A laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS)-based methodology is presented for Pt, Cu, and Zn bioimaging on whole kidney 3 μm sagittal sections from rats treated with pharmacological doses of cisplatin, which were sacrificed once renal damage had taken place. Pt turned out to accumulate in the cortex corticomedullary junction, corresponding areas where proximal tubule S3 segments (the most sensitive cells cisplatin nephrotoxicity) are located. This demonstrates...

Imaging of gadolinium distribution in plants and water flea.

Antitumoral Pt-containing drugs present side effects like nephrotoxicity and ototoxicity. Several systematic experiments have been carried out with Wistar rats treated cisplatin, carboplatin, oxaliplatin to study Pt-drugs accumulation elimination, Pt-biomolecule distribution in the cells cytosols of ear, kidney, liver. Inductively coupled plasma-mass spectrometry (ICP-MS) analysis shows a cisplatin capability between (the highest) carboplatin lowest). The maximum concentration Pt all organs...

In this work, a routinely applicable approach is presented to characterize metal NPs. Individual droplets generated from microdroplet generator (MDG) were merged into an aerosol by pneumatic nebulizer (PN) and introduced ICPMS. The MDG offers high transport efficiency of individual discrete was therefore used establish calibration function for mass quantification NPs which through the PN following single particle procedure as described elsewhere. major advantages such combined configuration...

The judicious use of cis-Pt as an intravenously administrable Pt(II) drug for chemotherapy requires the evaluation its interactions with blood proteins. Therefore, combined modern analytical chemical speciation and proteomics approaches to study these is described here. method involves incubation standard proteins human serum samples. separation conducted by liquid chromatography in anion exchange column (Mono Q). Simultaneous molecular detection UV absorption (280 nm) elemental (195Pt)...

The aim of this work was the purification and identification major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis a protein concentrate recovered from cuttlefish industrial manufacturing effluent. This process consisted on ultrafiltration softening wastewater, with 10 kDa cut-off membrane, followed alcalase retained fraction. Alcalase ACE inhibitors reaching highest activity (IC50 = 76.8 ± 15.2 μg mL−1) after 8 h proteolysis. Sequential hydrolysate...

A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound Pt detected with high sensitivity LA-ICP-(SF)-MS. Pt-containing spots in-gel digested trypsin, peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence protein separation conditions, staining processing prior laser ablation on bonds preservation have evaluated standard proteins...

Abstract Treatment with 1–4 µM As 2 O 3 slightly induced apoptosis in U‐937 human promonocitic leukemia cells. This effect was potentiated by co‐treatment MEK/ERK (PD98059, U0126) and JNK (SP600125, AS601245) inhibitors, but not p38 (SB203580, SB220025) inhibitors. However, no potentiation obtained using lonidamine, doxorubicin, or cisplatin instead of . Apoptosis mitogen‐activated protein kinase (MAPK) inhibitors involved both the intrinsic extrinsic executionary pathways, as demonstrated...

Nephrotoxicity is a major limitation to cisplatin antitumor therapies. Cilastatin, an inhibitor of renal dehydropeptidase-I, was recently proposed as promising nephroprotector against toxicity, preventing apoptotic cell death. In this work, cilastatin nephroprotection further investigated in rat model, with focus on its effect 76 lipids altered by cisplatin, including 13 new cisplatin-altered mitochondrial cardiolipin species. Lipid imaging performed MALDI mass spectrometry (MALDI-MSI)...

Most of cisplatin's citotoxic properties are due to the interaction drug with DNA. However, other biological molecules present in cell cytosol, such as MT (metallothionein) and GSH (glutathione), potential targets for cisplatin have been related its side-effects or cellular resistance mechanisms drug. Experiments simulating physiological conditions performed study specific metabolites which interact characterize different drug–biomolecule adducts over time. A combination size exclusion...

Sensitive, accurate and fast absolute quantification of intact proteins is reported using metal coded affinity tags (MeCATs) combined with gel electrophoresis (GE) separation, inductively coupled plasma mass spectrometry (ICP-MS) detection label-specific isotope dilution analysis quantification. Two different approaches were investigated concerning the introduction sample into ICP-MS, mineralization direct laser ablation (LA) spot. Three major advantages are remarkable in proposed method....

A robust ICPMS-based method is introduced to obtain relative and absolute quantification of sulfenic acid (SA) in peptides proteins. new metal-containing reagent (Ln-DOTA-Dimedone) devised react specifically with SA has been developed. The lanthanide-containing metal-coded affinity tag (Ln-MeCAT) was used quantify thiol residues. We presented two approaches which allow the parallel consecutive determination thiols peptide protein samples. high sensitivity, structure-independent signal,...

Metal‐Coded Affinity Tags (MeCAT) reagents were devised for the absolute quantification of labeled proteins and peptides using inductively coupled plasma mass spectrometry (ICP‐MS). After recent publication approaches digested proteins, this work presents a multidimensional strategy application MeCAT to samples which require higher chromatographic resolution. Two‐dimensional separations based on strong cation exchange (SCX) reversed‐phase (RP) chromatography, used lysozyme, bovine serum...

Sample preparation continues being a key factor to obtain fast and reliable quantification of Hg species. Assisted procedures enhance the efficiency reduce extraction time; however, collateral species transformations have been observed. Moreover, differential interconversions observed even between similar matrixes, which introduce an important uncertainty for real sample analysis. Trying minimize transformations, we tested soft ultrasound-assisted procedure. Species evaluated using double...

In this work we present a methodology to measure the complex adduct spectrum caused by interaction of Cisplatin with DNA. By using an optimized DNA digestion procedure were able show that in vivo duplex is much more than described so far. For first time high abundance interstrand adducts has been detected HPLC/ESI-MS. These could play key role repair mechanisms and development cellular resistance Cisplatin. species-unspecific isotope dilution analysis HPLC/ICP-MS measurements, study kinetics...