Abstract Multi-modal imaging is essential for advancing our understanding of brain function and unraveling pathophysiological processes underlying neurological psychiatric disorders. Magnetic resonance (MR) optoacoustic (OA) have been shown to provide highly complementary contrasts capabilities preclinical neuroimaging. True integration between these modalities can thus offer unprecedented studying the rodent in action. We report on a hybrid magnetic tomography (MROT) system concurrent...

Abstract Imaging techniques capable of visualizing the nervous and vascular systems are essential for uncovering fundamental mechanisms underlying brain functions. To enable visualization both at microscale with a streamlined imaging setup, dual‐modal two‐photon fluorescence optoacoustic microscopy (TPOAM) is developed neuronal calcium activity concurrently label‐free hemodynamic detection. TPOAM achieves sub‐micron lateral resolution real‐time multi‐plane capability temporal down to 100 ms,...

Background: Microcirculation is essential for skin homeostasis and repair. A variety of growth factors have been identified as important regulators wound healing. However, direct observation longitudinal monitoring remodeling in an unperturbed vivo environment remains challenging. Methods: We report on non-invasive imaging the healing process transgenic mice overexpressing vascular endothelial factor (VEGF-A) keratinocytes by means large-scale optoacoustic microscopy (LSOM). This rapid,...

Abstract Functional magnetic resonance imaging (fMRI) has massively contributed to the understanding of mammalian brain function. However, origin and interpretation blood oxygen level‐dependent (BOLD) signals retrieved by fMRI remain highly disputed. This article reports on development a fully hybridized system enabling concurrent functional optoacoustic tomography (MROT) measurements stimulus‐evoked brain‐wide sensory responses in mice. The complementary angiographic soft tissue contrasts...

Abstract Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping biodistribution in three dimensions. We introduce a volumetric wide-field microscopy based on optical astigmatism combined with source localization, covering 5.6×5.6×0.6 mm 3 volume. Two alternative configurations are proposed exploiting multifocal illumination...

An ultrasound detection scheme based on a transparent polyvinylidene-fluoride indium-tin-oxide (PVDF-ITO) piezoelectric film is developed for ultrawideband sensitive of optoacoustic (OA) signals down to noise equivalent pressure (NEP) 8.4 Pa over an effective bandwidth extending beyond 30 MHz. The high signal-to-noise ratio and low performance are facilitated by employing two-stage amplifier structure. PVDF-ITO detector directly mounted commercial numerical aperture objective lens scanning...

BACKGROUND: Ischemic stroke is a common cause of death worldwide and main morbidity. Presently, laser speckle contrast imaging, x-ray computed tomography, magnetic resonance imaging are the mainstay for diagnosis therapeutic monitoring in preclinical studies. These modalities often limited terms their ability to map brain perfusion with sufficient spatial temporal resolution, thus calling development new techniques featuring rapid speed, cost-effectiveness, ease use. METHODS: We report on...

A wideband sensitive needle ultrasound sensor based on a polarized PVDF-TrFE copolymer piezoelectric film has been developed, which is capable of providing noise equivalent pressure 14 Pa and uniform frequency response ranging from 1 to 25 MHz. Its high sensitivity (1.6 μV / Pa) compact size were achieved by capitalizing the large electromechanical coupling coefficient minimizing parasitic capacitance in two-stage amplifier structure. The detection newly designed outperformed commercially...

Skull bone development is a dynamic and well-coordinated process playing key role in maturation maintenance of the marrow (BM), fracture healing, progression diseases such as osteoarthritis or osteoporosis. At present, transformation growing (osteogenesis) well its vascularization (angiogenesis) remain largely unexplored due to lack suitable vivo imaging techniques capable noninvasive visualization whole developing calvaria at capillary-level resolution. We present longitudinal study on...

Chronic, non-healing wounds constitute a major health problem, and the current therapeutic options are limited. Therefore, pharmaceuticals that can be locally applied to complicated urgently needed. Such treatments should directly target underlying causes, which include diabetes mellitus, chronic local pressure and/or vascular insufficiency. A common consequence of these disorders is impaired wound angiogenesis. Here, we investigated effect topical application nitric oxide-releasing...

This study investigated the correlation between periprostatic fat thickness (PPFT) measured on magnetic resonance imaging and lower urinary tract symptoms, erectile function, benign prostatic hyperplasia (BPH) progression. A total of 286 treatment-naive men diagnosed with BPH in our department March 2017 February 2019 were included. Patients divided into two groups according to median value PPFT: high (PPFT >4.35 mm) PPFT group low <4.35 group. After initial evaluation, all patients...

Abstract C 22 H 19 NO 6 , monoclinic, P 2 1 / n (no. 14), a = 9.055(3) Å, b 17.601(6) c 11.817(4) β 91.361(5)°, V 1882.8(11) Å 3 Z 4, R gt ( F ) 0.0368, wR ref 0.1038, T 296(2) K.

Short-pulsed solid-state lasers (SSLs) are the most commonly employed light sources in optoacoustic imaging applications. However, their bulky size hinders compact and portable system implementations. Here we developed a laser diode (LD)-based mesoscopy (CoLD-OAM) scanner that employs fiber-coupled source with 46 × 43 11 mm dimensions. CoLD-OAM features scalable excitation pulse width 30–200 ns range, high energies up to 6 µJ, excellent pulse-to-pulse energy stability of 0.42%. Real-time...

Given its direct relationship to tissue metabolism and various pathological processes, 3D mapping of blood oxygen saturation (sO2) is essential for advancing our knowledge on delivery tissues evaluating therapeutic efficacy. Optoacoustic microscopy has enabled label-free estimation sO2 values by exploiting the spectrally distinctive absorption hemoglobin in oxygenated deoxygenated forms. However, quantitative distribution over large heterogenous regions commonly hindered due strong spatial...

We observed and quantified VEGF-driven enhancement of dermal vascularization in healing wounds by LSOM. The imaging approach can supplement or replace the cumbersome histological procedures to study skin regeneration its unperturbed vivo environment.

Objective To summarize the clinical experience of comprehensive management critical newborns with transposition great arteries(TGA)by neonatologists and cardiac surgeons. Methods Seventeen TGA rescued by combined efforts pediatric surgeons from Oct.2010 to Oct.2012 were retrospectively analyzed. Results Of 17 newborns(14 cases male, 3 female), 9 ventricular septal defect(TGA/VSD), 8 intact defect(TGA/IVS). The babies aged 1-45 days, weighed 2.7-4.6 kg at operation.Of TGA/VSD...

Abstract Ischemic stroke is a common cause of death worldwide and main morbidity. Presently, computed tomography magnetic resonance imaging are the mainstay for diagnosis therapeutic monitoring. These modalities often limited in terms accessibility as well their ability to map brain perfusion with sufficient spatial temporal resolution, particularly context preclinical studies, thus calling development new techniques featuring rapid speed, cost-effectiveness, ease use. Herein, we report on...

Immune therapy has become the fourth method to treat tumors after traditional methods including radiotherapy, chemotherapy and surgery. therapies use humans’ innate immune system fight against tumors. Nowadays there are many used in clinic, checkpoint inhibitors, cancer vaccines, cytokine therapies, so on. One of most mature widely is inhibitors (ICIs). Because tumor surface have some proteins that can bind receptors on cells, once they bound function will be inhibited live human bodies not...

Abstract C 22 H 19 NO 7 , monoclinic, P 2 1 / c (no. 14), a = 9.292(5) Å, b 13.230(3) 17.115(4) β 106.04(5)°, V 2022.1(14) Å 3 Z 4, R gt ( F ) 0.0441, wR ref 0.1156, T 293(2) K.