A5029781695- Chemotherapy-induced organ toxicity mitigation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Analytical chemistry methods development
- Cardiovascular and exercise physiology
- Acute Kidney Injury Research
- Pharmacological Effects and Toxicity Studies
- Advanced Proteomics Techniques and Applications
- Renal Diseases and Glomerulopathies
- Metabolomics and Mass Spectrometry Studies
- Biomedical Research and Pathophysiology
- Influenza Virus Research Studies
- Exercise and Physiological Responses
- Chronic Kidney Disease and Diabetes
- Neuroendocrine Tumor Research Advances
- Liver Disease and Transplantation
- Archaeological and Geological Studies
- Electrolyte and hormonal disorders
- Renal function and acid-base balance
- Drug Transport and Resistance Mechanisms
- Mitochondrial Function and Pathology
- Pneumonia and Respiratory Infections
- Cancer Treatment and Pharmacology
- Mass Spectrometry Techniques and Applications
- Vaccine Coverage and Hesitancy
- Nanoparticle-Based Drug Delivery
Universidad Complutense de Madrid
2011-2024
Hospital General Universitario Gregorio Marañón
2015-2024
Petrobras (Brazil)
2024
Hospital Clínico San Carlos
2002-2022
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2022
Comunidad de Madrid
2018
Humboldt-Universität zu Berlin
2018
Brigham and Women's Hospital
2017
Harvard University
2017
Universidad Autónoma de Madrid
2005-2012
A laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS)-based methodology is presented for Pt, Cu, and Zn bioimaging on whole kidney 3 μm sagittal sections from rats treated with pharmacological doses of cisplatin, which were sacrificed once renal damage had taken place. Pt turned out to accumulate in the cortex corticomedullary junction, corresponding areas where proximal tubule S3 segments (the most sensitive cells cisplatin nephrotoxicity) are located. This demonstrates...
Contrast-Induced Acute Kidney Injury (CI-AKI) remains a frequent iatrogenic condition since radiological procedures using intra-vascular iodinated contrast media (CM) are being widely administered for diagnostic and therapeutic purposes. Despite the improvement of medical healthcare system worldwide, CI-AKI is still associated with direct short-term indirect long-term outcomes including increased morbidity mortality, especially in patients underlying pre-existing renal function impairment,...
Cisplatin is a potent chemotherapeutic drug whose nephrotoxic effect major complication and dose-limiting factor for antitumoral therapy. There much evidence that inflammation contributes to the pathogenesis of cisplatin-induced nephrotoxicity. We found cilastatin, renal dehydropeptidase-I inhibitor, has protective effects in vitro vivo against damage by inhibiting apoptosis oxidation. Here, we investigated potential use cilastatin protect kidney injury rats. Male Wistar rats were divided...
Abstract Atherosclerosis is a chronic disease that affects medium and large arteries. This process originates from the interaction between cells of arterial wall, lipoproteins inflammatory cells, leading to development complex lesions or plaques protrude into lumen. Plaque rupture thrombosis result in acute clinical complications such as myocardial infarction stroke. Owing heterogeneous cellular composition plaques, proteomic analysis whole lesion not appropriate. Therefore, we have studied...
IgG Fc receptors (FcγRs) play a role in activating the immune system and maintaining peripheral tolerance, but their atherosclerosis is unknown. We generated double-knockout (DKO) mice by crossing apolipoprotein E–deficient (apoE −/− ) with FcγR γ chain–deficient (γ ). The size of atherosclerotic lesions along aorta was approximately 50% lower DKO compared apoE control mice, without differences serum lipid levels. macrophage T-cell content were reduced 49±6% 56±8%, respectively, lesions....
A major area in cancer therapy is the search for protective strategies against cisplatin-induced nephrotoxicity. We investigated effect of cilastatin on injury to renal proximal tubular cells. Cilastatin a specific inhibitor dehydrodipeptidase I (DHP-I), which prevents hydrolysis imipenem and its accumulation tubule. Primary cultures cells were treated with cisplatin (1-30 microM) presence or absence (200 microg/ml). Apoptosis mitochondrial assessed by different techniques. Cisplatin uptake...
Vancomycin is a very effective antibiotic for treatment of severe infections. However, its use in clinical practice limited by nephrotoxicity. Cilastatin dehydropeptidase I inhibitor that acts on the brush border membrane proximal tubule to prevent accumulation imipenem and toxicity. The aim this study was investigate potential protective effect cilastatin vancomycin-induced apoptosis toxicity cultured renal tubular epithelial cells (RPTECs). Porcine RPTECs were presence vancomycin with...
<h3>Background</h3> Exhausting exercise reduces the mitochondrial DNA (mtDNA) content in skeletal muscle of healthy subjects due to oxidative damage. Since patients with chronic obstructive pulmonary disease (COPD) suffer enhanced stress during exercise, it was hypothesised that mtDNA will be further reduced. <h3>Objective</h3> To investigate effects above and below lactate threshold (LT) on COPD. <h3>Methods</h3> Eleven COPD (67±8 years; forced expiratory volume 1 s (FEV<sub>1</sub>)...
Exercise triggers skeletal muscle oxidative stress in patients with chronic obstructive pulmonary disease (COPD). The objective of this research was to study the specific sites reactive oxygen species (ROS) production mitochondria isolated from COPD and its relationship local induced by exercise. Vastus lateralis biopsies were obtained 16 (66 ± 10 yr; FEV(1), 54 12% ref) 14 control subjects normal lung function who required surgery because cancer (65 7 91 14% at rest after In these we...
Gentamicin is a used antibiotic that causes nephrotoxicity in 10–20% of treatment periods, which limits its use considerably. Our results have shown cilastatin may be promising therapeutic alternative toxin-induced acute kidney injury (AKI). Here, we investigated potential as nephroprotector against gentamicin-induced AKI vitro and vivo. Porcine renal cells rats were treated with gentamicin and/or cilastatin. In vivo was analyzed by measuring biochemical markers morphology. Different...
Skeletal muscle dysfunction (SMD) is frequent in patients with chronic obstructive pulmonary disease (COPD). Mitochondrial abnormalities appear to play a role the pathogenesis of SMD. The mitochondrion permeability transition pore (MPTP) facilitates leakage mitochondrial matrix constituents, such as cytochrome c (cyto-c), and triggers apoptosis, known occur skeletal COPD. Our objective was study MPTP kinetics cyto-c release mitochondria Mitochondria were isolated from vastus lateralis (VL),...
A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound Pt detected with high sensitivity LA-ICP-(SF)-MS. Pt-containing spots in-gel digested trypsin, peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence protein separation conditions, staining processing prior laser ablation on bonds preservation have evaluated standard proteins...
Nephrotoxicity is a major limitation to cisplatin antitumor therapies. Cilastatin, an inhibitor of renal dehydropeptidase-I, was recently proposed as promising nephroprotector against toxicity, preventing apoptotic cell death. In this work, cilastatin nephroprotection further investigated in rat model, with focus on its effect 76 lipids altered by cisplatin, including 13 new cisplatin-altered mitochondrial cardiolipin species. Lipid imaging performed MALDI mass spectrometry (MALDI-MSI)...
Contrast-Induced Acute Kidney Injury (CI-AKI) remains a frequent iatrogenic condition since radiological procedures using intra-vascular iodinated contrast media (CM) are being widely administered for diagnostic and therapeutic purposes. Despite the improvement of medical healthcare system worldwide, CI-AKI is still associated with direct short-term indirect long-term outcomes including increased morbidity mortality, specially, in patients under-lying pre-existing renal function impairment,...
Glaucoma is a neurodegenerative disease that causes blindness. In this study, we aimed to evaluate the protective role of cilastatin (CIL), generally used in treatment nephropathologies associated with inflammation, an experimental mouse model based on unilateral (left) laser-induced ocular hypertension (OHT). Male Swiss mice were administered CIL daily (300 mg/kg, i.p.) two days before OHT surgery until sacrifice 3 or 7 later. Intraocular Pressure (IOP), as well retinal ganglion cell (RGC)...
ABSTRACT Background Proteinuria is not only a biomarker of chronic kidney disease (CKD) but also driver CKD progression. The aim this study was to evaluate serum and urinary tubular biomarkers in patients with biopsied proteinuric correlate them histology outcomes. Methods A single-center retrospective conducted on cohort 156 from January 2016 December 2021. following were analyzed the day biopsy: beta 2 microglobulin (β2-mcg), alpha 1 (α1-mcg), neutrophil gelatinase-associated lipocalin...
Acute kidney injury (AKI) is one of the most serious complications cisplatin anticancer therapies. Cilastatin a highly promising nephroprotective agent to eventually enter clinical use, but its biochemical mechanism still not fully understood. We have employed an untargeted metabolomics approach based on capillary electrophoresis mass spectrometry (CE-MS) analysis serum and urine from in vivo rat model, explore metabolic pathways involved cisplatin-induced AKI cilastatin nephroprotection. A...