A5022130253- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Pituitary Gland Disorders and Treatments
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Crystallography and molecular interactions
- Adrenal and Paraganglionic Tumors
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- Collembola Taxonomy and Ecology Studies
- Viral Infections and Immunology Research
- Advanced Chemical Physics Studies
- Neuroendocrine Tumor Research Advances
- Pharmacological Effects of Natural Compounds
- Fossil Insects in Amber
- Fish Biology and Ecology Studies
- Neuropeptides and Animal Physiology
- Growth Hormone and Insulin-like Growth Factors
- Nicotinic Acetylcholine Receptors Study
- Cholinesterase and Neurodegenerative Diseases
- Insect and Arachnid Ecology and Behavior
- Study of Mite Species
- Evolution and Genetic Dynamics
- Folate and B Vitamins Research
Sichuan University
2013-2025
West China Hospital of Sichuan University
2013-2025
Northwest University
2025
Gannan Medical University
2025
Fudan University
2023-2025
Chinese University of Hong Kong
2025
University of Hong Kong
2025
Shanghai Jiao Tong University
2020-2024
Ruijin Hospital
2020-2024
Biomedical Research Institute
2024
The development of a theoretical databank transferable pseudoatoms for fast prediction the electron densities and related electronic properties proteins is described. Chemically unique identified on basis common connectivity bonding are extracted from ab initio molecular large number small molecules using least-squares projection technique in Fourier transform space. performance evaluated by comparison electrostatic amino acids GLN, SER, LEU their dimers with those obtained calculations same...
Abstract The Drosophila Eyes Absent Homologue 1 (EYA1) is a component of the retinal determination gene network and serves as an H2AX phosphatase. cyclin D1 encodes regulatory subunits holoenzyme that phosphorylates inactivates pRb protein. Herein, comparison with normal breast showed EYA1 overexpressed in luminal B cancer subtype. enhanced tumor growth mice vivo, requiring phosphatase domain. cellular proliferation, inhibited apoptosis, induced contact-independent abundance. induction...
Background/Aims: Enriched environment (EE) has been reported to exert neuroprotective effect in animal models of ischemic stroke. However, the underlying mechanism remains unclear. The purpose this study was investigate EE treatment on neuronal apoptosis periinfarct cortex after cerebral ischemia/reperfusion (I/R) injury. Methods: I/R injury established by middle artery occlusion (MCAO). A set behavioral tests including modified neurological severity score (mNSS), limb-placing test and...
Abstract G protein-coupled receptors (GPCRs) can integrate extracellular signals via allosteric interactions within dimers and higher-order oligomers. However, the structural bases of these remain unclear. Here, we use GABA B receptor heterodimer as a model it forms large complexes in brain. It is subjected to genetic mutations mainly affecting transmembrane 6 (TM6) involved human diseases. By cross-linking, identify interfaces B1 -GABA B2 , well interactions. Our data are consistent with an...
Abstract An ongoing question regarding the energetics of protein‐ligand binding has been; what is strain energy that a ligand pays (if any) when to its protein target? The traditional method estimate uses force fields calculate difference between bound conformation and nearest local minimum/global minimum on gas‐phase or aqueous phase potential surface. This makes implicit assumption underlying field as well reference crystal structure accurate. Herein, we use ibuprofen test case compare...
G protein-coupled receptors (GPCRs) are among the most promising drug targets. They often form homo- and heterodimers with allosteric cross-talk between receptor entities, which contributes to fine-tuning of transmembrane signaling. Specifically controlling activity GPCR dimers ligands is a good approach clarify their physiological roles validate them as Here, we examined mode action positive modulators (PAMs) that bind at interface domains heterodimeric GABA B receptor. Our site-directed...
The KREMEN1 (KRM1) protein is a cellular receptor for multiple enteroviruses that cause hand, foot, and mouth disease (HFMD), including coxsackievirus CVA2, CVA3, CVA4, CVA5, CVA6, CVA10, CVA12. molecular basis the broad recognition of these viruses by KRM1 remains unclear. Here, we report indispensable role completely conserved VP2 capsid residue K140 (designated K2140) in mediating infection CVA10 other KRM1-dependent enteroviruses. Residue K2140 not only facilitates recognition, cell...
Lung cancer is a malignant tumor with high morbidity and mortality rate worldwide, causing an increasing disease burden. Of these, the most common type non-small cell lung (NSCLC), which accounts for 80-85% of all cases. Genetic research crucial continuously discovering susceptibility genes related to in-depth study. The role genetic predisposition in development NSCLC, particularly within circadian rhythm pathways known govern various physiological processes, increasingly acknowledged. Yet,...
Chronic obstructive pulmonary disease (COPD) imposes a significant and growing burden on China the world. Early detection diagnosis may be an effective way to alleviate this severe pressure public health. The Enjoying Breathing Program (the Program), nationwide one-time two-step COPD screening management program with long-term follow-up initiated by World Health Organization Collaborating Centre (WHO CC), has demonstrated its clinical benefit. However, cost-effectiveness of remains unknown....
Abstract Background Pituitary neuroendocrine tumors (PitNETs) are common intracranial that classified into seven histological subtypes, including lactotroph, somatotroph, corticotroph, thyrotroph, gonadotroph, null cell, and plurihormonal PitNETs. However, the molecular characteristics of these types PitNETs not completely clear. Methods A total 180 consecutive cases were collected to perform RNA sequencing. All subtypes distinguished by unsupervised clustering analysis. We investigated...
The rapid proliferation and colonization of probiotics in the intestines are essential for human health. Quorum sensing (QS) is a communication mechanism among bacteria, which can regulate various bacterial crowd behavior. This study aimed to enhance viability Lactobacillus reuteri 1-12 by regulating QS. Herein, we built database containing 72 natural products (previously reported) that improve intestinal flora. Virtual screening (VS) was subsequently conducted screen four potential active...
Ductal carcinoma in situ with microinvasion (DCISM) was defined as one or more foci of invasion beyond the basement membrane within 1 mm. The size primary lesion is associated axillary status and prognosis patients invasive breast cancer; thus, it interest to determine whether multiple are a higher risk positive worse long-term outcomes DCISM.This study identified 359 DCISM who had undergone evaluation at our institute from January 2006 December 2015. Patients were categorized focus (≥2...
Determining the structure of a small molecule bound to biological receptor (e.g., protein implicated in disease state) is necessary step structure-based drug design. The preferred conformation can change when protein, and detailed knowledge conformation(s) ligand help optimizing affinity for its receptor. However, quality protein/ligand complex determined using X-ray crystallography dependent on size crystal quality, realized resolution. energy restraints used traditional refinement...