Abstract Secreted proteins determine a range of cellular functionalities correlated with human health and disease progression. Because cell heterogeneity, it is essential to measure low abundant protein secretions from individual cells single‐cell activities. In this study, an integrated platform consisting smart hydrogel immunosensors for the sensitive detection developed. A single microparticles are encapsulated within droplet. After incubation, target secreted captured in particle...

A membrane based microfluidic device integrated with a surface plasmon resonance sensor was used for interferon-gamma detection, the availability of quantitative analysis.

A simple design of a label-free colorimetric assay for IgE was demonstrated based on structure-switching aptamer with low detection limit 0.2 nM and high selectivity.

In the context of tumor analysis, implementation precision medicine requires on-time clinical measurements, which rapid large-scale single-cell screening that obtains cell population distributions and functions in tumors to determine disease progression for therapeutics. this study, a high-throughput (HTS) platform integrating optical fluorescence detectors computational method was developed as droplet-based microfluidic flow cytometer (Droplet-μFC) comprehensively analyze multiple...

Abstract Immunoassays are an important tool in various bioanalytical settings, such as clinical diagnostics, biopharmaceutical analysis, environmental monitoring, and food testing. An enzyme‐linked immunosorbent assay (ELISA) is usually used to amplify immunoassay signals; however, it requires labor‐intensive time‐consuming procedures, which hinders its application rapid cytokine detection. In this study, a nano‐in‐micro composite system, where immunosensing polystyrene beads (≈320 nm)...

Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor the rapid of nucleic acids. The sensor successfully achieved loop-mediated isothermal amplification hepatitis virus work. comprised nanodisc array Bst polymerases conjugated on rough surface nanodisc. provided hot spots to enhance fluorescence signal. DNA was detected by conducting modified with resonance energy transfer...

Cell nucleocytoplasmic profiles of microRNAs (miRNAs) are critical to determining a single cell's essential functionalities, such as cellular transcription, nucleus export and degradation, which gives comprehensive view processes. Despite the importance addressing heterogeneity, challenge high-throughput screening remains. Although droplet-based approach was developed for single-cell miRNA assays, quantifying with high sensitivity indicate heterogeneity In this study, nanoplasmon-enhanced...

A tip nanobiosensor for monitoring DNA replication was presented. The effects of excitation power and polarization on tip-enhanced fluorescence (TEF) were assessed with the immersed in fluorescein isothiocyanate solution first. photon count rose average fivefold radially polarized illumination at 50 mW. We then used polymerase-functionalized tips loop-mediated isothermal amplification Hepatitis C virus cDNA. amplicon-SYBR® Green I complex detected compared to real-time amplification. signals...

With the continuous advancement of stage technology, modern designers are able to operate various elements art at a more sophisticated level, achieve complex effect previews, and optimize visual impact performances. This technological not only improves rapid accurate communication design intent, but also gives highly personalized innovative feature. The development trajectory shows infinite possibilities integration reveals leap from traditional handicrafts machinery, then today s digital...

A calculation based on surface plasmon coupling condition and Maxwell-Garnett equation was performed for predicting the angle shift thin film thickness in scanning microscopy (SSPM). The refractive index sensitivity lateral resolution of an SSPM system also investigated. limit detection 0.01°, quantification 0.03°, around 0.12° per nm ZnO when less than 22.6 nm. Two partially connected Au nano-discs with a center-to-center distance 1.1 μm could be identified as two peaks. applied to image...

Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (∼100 nm) an atomic force microscope (AFM) tip and performed LAMP hepatitis B virus (HBV). By using this tip-based reaction, replicated HBV can be directly imaged 400∼500 nm spots two-photon excitation microscopy.

ABSTRACT Owing to the highly efficient two‐photon fluorescence of gold nanorods and very short lifetime compared with rotational correlation time, rotation diffusion a single nanorod can be easily observed by spectroscopy (TP‐FCS). This property, along previous successful use as contrast agent in imaging, suggests potential application TP‐FCS well. Although FCS measurement becomes probes, amplitude temporal decay measured functions depend critically on excitation power. Here, we investigate...

This study demonstrates the advantage of Dip-Pen Nanolithography (DPN) as a research and design tool for metal nano-structure fabrications. We two different gold nano-structures, which are fabricated by DPN etching method with temperature humidity control. The plasmon resonance frequencies both structures measured dark field scattering spectroscopy. Our results show that control, is highly potential in developing photonic circuit, solar cell biomedical devices due to rapid fabrication cost...

We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and C (HCV) on the apex (50~100 nm) metallic tips coated with Bst polymerases. The SYBR green molecules binding to new formed HBV DNA inside optical near fields were excited by two-photon fluorescence microscopy, directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented this manuscript.