A5050134567- Bone health and osteoporosis research
- Orthopaedic implants and arthroplasty
- Bone Metabolism and Diseases
- Connective tissue disorders research
- Bone health and treatments
- Bone and Joint Diseases
- Orthopedic Infections and Treatments
- Dermatological and Skeletal Disorders
- Erythrocyte Function and Pathophysiology
- Bone fractures and treatments
- Blood properties and coagulation
- Alkaline Phosphatase Research Studies
- Total Knee Arthroplasty Outcomes
- Heterotopic Ossification and Related Conditions
- Spinal Fractures and Fixation Techniques
- Spine and Intervertebral Disc Pathology
- Genetic factors in colorectal cancer
- Parathyroid Disorders and Treatments
- Infectious Diseases and Tuberculosis
- Hip disorders and treatments
- Cancer-related gene regulation
- Pelvic and Acetabular Injuries
- Osteoarthritis Treatment and Mechanisms
- Selenium in Biological Systems
- Proteoglycans and glycosaminoglycans research
University Medical Center Hamburg-Eppendorf
2019-2025
Universität Hamburg
2019-2025
Abstract Piezo proteins are mechanically activated ion channels, which required for mechanosensing functions in a variety of cell types. While we and others have previously demonstrated that the expression Piezo1 osteoblast lineage cells is essential bone-anabolic processes, there was only suggestive evidence indicating role and/or Piezo2 cartilage. Here addressed question if how chondrocyte mechanosensitive or controls physiological endochondral ossification pathological osteoarthritis (OA)...
The skeleton is a dynamic tissue continuously adapting to mechanical stimuli. Although matrix-embedded osteocytes are considered as the key mechanoresponsive bone cells, all other skeletal cell types principally exposed macroenvironmental and microenvironmental influences that could potentially affect their activities. It was recently reported Piezo1, one of two mechanically activated ion channels Piezo family, functions mechanosensor in osteoblasts osteocytes. Here we show Piezo1...
Immobilization as a result of long-term bed rest can lead to gradual bone loss. Because their distribution throughout the matrix and remarkable interconnectivity, osteocytes represent major mechanosensors in translate mechanical into biochemical signals controlling remodeling. To test whether immobilization affects characteristics osteocyte network human cortical bone, femoral diaphyseal specimens were analyzed immobilized female individuals compared with age-matched postmenopausal primary...
ABSTRACT Biallelic ENPP1 deficiency in humans induces generalized arterial calcification of infancy (GACI) and/or autosomal recessive hypophosphatemic rickets type 2 (ARHR2). The latter is characterized by markedly increased circulating FGF23 levels and renal phosphate wasting, but aberrant skeletal manifestations associated with heterozygous are unknown. Here, we report three adult men early onset osteoporosis who presented fractures the thoracic spine left radius, mildly elevated FGF23,...
Abstract Background In atlantoaxial instabilities, posterior C1/C2 fusion using lateral mass screws (LMS) or pedicle (PS) in a mono- bicortical position the atlas is typical treatment. The bone microstructure and positioning of screw trajectories appear to be significant relevance for stability. Purpose aim this study was comparative analysis mechanical durability fixation concerning microstructural characteristics LMS PS position. Methods Human C1 from geriatric body donors ( n = 28; 50%...
The recent identification of homozygous WNT1 mutations in individuals with osteogenesis imperfecta type XV (OI-XV) has suggested that is a key ligand promoting the differentiation and function bone-forming osteoblasts. Although such an influence was supported by subsequent studies, mouse model OI-XV remained to be established. Therefore, we introduced previously identified disease-causing mutation (G177C) into murine Wnt1 gene. Homozygous Wnt1G177C/G177C mice were viable did not display...
ABSTRACT Inactivating mutations in human ecto-nucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) may result early-onset osteoporosis (EOOP) haploinsufficiency and autosomal recessive hypophosphatemic rickets (ARHR2) homozygous deficiency. ARHR2 patients are frequently treated with phosphate supplementation to ameliorate the rachitic phenotype, but elevating plasma phosphorus concentrations increase risk of ectopic calcification without increasing bone mass. To assess risks efficacy...
ObjectiveThe subchondral bone is an emerging regulator of osteoarthritis (OA). However, knowledge how specific alterations relate to cartilage degeneration remains incomplete.MethodFemoral heads were obtained from 44 patients with primary OA during total hip arthroplasty and 30 non-OA controls autopsy. A multiscale assessment the central region comprising histomorphometry, quantitative backscattered electron imaging, nanoindentation, osteocyte lacunocanalicular network characterization was...
The high occurrence of distal fibula fractures among older women suggests a potential link to impaired bone health. Here we used multiscale imaging approach investigate the microarchitecture, mineralization, and biomechanics human in relation age sex. Micro-computed tomography was performed analyze local volumetric mineral density various microarchitectural parameters trabecular cortical compartment. Bone distribution osteocyte lacunar were quantified using quantitative backscattered...
Surgical intervention with intercorporal stabilisation in spinal infections is increasingly needed. Our aim was to compare titanium and polyetheretherketon (PEEK) cages according their adhesion characteristics of different bacteria species vitro.Plates made from PEEK, polished (Ti), two-surface-titanium (TiMe) (n = 2-3) original PEEK porous trabecular structured (TiLi) interbody 4) were inoculated bacterial solutions, S.aureus (MSSA, MRSA), S.epidermidis E.coli. Growth analysed. Biofilms...
Abstract Skeletal growth, modeling, and remodeling are regulated by various molecules, one of them being the recently identified osteoanabolic factor WNT1. We have previously reported that WNT1 transcriptionally activates expression Omd, encoding Osteomodulin (OMD), in a murine mesenchymal cell line, which potentially explained skeletal fragility mice with mutational inactivation, since OMD has been shown to regulate type I collagen fibril formation vitro. In this study we confirmed strong...
Biallelic ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) deficiency induces vascular/soft tissue calcifications in generalized arterial calcification of infancy (GACI), and low bone mass with phosphate-wasting rickets GACI survivors (autosomal hypophosphatemic type-2). ENPP1 haploinsufficiency early-onset osteoporosis mild phosphate wasting adults. Both conditions demonstrate the unusual combination reduced accrual skeletal mineral, yet excess progressive heterotopic...
Space missions provide the opportunity to investigate influence of gravity on dynamic remodelling processes in bone. Mice were examined following space flight and subsequent recovery determine effects bone compartment-specific microstructure composition. The resulting loss microgravity recovered only trabecular bone, while cortical tissue mineral density was restored after one week Earth. Detection TRAP-positive surface cells compartment indicated increased resorption flight. In a persistent...
Background: Impacted bone-grafting with morselized allograft chips is commonly used to reconstruct acetabular bone defects in revision total hip arthroplasty (THA). While the overall clinical outcome of this procedure described be excellent, microstructural basis and histological determinants incorporation remained further elucidated. Methods: The acetabula 23 individuals documented previous use during THA were explanted post mortem. time that allografts situ averaged 10.3 ± 4.5 years...
Abstract Background Heterotopic ossification (HO) is a common complication after THA. Although current research primarily focuses on treatment and prevention, little known about the local bone metabolism of HO clinical contributing factors. Questions/purposes We aimed to assess remodeling processes in using histomorphometry, focusing effects inflammation prior NSAID treatment. Specifically, we asked: (1) Are specimens taken from patients with periprosthetic joint infection (PJI) more likely...
Abstract Hypophosphatasia (HPP) is an inherited, systemic disorder, caused by loss-of-function variants of the ALPL gene encoding enzyme tissue non-specific alkaline phosphatase (TNSALP). HPP characterized low serum TNSALP concentrations associated with defective bone mineralization and increased fracture risk. Dental manifestations have been reported as exclusive feature (odontohypophosphatasia) in combination skeletal complications. Enzyme replacement therapy (asfotase alfa) has shown to...
Abstract Coffin–Lowry–Syndrome (CLS) is a X-linked mental retardation characterized by skeletal dysplasia and premature tooth loss. We others have previously demonstrated that the ribosomal S6 kinase RSK2, mutated in CLS, essential for bone cementum formation; however, it remains to be established whether RSK2 plays also role mechanically induced remodeling during orthodontic movement (OTM). We, therefore, performed OTM wild-type (WT) mice Rsk2-deficient using Nitinol tension springs were...
Background/Aim: The aim of this study was to present the long-term course a patient with nevus sebaceous syndrome (NSS). Recent genetic studies place in emerging group so-called RASopathies. focus report is on surgical treatment and morphological findings face oral cavity. Case Report: A female treated for congenital alterations facial skin mucosa. lesions were removed repeatedly. Eruption teeth lesion sites made easier by measures taken. However, after repeated ablation affected gingiva,...
Mutations in the gene ANO5, encoding for transmembrane protein Anoctamin 5 (Ano5), have been identified to cause gnathodiaphyseal dysplasia (GDD) humans, a skeletal disorder characterized by sclerosis of tubular bones, increased fracture risk and fibro-osseous lesions jawbones. To better understand pathomechanism GDD we generated via Crispr/CAS9 editing mouse model harboring murine equivalent (Ano5 p.T491F) GDD-causing ANO5 mutation previously reported patient. Skeletal phenotyping contact...