A5091483211- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Esophageal Cancer Research and Treatment
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Coral and Marine Ecosystems Studies
- Mesenchymal stem cell research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Abdominal Trauma and Injuries
- Cancer, Stress, Anesthesia, and Immune Response
- Ocean Acidification Effects and Responses
- Marine Bivalve and Aquaculture Studies
- Lymphoma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Breast Cancer Treatment Studies
- Cancer, Hypoxia, and Metabolism
- Hedgehog Signaling Pathway Studies
- Lung Cancer Treatments and Mutations
- Genetic factors in colorectal cancer
- CAR-T cell therapy research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Hippo pathway signaling and YAP/TAZ
- Microfluidic and Bio-sensing Technologies
- FinTech, Crowdfunding, Digital Finance
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2022
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2014-2022
University of Bologna
2013-2020
Abstract Epithelial mesenchymal transition (EMT) is a physiological process necessary to normal embryologic development. However in genesis of pathological situations, this can be perverted and signaling pathways have different regulations from those physiology. In cancer invasion, such mechanism leads generation circulating tumor cells. cells become motile able shed the primary enter blood circulation. This major part invasive way cancer. EMT also implicated chronic diseases like fibrosis...
Abstract High p CO 2 habitats and their populations provide an unparalleled opportunity to assess how species may survive under future ocean acidification conditions, help reveal the traits that confer tolerance. Here we utilize a unique vent system study effects of exposure elevated CO2 on trait‐shifts observed throughout natural Astroides calycularis , azooxanthellate scleractinian coral endemic Mediterranean. Unexpected shifts in skeletal growth patterns were found. Colonies shifted...
Circulating tumor cells (CTCs) are a rare population of representing key player in the metastatic cascade. They recognized as validated tool for identification patients with higher risk relapse, including those diagnosed breast cancer (BC). However, CTCs characterized by high levels heterogeneity that also involve copy number alterations (CNAs), structural variations associated gene dosage changes. In this study, single were isolated from peripheral blood 11 early-stage BC at different time...
Epithelial-to-mesenchymal transition (EMT) has been shown to be associated with tumor progression and metastasis. During this process in breast cancer, a crucial role is played by alternative splicing systems. To identify new early prognostic marker of metastasis, we evaluated EMT-related gene expression cell lines, primary tissue from 31 patients focusing our attention on factors ESRP1, ESRP2 RBFOX2. Results showed that the patterns these genes were indicative onset EMT in-vitro models, but...
Circulating tumor cells' (CTCs) heterogeneity contributes to counteract their introduction in clinical practice. Through single-cell sequencing we aim at exploring CTC metastatic breast cancer (MBC) patients. Single CTCs were isolated using DEPArray NxT. After whole genome amplification, libraries prepared for copy number aberration (CNA) and single nucleotide variant (SNV) analysis sequenced Ion GeneStudio S5 Illumina MiSeq, respectively. demonstrate distinctive mutational signatures but...
OPINION article Front. Oncol., 11 January 2017Sec. Molecular and Cellular Oncology Volume 6 - 2016 | https://doi.org/10.3389/fonc.2016.00275
Abstract Locally advanced esophageal cancer (EC) is an aggressive disease with a dismal prognosis. The role of circulating tumor cells (CTC) in the EC metastatic process still unaddressed question. Here we monitored evolution CTCs spread 11 patients affected by locally who were undergoing neoadjuvant chemo-radio therapy followed surgery. We developed ad hoc assay named ‘Grab-all’ using DEPArray system. Longitudinal monitoring allowed identification expressing epithelial, mesenchymal or mixed...
Here, we monitored the evolution of CTCs spread in 11 patients affected by locally advanced EC who were undergoing therapy.In this perspective study, designed multiple blood biopsies from individual patients: before and after neoadjuvant chemo-radio therapy surgery. We developed a multi-target array, named Grab-all assay, to estimate for their epithelial (EpCAM/E-Cadherin/Cytokeratins) mesenchymal/stem (N-Cadherin/CD44v6/ABCG2) phenotypes. Identified isolated as single cells DEPArray,...
e22028 Background: To date, studies conducted on circulating tumor cells (CTCs) and early breast cancer (EBC) have shown a CTC-positivity rate ranging from 9.4% to 48.6%. Although correlated with worse OS DFS, CTCs were mainly found be independent of other features, such as grade receptor status. Methods: We investigated in the peripheral blood (PB) 48 stage I - II EBC patients pre- one month post-surgery using new method incorporating di-electrophoresis-based device (DEPArray). After CTC...