Diarrhea in piglets directly affects commercial swine production. The disease results from the interaction of pathogens with host immune system and is also affected by management procedures. Several pathogenic agents such as Campylobacter spp., Clostridium perfringens, Escherichia coli, Salmonella group A rotavirus (RV-A), coronaviruses (transmissible gastroenteritis virus; porcine epidemic diarrhea virus), well nematode protozoan parasites, can be associated cases.All bacterial, viral,...

The extent of knowledge regarding the diversity globally distributed Ehrlichia canis strains has been limited to information gained from a few evolutionarily conserved genes. In this study, E. United States (strain Jake [US]), Brazil São Paulo [BR]), and Israel 611 [IS] Ranana [IS-R]) were used examine antigenic genetic diversities four well-characterized major immunoreactive protein genes/proteins. gp36 gp200 most divergent genes, nucleotide substitutions in tandem repeat region IS strain,...

Human monocytotropic ehrlichiosis is an emerging life-threatening zoonosis caused by obligately intracellular bacterium, Ehrlichia chaffeensis. E. chaffeensis transmitted the lone star tick, Amblyomma americanum, and replicates in mononuclear phagocytes mammalian hosts. Differences transcriptome arthropod hosts are unknown. Thus, we determined host-specific gene expression human monocyte (THP-1) Ixodes tick cell lines (AAE2 ISE6) using a whole genome microarray.The majority (∼80%) of genes...

This paper aimed to determine the excretion period of B19 vaccine strain during a complete reproductive cycle (from estrus synchronization, artificial insemination, pregnancy and until 30 days after parturition) dairy cows from 3 9 years old that were previously vaccinated 8 months. Three groups monitored with monthly milk urine collection 12 months: G1 seven 4 old; G2 three 5 6 G3 four 7 old. Urine samples submitted bacteriological culture PCR reactions for detection Brucella spp....

Caseous lymphadenitis is a chronic infectious disease caused by Corynebacterium pseudotuberculosis , which bacterium responsible for great number of economic losses on goat and sheep production. It characterized the formation abscesses in superficial lymph nodes internal organs nodes. This study aimed at determining agreement between microbiological culture PCR identification C. samples collected from animals slaughterhouses that presented node enlargement field conditions. From 202 analyzed...

ABSTRACT The objectives of the present trial were to evaluate reproductive parameters in a Holstein bovine herd affected by leptospirosis during outbreak abortion and stillborn fetus treated with streptomycin sulfate. Animals divided 2 groups 10 cows each: G1, sulfate 25 mg/kg/lw for 3 days IM route; and; G2, untreated control. Serum samples collected on onset abortions after that every 15 or 30 until completing 270 days. Samples analyzed means MAT microscopic agglutination test showed sera...

The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These be found in water, food and the intestinal tract chickens. This study investigated presence jejuni coli chicken abattoirs São Paulo State, Brazil. A total 288 samples feces, feathers, scald evisceration chiller rinse water eviscerated, not eviscerated chilled carcasses were collected six abattoirs. Polymerase Chain Reaction (PCR) was performed spp.-positive...

Postmortem examination of a Boer buck that died peracutely revealed bowel and liver diffusely congested edematous. Kidney was apparently Clostridium perfringens type A isolated from D kidney. Microscopic large areas necrosis in the renal cortex medulla (pulpy kidney disease), hyperemia centrilobular liver, small-intestine wall, pulmonary edema congestion, intense cerebellum, brain.

A total of 192 samples illegal cheese from different regions the states São Paulo and Minas Gerais, Brazil, were analyzed for isolation detection Brucella spp. DNA by means microbiological culture polymerase chain reaction (PCR), respectively. Samples that yielded positive results submitted to analysis occurrence abortus (biovars 1, 2 e 4), as well differentiation in B19 vaccinal strain or field using PCR. Although microorganism was not isolated any sample, PCR detected 37 (19.27%)...

Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, has the man as end point of its epidemiological chain. diagnosis in primates more difficult than other animal species, clinical signs lesions are less evident antibody response detected only for short periods. The aim this article was to describe detection Leptospira spp using polymerase chain reaction (PCR), samples from one captive black-capped Capuchin monkey (Cebus apella), which presented...

The objective of the present trial was to characterize genetically strains Campylobacter jejuni subsp. isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars poultry). A total 828 different samples (feces, carcass, aborted fetus hysterectomized uterus) were analysed by means routine bacteriological methods, 36 C. isolated. Thirty human fecal origin obtained in clinical analysis laboratories city São Paulo. 66 submitted genetic characterization. Primers...

Mycobacterium was verified in animals from a Brazilian dairy herd, total of 42 samples 30 cows were submitted to culture and the isolated strains analyzed by two polymerase chain reaction (PCR), first specific for species belonging complex (MTBC) other differentiating M. tuberculosis bovis. Twenty seven (64.3%) 18 (60%) positive mycobacteria culture, including 15 retrofaryngeal lymphnodes (55.5%), 9 prescapular (33.3%), 2 lungs (7.4%), 1 liver (3.7%). All colonies confirmed PCR contain MTBC...

Toxigenic types of Clostridium perfringens are significant causative agents enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause enterotoxemia lambs, calves and rabbits. We report herein the typing 23 C. strains, by polymerase chain reaction (PCR) technique, isolated from small intestine samples bovines that have died suddenly, after manifesting or not neurological disorders. Two strains (8.7%) were identified E, two D remainder A (82.6%)....

The objectives of the present study were subtyping Campylobacter jejuni subsp. strains obtained from humans and different animal species using PCR-RFLP, detection, by means same technique, related to serotype PEN O19:LIO 7, main C. linked Guillain-Barré Syndrome (GBS). Seventy isolated human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material goats (n=2) poultry carcasses (n=12), all collected in state São Paulo, subtyped PCR-RFLP fla A gene, restriction...

C. chauvoei presence was detected by means of polymerase chain reaction (PCR) from supernatant culture in cooked meat medium liver, muscle and metatarsian bone marrow samples seven calves with blackleg symptoms.The isolation under anaerobic conditions one sample revealed Clostridium perfringens pure culture.

Foram coletados 100 suabes retais e de carcaças bovinas em matadouros do estado São Paulo, um total 326 estirpes E. coli foram identificadas, sendo 163 amostras carcaça. Todos os isolados submetidos à PCR para detecção dos genes das toxinas Stx1 Stx2 identificados como não-O157 fenotipados pelo teste da citotoxicidade células Vero. Das 26 que apresentaram apenas o gene stx1, 56 stx2 30 ambos genes, 17 (65,4%), 42 (75%) 22 (73,3%), respectivamente, positivas ao citotoxicidade. Não houve...

Isolaram-se estirpes de Campylobacter spp. em amostras carcaças (n=65), fezes (n=65) e linfonodos mesentéricos suínos abatidos frigoríficos do estado São Paulo detectaram, pela técnica da Multiplex-PCR, a presença complexo genes cdt, responsáveis expressão fator virulência toxina CDT. Do total 195 origem suína, foi isolado 31 (15,9%), sendo 29 (93,6%) suabe retal, 1/65 (3,2%) carcaça um linfonodo. Vinte oito C. coli foram positivas para detecção dos três jejuni negativas desses genes. Foi...

Doença bacteriana zoonótica, a campilobacteriose é responsável mundialmente por frequentes casos de gastroenterite humana. Campylobacter spp. apresenta fator virulência associado à diarreia, denominado toxina citoletal distensiva (CDT), sendo codificado pelos genes do complexo cdt. Os objetivos presente estudo foram: 1) isolar e identificar estirpes 102 suabes carcaças retais ovinos (Ovis aries) sete amostras água dos efluentes, antes depois tratamento desinfecção abatedouro localizado no...