A5038620462- DNA and Nucleic Acid Chemistry
- Advanced Fluorescence Microscopy Techniques
- HER2/EGFR in Cancer Research
- Photochromic and Fluorescence Chemistry
- Probiotics and Fermented Foods
- Angiogenesis and VEGF in Cancer
- HIV/AIDS drug development and treatment
- Chemical Reactions and Isotopes
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Genomics, phytochemicals, and oxidative stress
- Photodynamic Therapy Research Studies
- Synthesis and Reactivity of Heterocycles
- Cancer Treatment and Pharmacology
- Bacteriophages and microbial interactions
- Biopolymer Synthesis and Applications
- Photosynthetic Processes and Mechanisms
- Chronic Lymphocytic Leukemia Research
- Medical and Biological Ozone Research
- Monoclonal and Polyclonal Antibodies Research
- Protein Tyrosine Phosphatases
- Biochemical and Molecular Research
- Chronic Myeloid Leukemia Treatments
- Metabolism and Genetic Disorders
- interferon and immune responses
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2020-2023
General University Hospital in Prague
2022
Charles University
2022
University of Cambridge
2022
Comenius University Bratislava
1995-2018
Institute of Cell Biology
2002
The 3'-5', 3'-5' cyclic dinucleotides (3'3'CDNs) are bacterial second messengers that can also bind to the stimulator of interferon genes (STING) adaptor protein in vertebrates and activate host innate immunity. Here, we profiled substrate specificity four dinucleotide synthases from Vibrio cholerae (DncV), Bacillus thuringiensis (btDisA), Escherichia coli (dgcZ), Thermotoga maritima (tDGC) using a library 33 nucleoside-5'-triphosphate analogues then employed these enzymes synthesize 24...
3-Acylmethylidene-3,4-dihydroquinoxalin-2(1 H )-ones are compounds which possess a wide range of physical and pharmaceutical applications. These can be easily prepared by cyclocondensation o -phenylenediamines aroylpyruvates. Unsymmetrically substituted obtained form regioisomeric mixtures 3,4-dihydroquinoxalin-2(1 )-ones. It is often quite difficult to get pure regioisomer determine its structure without controlling the reaction selectivity exploitation complex NMR techniques (HSQC, NOESY,...
Abstract The formation of a G‐quadruplex motif in the promoter region c‐MYC protooncogene prevents its expression. Accordingly, stabilization by suitable ligand may be viable approach for anticancer therapy. In our study, we used 4‐(4‐methylpiperazin‐1‐yl)aniline molecule, previously identified as fragment library screen hit, template SAR‐guided design new small clickable fragments and subjected them to click reactions, including kinetic target‐guided synthesis presence forming...
Abstract The secondary structure of nucleic acids containing quartets guanines, termed G-quadruplexes, is known to regulate the transcription many genes. Several G-quadruplexes can be formed in HIV-1 long terminal repeat promoter region and their stabilization results inhibition replication. Here, we identified helquat-based compounds as a new class anti-HIV-1 inhibitors that inhibit replication at stage reverse provirus expression. Using Taq polymerase stop FRET melting assays, have...
Abstract DNA-binding fluorescent probes are useful tools in cell biology for various applications enabling visualisation and quantification of DNA. Herein, we report on HeliDye1 , a novel cationic helicene-like DNA probe, its versatile usability. exhibits fluorescence exclusively the presence duplexes containing AT base pairs (contrary to any recent probe) it remains non-fluorescent single-stranded RNA. Favourable chemical properties allowed use karyotyping super-resolution microscopy. We...
Abstract DNA-binding fluorescent probes are useful tools in cell biology for various applications enabling visualisation and quantification of DNA. Herein, we report on HeliDye1 , a novel cationic helicene-like DNA probe, its versatile usability. exhibits fluorescence exclusively the presence duplexes containing AT base pairs (contrary to any recent probe) it remains non-fluorescent single-stranded RNA. Favourable chemical properties allowed use super-resolution microscopy using flow...