A5101426742- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Gene Regulatory Network Analysis
- Stress Responses and Cortisol
- Molecular Sensors and Ion Detection
- Wnt/β-catenin signaling in development and cancer
- Sleep and Wakefulness Research
- Axon Guidance and Neuronal Signaling
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Photoreceptor and optogenetics research
Max Planck Florida Institute for Neuroscience
2018-2021
Osaka University
2014-2016
National Institute of Biomedical Innovation, Health and Nutrition
2016
Lymphocyte recirculation through secondary lymphoid organs is essential for immunosurveillance and lymphocyte effector functions. Here, we show that signals β2-adrenergic receptors (β2ARs) expressed on lymphocytes are involved in the control of dynamics by altering responsiveness chemoattractant receptors. Agonist stimulation β2ARs inhibited egress from lymph nodes (LNs) rapidly produced lymphopenia mice. Physiological inputs adrenergic nerves contributed to retention within LNs homeostasis...
Various aspects of the immune system display circadian rhythms. Although lymphocyte trafficking has been suggested to show diurnal variations, mechanisms and influences on responses are unclear. Here, we in mice that inputs from adrenergic nerves contribute variation recirculation through lymph nodes (LNs), which is reflected magnitude adaptive response. Neural β2-adrenergic receptors (β2ARs) expressed lymphocytes reduced frequency egress LNs at night, was accompanied by an increase numbers...
Abstract CaMKIIα plays an essential role in decoding Ca 2+ signaling spines by acting as a leaky integrator with the time constant of several seconds. However, mechanism which integrates signals remains elusive. Here, we imaged CaMKIIα-CaM association single dendritic using new FRET sensor and two-photon fluorescence lifetime imaging. In response to glutamate uncaging pulse, increases ~0.1 s decays over ~3 s. During repetitive uncaging, induces spine structural plasticity, did not show...
Cortical efferent and afferent fibers are arranged in a stereotyped pattern the intermediate zone (IZ). Here, we studied mechanism of axonal pathway formation by identifying molecule that is expressed subset cortical axons rat. We found T-cadherin (T-cad), member cadherin family, deep-layer cell projecting to subcortical structures, but not upper layer callosal contralateral cortex. Ectopic expression T-cad cells induced project toward structures via part IZ. Moreover, which was suppressed...
Abstract The Cre/LoxP-based conditional knockout technology is a powerful tool for gene function analysis that allows region- and time-specific manipulation. However, inserting pair of LoxP cassettes to generate can be technically challenging thus time- resource-consuming. This study proposes an efficient, low-cost method floxed mice using in vitro fertilization the CRISPR-Cas9 system over two consecutive generations. allowed us produce targeting exons 5 6 CaMK1 short period 125 days, only...
B-1 cells represent a sub-fraction of B lymphocytes that participate in T cell-independent antibody production and contribute to innate immunity. While the is favored during fetal waves lymphopoiesis, it has been unclear when how differentiation option specified. To clarify this, lymphoid hematopoietic progenitors liver (FL) adult bone marrow (ABM) were examined for cell potential. Mouse common (CLPs) more primitive KSL fraction FL ABM transferred SCID mice donor-derived subsets analyzed 4...
Regulation of gene transcription is essential for long-term plasticity in the brain. However, it has not been possible to monitor dynamics neuronal vivo and correlate them activity patterns at cellular resolution. Here we present a technique image signaling cAMP response element binding protein (CREB), an activity-dependent factor important synaptic plasticity, using 2-photon fluorescence lifetime imaging (2pFLIM) combination with new FRET biosensors. This allowed simultaneous CREB cortical...
Abstract The Cre/Loxp-based conditional knockout technology is a powerful tool for gene function analyses by allowing region-time-specific manipulation. However, inserting pair of LoxP cassettes generating knock-out can be technically challenging and thus time- resource-consuming. This study proposes an efficient, low-cost method to generate floxed mice using the in vitro fertilization CRISPR-Cas9 system over two consecutive generations. allowed us produce targeting exon 5 6 CaMK1 short...