Equilibration of oocytes with cryoprotectants is a prerequisite low temperature storage. However, cryoprotectant exposure may induce damage via osmotic stress. Knowledge cell membrane permeability characteristics and their dependence would facilitate the design cryopreservation protocols in which stress minimized incidence intracellular freezing reduced. To obtain such data, volume change donated human following to was measured at variety temperatures. After removal cumulus cells, each...

Brazilian family farming comprises production units that represent approximately 86.40% of agricultural establishments, playing a significant national role in socio-environmental and economic sectors. The state São Paulo is known for its rural urban development, including settlements such as Pontal do Paranapanema. This sector requires attention due to the emergence infectious diseases, leptospirosis, widespread zoonotic disease causes losses productivity. study aimed analyze prevalence...

This study has detected BPV-1 DNA sequences in wart, blood and plasma samples collected from animals affected by papillomatosis, suggesting viral presence inside the cell. We sellected an animal which we could detect blood, placenta amniotic liquid her offspring presented sample immediately after birth. These results show a possible vertical transmission of BPV-1.

Mining dam disasters contribute to the contamination of aquatic environments, impacting associated ecosystems and wildlife. A multidrug-resistant Escherichia coli strain (B2C) was isolated from a river water sample in Brazil after Mariana mining disaster. The genome sequenced using Illumina MiSeq platform, de novo assembled Unicycler. Resistome, virulome, plasmidome were predicted bioinformatics tools. Data analysis revealed that E. B2C belonged sequence type ST219 phylogroup Strikingly,...

Background Bovine respiratory disease ( BRD ) is an important problem in cattle production that responsible for economic losses dairy herds. Mycoplasma spp. are described as etiological agent of . Hypothesis To evaluate the occurrence most mycoplasmas lower tract healthy and relationship to clinical signs Animals Sixty young were classified (n = 32) or showing 28). Methods Tracheal lavage samples collected added tubes containing Hayflick media. identified by presence “fried egg” like...

ABSTRACT: Bovine respiratory disease (BRD) is considered the major cause of economic losses in dairy and beef cattle production. The study aimed to detect most important bacteria related tracheobronchial fluid samples healthy calves with clinical signs BRD Brazilian rural settlements. Hundred forty-one mongrel were randomly selected from 42 family farm herds Physical examination was performed classified as (n=100) (n=41). Tracheobronchial collected. Isolation molecular detection Mycoplasma...

Caseous lymphadenitis is a chronic infectious disease caused by Corynebacterium pseudotuberculosis , which bacterium responsible for great number of economic losses on goat and sheep production. It characterized the formation abscesses in superficial lymph nodes internal organs nodes. This study aimed at determining agreement between microbiological culture PCR identification C. samples collected from animals slaughterhouses that presented node enlargement field conditions. From 202 analyzed...

This study aimed to determine the occurrence of Mannheimiahaemolytica, Pasteurella multocida and Mycoplasma spp., in relation clinical signs respiratory disease. Tracheobronchial lavage samples were collected from 96 (healthy unhealthy) cattle State São Paulo, Brazil. spp. (12.5 %) Pasteurellamultocida (15.50 most prevalent species. Bacillus sp., Staphylococcus Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa pneumoniae also isolated. Mollicutes (70.83 %), Mycoplasmabovis (2.94...

The use of heavy metals in economic and social development can create an accumulation toxic waste the environment. High concentrations damage human animal health, lead to antibiotic resistance, possibly change bovine microbiota. It is important investigate influence food systems determine potential harmful effects environmental metal contamination on health. Because a mining dam rupture, 43 million cubic meters iron ore flowed into Doce river basin surrounding Mariana City, Brazil, 2015....

ABSTRACT: Respiratory diseases are among the most important in sheep flocks. Herein was studied bacterial etiology of respiratory disease and clinical signs 99 female male breed states São Paulo (SP) Rio de Janeiro (RJ), Brazil. After physical examination animals, tracheobronchial flushing samples were obtained. The usual bacteria Mycoplasma spp. searched, as well their association with status disease. main observed were: tachypnea (75%), increase rectal temperature (09.4%),...

O presente estudo estabelece valores de referência dos teores séricos proteínas totais, albumina, AST e GGT em ovinos da raça santa Inês avalia a interferência fatores sexuais etários sobre estas variáveis. Foram colhidas amostras sangue 161 ovelhas. resultado encontrado para os padrões foram 5,86 + 0,96 g/dL 2,33+0,43 63,25+27,52 U/L 28,45+14,53 GGT. A análise resultados permite afirmar que referentes à concentração sérica albumina atividade sofrem influência etários, somente etários. Não...

RESUMO Com o objetivo de estabeler os valores referência parâmetros bioquímicos que avaliem a função renal, através da determinação dos teores séricos uréia e creatinina, em bovinos raça Jersey, criados no Estado São Paulo, bem como avaliar influência fatores etários, sexuais infecção pelo vírus leucose sobre referidos bioquímicos, foram colhidas examinadas amostras soro sanguíneo 171 animais, sendo 106 fêmeas 18 machos, considerados clinicamente sadios não reagentes ao antígeno...

RESUMO Uma cabra da raça Saanem, com 3 anos, foi atendida no Hospital Veterinário Faculdade de Medicina Veterinária e Zootecnia Universidade São Paulo, histórico emagrecimento progressivo, doença respiratória não responsiva a antibioticoterapia convencional. O animal apresentava perda peso, aumento linfonodo submandibular esquerdo sintomas broncopneumonia. Tendo sido considerado positivo teste cervical comparativo sacrificado à necropsia foram observadas lesões sugestivas tuberculose nos...

In a clinical survey of caprine arthritis-encephalitis in 14 naturally infected herds the State São Paulo, Brazil, 17.1% (64/374) seroreagents presented articular form disease and 6.6% (17/249) seroreagent females mammary disease.

ABSTRACT: Bovine respiratory disease (BRD) is responsible for economic losses in cattle production. Viruses are categorized as primary etiological agents. The aims of this study were to evaluate the presence antibodies against bovine viral diarrhea virus (BVDV), herpes type 1 (BoHV-1), and syncytial (BRSV) healthy BRD calves from family farming relation clinical signs BRD. Hundred forty-five randomly selected physical examination was performed. Only 123 animals classified calves. Antibodies...

RESUMO: Objetivou-se avaliar a ocorrência, sinais clínicos e fatores de risco associados soropositividade para Toxoplasma gondii Neospora caninum em ovinos. Foram utilizados 294 animais com histórico distúrbios reprodutivos 28 fazendas do estado São Paulo, Brasil, diagnosticados através da imunofluorescência indireta (1:64 1:50). A ocorrência T. foi 29,9% (88/294) N. 18% (53/294), sendo 3,7% (11/294) dos ovinos soropositivos ambos. Observou-se maiores chances à infecção pelo gondii: mestiços...