A5031041750- Spaceflight effects on biology
- Mesenchymal stem cell research
- Human Health and Disease
- Metabolomics and Mass Spectrometry Studies
- Erythrocyte Function and Pathophysiology
- Muscle Physiology and Disorders
- Cardiovascular Disease and Adiposity
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer, Hypoxia, and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Advanced Proteomics Techniques and Applications
- Thermoregulation and physiological responses
- Healthcare Systems and Public Health
- S100 Proteins and Annexins
- Hematopoietic Stem Cell Transplantation
- Space Exploration and Technology
- Hippo pathway signaling and YAP/TAZ
- Effects of Radiation Exposure
- Axon Guidance and Neuronal Signaling
- Bone fractures and treatments
- Body Composition Measurement Techniques
- Cardiovascular Health and Risk Factors
- Electromagnetic Fields and Biological Effects
- Prosthetics and Rehabilitation Robotics
- Microtubule and mitosis dynamics
Institute of Biomedical Problems
2017-2024
Introduction: Prolonged space flights negatively affect skeleton. Stromal cells of mesenchymal origin play a crucial role in maintaining homeostasis and regulating the physiological remodeling various tissues, this has particular significance for bone. Methods: Hindlimb unloading (HU) rats as ground-based model simulation microgravity was implemented. The functional activity skeletal stem progenitor (SSPCs) from rat femoral bones assessed vitro after 2 weeks HU subsequent recovery load...
Space missions provide the opportunity to investigate influence of gravity on dynamic remodelling processes in bone. Mice were examined following space flight and subsequent recovery determine effects bone compartment-specific microstructure composition. The resulting loss microgravity recovered only trabecular bone, while cortical tissue mineral density was restored after one week Earth. Detection TRAP-positive surface cells compartment indicated increased resorption flight. In a persistent...
Elucidation of the spaceflight (SF) effects on adult stem and progenitor cells is an important goal in space biology medicine. A unique opportunity for this was provided by project "BION-M1". The purpose study to evaluate 30-day SF biosatellite, 7-day recovery (SFR), subsequent ground control (GC) experiment mononuclear (MNCs) from C57/BI/6N murine tibia bone marrow. Also, hematopoietic stromal precursor functions were characterized ex vivo. There no significant difference total MNC number...
Muscle and skeleton structures are considered most susceptible to negative factors of spaceflights, namely microgravity. Three-dimensional clinorotation is a ground-based simulation It provides an opportunity elucidate the effects microgravity at cellular level. The extracellular matrix (ECM) content, transcriptional profiles genes encoding ECM remodelling molecules, secretory were investigated in heterotypic primary culture bone marrow cells after 14 days 3D clinorotation. Simulated...
Functional activity of the bone marrow cells is dependent on external signals received from a local microenvironment. In addition to soluble growth factors and hormones, contacts with neighbor extracellular matrix are also most important homeostasis regulators in niche. Purpose work explore effects simulated microgravity transcription genes products which define cell-to-cell cell-to-matrix interactions micro-explants. Femoral male mice BalbC 19-20 wks age were used investigation. After...
The development of osteopenia is one the most noticeable manifestations adverse effects space factors on crew members. Hippo signaling pathway has been shown to play a central role in regulating functional activity cells through their response mechanical stimuli. In present study, components and protective properties osteodifferentiation inducers were investigated under simulated microgravity (smg) using heterotypic bone marrow cell culture model, which allows for maintenance close...
Blood samples from six 25–40 y.o. male subjects in a 5-d dry immersion (DI) experiment were analyzed for cholesterol, cholesterol of high- (HDLP), low- (LDLP) and very low density lipoproteins, triglycerides, apolipoproteins А1 (аpoА1) В (аpоВ), non-esterified (free) fatty acids (NEFA), β-hydroxibutyrate phospholipids. Atherogenic indices calculated, as well the ratios аpоВ/аpоА1 cholesterol/phospholipids. No significant changes or lipoprotein fractions determined. Increases triglyceride...
Bone and muscle tissues are mostly susceptible to different kinds of hypodynamia, including real simulated microgravity (sμg). To evaluate the effect sμg on bone marrow (BM), male C57Bl/6N mice were divided into three groups: vivarium control (VC), 30-day hindlimb suspension (HS), subsequent 12-h short-term support reloading (RL). The effects BM total mononucleated cells (MNCs) as well stromal hematopoietic progenitors from murine tibia studied. number MNCs, immunophenotype, proliferation,...
Rodent hindlimb unloading (HU) model was developed to elucidate responses/mechanisms of adverse consequences space weightlessness. Multipotent mesenchymal stromal cells (MMSCs) were isolated from rat femur and tibia bone marrows examined ex vivo after 2 weeks HU subsequent restoration load (HU + RL). In both bones, decrease fibroblast colony forming units (CFU-f) with RL detected. CFU-f MMSCs, levels spontaneous/induced osteocommitment similar. MMSCs initially had greater spontaneous...
8 male volunteers aged 26 to 44 years were examined, who in the “Arfa” hypomagnetic environment simulation facility for 4 hours at a normal magnetic field level (background session), and twice within 24 hours: one session during simulation. with an attenuation coefficient of 500, − conditions terrestrial (placebo). Venous blood was collected end each session. The values 48 biochemical parameters reflecting state organs tissues, as well main links metabolism, determined plasma serum....