A5014965463- Diabetes and associated disorders
- Diabetes Management and Research
- Pancreatic function and diabetes
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Marine Bivalve and Aquaculture Studies
- Ubiquitin and proteasome pathways
- Aquaculture Nutrition and Growth
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biochemical and Structural Characterization
- Aortic Disease and Treatment Approaches
- Advanced Fluorescence Microscopy Techniques
- Aquaculture disease management and microbiota
- Additive Manufacturing and 3D Printing Technologies
- Metabolism, Diabetes, and Cancer
- Cardiovascular and Diving-Related Complications
- Glycosylation and Glycoproteins Research
- Heavy Metal Exposure and Toxicity
- Erythrocyte Function and Pathophysiology
- Advanced Glycation End Products research
- Peptidase Inhibition and Analysis
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Orthopedic Infections and Treatments
NOAA National Marine Fisheries Service Alaska Fisheries Science Center
2022-2024
NOAA National Marine Fisheries Service
2022-2024
Michigan State University
2017-2022
Quantitative BioSciences
2022
In-Q-Tel
2020
Microfluidic devices have historically been prepared using fabrication techniques that often include photolithography and/or etching. Recently, additive manufacturing technologies, commonly known as 3D-printing, emerged tools for microfluidic devices. Unfortunately, PolyJet which utilizes a photocurable resin can be accurately printed, requires the use of support material any designed void space internal to model. Removing from printed channels is difficult in small with single dimensions...
Wide accessibility and a broad range of applications have made 3D-printers commonplace tool in the science community. From tier-one research institutions to community public libraries high schools, are being used enrich STEM education through variety learning techniques experiences. Reports 3D-printed models for improved visualization chemical phenomena, as well educational use laboratory devices, rapidly increasing. The objective this review is provide resource educators interested...
Equilibrium dialysis is a simple and effective technique used for investigating the binding of small molecules ions to proteins. A three-dimensional (3D) printer was create device capable measuring constants between protein ion based on equilibrium dialysis. Specifically, technology described here enables user customize an fit their own experiments by choosing membranes various material molecular-weight cutoff values. The has dimensions similar that standard 96-well plate, thus being...
Microfluidic devices control fluids on the micrometer-scale and are commonly used for lab-on-chip applications, such as sensors, micropumps biological analyzers. Commonly reported fabrication methods achieving flexible microfluidic structures labor-intensive, require many cumbersome steps, have limited options materials. This paper presents a rapid-manufacturing technique using PolyJet 3D-printer creating soft substrates embedded with liquid metals to fabricate stretchable conductors pressure...
Plasma protein binding measurements are an important aspect of pharmacology and drug development. Therefore, performing these can provide a valuable highly practical learning experience for students across many scientific disciplines. Here, we describe the design characterization 3D-printed device capable equilibrium dialysis in order to measure affinity between fluorescent ligand common plasma protein. The is designed laboratory automation, having dimensions identical those standard 96-well...
Protein conformational switches or allosteric proteins play a key role in the regulation of many essential biological pathways. Nonetheless, implementation protein design applications has proven challenging, with only few known examples that are not derivatives naturally occurring systems. We have discovered domain-swapped (DS) dimer hCRBPII undergoes large and robust change upon retinal binding, making it potentially powerful template for switches. Atomic resolution structures apo-...
Here, we analyze the performance of a microplate assay utilizing sulfo-phospho-vanillin (SPV) reaction to quantify total lipid content various large crustacean zooplankton in rapid and high throughput manner.
Plasma proteins are covalently modified in vivo by the high-glucose conditions bloodstreams of people with diabetes, resulting changes to both structure and function. Human Serum Albumin (HSA) functions as a carrier-protein bloodstream, binding various ligands tightly regulating their bioavailability. HSA is known react glucose via Maillard reaction, causing adverse effects on its ability bind deliver certain ligands, such metals. Here, between glycated zinc (Zn2+) was determined using novel...
Abstract People with type 1 diabetes (T1D) require exogenous administration of insulin, which stimulates the translocation GLUT4 glucose transporter to cell membranes. However, most bloodstream cells contain GLUT1 and are not directly affected by insulin. Here, we report that C-peptide, 31-amino acid peptide secreted in equal amounts insulin vivo, is part a 3-component complex affects red blood (RBC) Multiple techniques were used demonstrate saturable specific C-peptide binding RBCs when...
Domain-swapping is a mechanism for evolving new protein structure from extant scaffolds, and has been an efficient protein-engineering strategy tailoring functional diversity. However, domain swapping can only be exploited if it controlled, especially in cases where various folds coexist. Herein, we describe the of domain-swapped trimer iLBP family member hCRBPII, suggest trimerization. It further shown that trimerization favored by strategic installation disulfide bond, thus demonstrating...
Abstract Total lipid content is a valuable indicator of fish health, prey quality, survival potential, stock and ecosystem status. Here, we demonstrate an accurate method for measuring total lipids in tissues using the spectrophotometric sulfo‐phospho‐vanillin (SPV) assay, adapted to 96‐well plate format. Samples dried homogenate were cross‐analyzed via SPV assay standard gravimetric analysis. Initial measurements whole homogenates analyzed include Pacific herring ( Clupea pallasii ), cod...
Blooms of Alexandrium catenella threaten to disrupt subsistence, recreational, and commercial shellfish harvest in Alaska, as the paralytic toxins (PSTs) produced pose a serious public health risk can lead costly shutdowns for farmers. Current methods PST detection region range from monitoring programs utilizing net tows detect A. direct tissue testing via mouse bioassay (MBA) aquaculture harvest, well various optional subsistence recreational harvesters. The efficacy feasibility these vary,...
Diabetes affects 30 million individuals in the U.S. today. Approximately 10% of these cases are type 1 diabetes (T1D). T1D is an auto‐immune disease that destroys insulin‐producing cells pancreas (β‐cells), resulting increased levels blood glucose (hyperglycemia). Consequently, people with suffer from many vascular health complications, such as nephropathy, neuropathy, retinopathy, and cardiovascular disease. In addition, immune deficient. It hypothesized hyperglycemia effects expression...
C‐peptide, a hormone that is found in the pancreatic β‐cell, co‐secreted equimolar amounts to insulin, and has been shown alleviate microvascular complications diabetes. Prior research indicated C‐peptide requires complex of human serum albumin (HSA) zinc (Zn 2+ ) be transported bloodstream elicit its effects. HSA prominent plasma protein involved transporting many different molecules throughout body. Due physiological conditions attributed type 1 diabetes (T1D) there both an increase...
The purpose of this study was to investigate the antimicrobial efficacy a novel activated zinc solution against meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa after one hour, evaluate any untoward effect on local wound tissue at 24 hours exposure in pig model.A pathogen-free, commercially raised, Yorkshire-cross female acquired 12 days prior procedure. Within week procedure, small loopful test bacteria, (pig-isolate) MRSA (ATCC-6538), were streaked cultured...