A5017739660- Microbial Metabolic Engineering and Bioproduction
- Biochemical and biochemical processes
- Fermentation and Sensory Analysis
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Plant Disease Resistance and Genetics
- Fungal Plant Pathogen Control
- Cell Image Analysis Techniques
- Phytochemicals and Antioxidant Activities
- GABA and Rice Research
- Entomopathogenic Microorganisms in Pest Control
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Protist diversity and phylogeny
- Mycorrhizal Fungi and Plant Interactions
- Bacterial Identification and Susceptibility Testing
- Insect symbiosis and bacterial influences
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
Westerdijk Fungal Biodiversity Institute
2017-2025
University Medical Center Utrecht
2022
Hubrecht Institute for Developmental Biology and Stem Cell Research
2022
Utrecht University
2017-2021
Wageningen University & Research
2015
Section Flavi encompasses both harmful and beneficial Aspergillus species, such as oryzae, used in food fermentation enzyme production, flavus, spoiler mycotoxin producer. Here, we sequence 19 genomes spanning section compare 31 fungal including 23 species. We reassess their phylogenetic relationships show that the closest relative of A. oryzae is not but minisclerotigenes or aflatoxiformans identify high genome diversity, especially sub-telomeric regions. predict abundant CAZymes (598 per...
Aspergillus niger produces an arsenal of extracellular enzymes that allow synergistic degradation plant biomass found in its environment. Pectin is a heteropolymer abundantly present the primary cell wall plants. The complex structure pectin requires multiple to act together. Production pectinolytic A. highly regulated, which allows flexible and efficient capture nutrients. So far, three transcriptional activators have been linked regulation niger. L-rhamnose-responsive regulator RhaR...
Abstract Background The aromatic compounds vanillin and vanillic acid are important fragrances used in the food, beverage, cosmetic pharmaceutical industries. Currently, most products chemically synthesized, while only a small percentage is extracted from natural sources. metabolism of has been studied for decades microorganisms many studies have conducted that showed both can be produced ferulic using bacteria. In contrast, degradation by fungi poorly no genes involved this metabolic...
The aromatic compound benzoic acid and its derivatives are frequently used preservatives in food beverages due to their strong antimicrobial properties. Benzoic naturally occurs plants serves as a building block for primary secondary metabolites. Several Aspergilli able degrade p-hydroxybenzoic by p-hydroxylation followed m-hydroxylation protocatechuic acid. ring of is then cleaved further converted through the oxoadipate pathway where it carbon source. well studied filamentous fungus...
Carbon catabolite repression enables fungi to utilize the most favourable carbon source in environment, and is mediated by a key regulator, CreA, fungi. CreA-mediated regulation has mainly been studied at high monosaccharide concentrations, an uncommon situation natural biotopes. In nature, many rely on plant biomass as their major producing enzymes degrade cell wall polysaccharides into metabolizable sugars. To determine role of CreA when grow more conditions particular with respect...
D-galacturonic acid (GalA) is the most abundant monosaccharide component of pectin. Previous transcriptome analysis in plant pathogenic fungus Botrytis cinerea identified eight GalA-inducible genes involved pectin decomposition, GalA transport and utilization. Co-expression these indicates that a specific regulatory mechanism occurs B. cinerea. In this study, promoter regions were analysed conserved sequence motifs identified. The Bclga1 promoter, containing all motifs, was functionally...
Salicylic acid plays an important role in the plant immune response, and its degradation is therefore for plant-pathogenic fungi. However, many nonpathogenic microorganisms can also degrade salicylic acid. In filamentous fungus Aspergillus niger, two metabolic pathways have been suggested. The first pathway converts to catechol by a salicylate hydroxylase (ShyA). second pathway, 3-hydroxylated 2,3-dihydroxybenzoic acid, followed decarboxylation 2,3-dihydroxybenzoate decarboxylase (DhbA). A....
Abstract Aromatic compounds are important molecules which widely applied in many industries and mainly produced from nonrenewable sources. Renewable sources such as plant biomass interesting alternatives for the production of aromatic compounds. Ferulic acid p- coumaric acid, a precursor vanillin p -vinyl phenol, respectively, can be released by fungus Aspergillus niger . The degradation hydroxycinnamic acids caffeic ferulic -coumaric has been observed fungi. In A. , multiple metabolic...
Protocatechuic acid (3,4-dihydroxybenzoic acid) is a chemical building block for polymers and plastics. In addition, protocatechuic has many properties of great pharmaceutical interest. Much research been performed in creating bacterial production strains, but no acid-producing fungal cell factories have described. The filamentous fungus Aspergillus niger can produce as an intermediate the benzoic metabolic pathway. Recently, p-hydroxybenzoate-m-hydroxylase (phhA) protocatechuate...
Cinnamic acid is an aromatic compound commonly found in plants and functions as a central intermediate lignin synthesis. Filamentous fungi are able to degrade cinnamic through multiple metabolic pathways. One of the best studied pathways non-oxidative decarboxylation styrene. In Aspergillus niger, enzymes decarboxylase (CdcA, formally ferulic decarboxylase) flavin prenyltransferase (PadA) catalyze together sorbic acid. The corresponding genes, cdcA padA, clustered genome with putative...
Antimicrobial resistance is a major threat to human health. Basic knowledge of antimicrobial mechanism action (MoA) imperative for patient care and identification novel antimicrobials. However, the process MoA relatively laborious. Here, we developed simple, quantitative time-lapse fluorescence imaging method, Dynamic Bacterial Morphology Imaging (DBMI), facilitate this process. It uses membrane dye nucleoid track morphological changes single Bacillus subtilis cells in response...
Trichoderma reesei is one of the major producers enzymes for conversion plant biomass to sustainable fuels and chemicals. Crude can induce production CAZymes in T. reesei, but there limited understanding how transcriptional response crude regulated. In addition, it unknown whether induction on untreated recalcitrant (with a large diversity inducers) be sustained longer. We investigated transcriptomic two industrial feedstocks, corn stover (CS) soybean hulls (SBH), over time (4 h, 24 h 48 h),...
To create an Aspergillus niger mutant with increased tolerance against ferulic acid using evolutionary adaptation.Evolutionary adaptation of A. N402 was performed by consecutive growth on increasing concentrations in the presence 25 mmol l-1 d-fructose, starting from 0·5 and ending 5 acid. The obtained after six months, named Fa6, showed compared to parent. In addition, Fa6 has consumption a higher conversion rate, suggesting that mutation affects aromatic metabolism this species....