exert nephroprotective effect cisplatin therapies vitro and vivo models, having recently entered trials. A deeper understanding at the molecular level cisplatin-induced renal damage potential protective agents could be key develop successful establish new biomarkers...
The quest for internal standards useful in MALDI imaging studies goes on to get not only lateral distribution but also reliable relative quantitative information. We developed a method based application of matrix and dual allow intra- intersample normalization lipids intensities kidney sections control cisplatin-treated Wistar rats. An inkjet printer was used deposit custom-prepared ink with DHB as matrix, primary lipids-based standard, spiked lanthanide secondary standard. applied different...