A5082293725- Ubiquitin and proteasome pathways
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Cancer-related Molecular Pathways
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Cardiac Arrest and Resuscitation
- Adenosine and Purinergic Signaling
- Traumatic Brain Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced Fluorescence Microscopy Techniques
- EEG and Brain-Computer Interfaces
- Ultrasonics and Acoustic Wave Propagation
- Metabolism and Genetic Disorders
- Psychosomatic Disorders and Their Treatments
- Advanced Computing and Algorithms
- Memory and Neural Mechanisms
- Neuropeptides and Animal Physiology
- Non-Destructive Testing Techniques
- Protein Degradation and Inhibitors
Yale University
2016-2024
University of Minnesota
2024
John B. Pierce Laboratory
2018-2024
Virginia Commonwealth University
2018-2019
Max Planck Society
2014
Max Planck Institute of Experimental Medicine
2012
University of Leicester
2008
MRC Toxicology Unit
2008
McMaster University Medical Centre
1980
Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current lack requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green red sensors, Ace-mNeon2 VARNAM2, their reverse response-polarity variants pAce pAceR. Our enable 0.4- 1-kilohertz recordings from >50 spiking neurons per field of view awake mice ~30-minute continuous imaging flies. Using...
In dynamic environments, animals make behavioural decisions on the basis of innate valences sensory cues and information learnt about these across multiple timescales
Effects of stimulation intramural nerves in the circular smooth muscle layer body oesophagus opossum (Didelphis marsupialis) were studied, simultaneously measuring membrane potential cells using sucrose‐gap technique and contractions muscle. Electrical field preparation, superfused with Krebs solution at 27 degrees C, induced a transient hyperpolarization cell (i.j.p.) followed by depolarization on which action potentials often superimposed. The did not develop active tension spontaneously;...
The role of GABAergic signaling in establishing a critical period for experience visual cortex is well understood. However, the effects early on synapses themselves are less clear. Here, we show that monocular deprivation (MD) during adolescent produces marked enhancement layer 2/3 mouse cortex. This coincides with weakening glutamatergic inputs, resulting significant reduction ratio excitation to inhibition. potentiation transmission arises from both an increased number inhibitory and...
Axon growth is an essential event during brain development and extremely limited due to extrinsic intrinsic inhibition in the adult brain. The E3 ubiquitin ligase Cdh1-anaphase promoting complex (APC) has emerged as important suppressor of axon growth. In this study, we identify rodents Smurf1 a novel substrate Cdh1-APC that Cdh1 targets for degradation destruction box-dependent manner. We find acts downstream turnover RhoA by process. addition, demonstrate acute knockdown vivo developing...
Axon growth is an essential process during brain development. The E3 ubiquitin ligase Cdh1-APC has emerged as a critical regulator of intrinsic axon control. Here, we identified the RhoGAP p250GAP novel interactor and found that promotes downstream Cdh1-APC. We also report undergoes non-proteolytic ubiquitination associates with Cdh1 substrate Smurf1 to synergistically regulate growth. Finally, in vivo knockdown developing cerebellar cortex results impaired migration axonal Taken together,...
<title>Abstract</title> To survive in dynamic environments, animals make behavioral decisions based on innate and learnt information about the valences of sensory cues. In brains multiple species, valence signals are initially encoded by distinct neural populations1–7 but then reconverge downstream brain structures that guide choices6–9. This reconvergence hinges prior acquisition information, which turn might depend upon signals. However, it remains unknown whether how cues shape...
Purpose: Standard physical, neurologic, and neuropsychologic examinations may not detect abnormalities after mild traumatic brain injury (mTBI). An analysis of eye movements be more sensitive to neurologic dysfunction. Methods: We performed tracking assessments in 71 active duty veteran military personnel with persistent postconcussive symptoms (3 months 5 years mTBI) 75 volunteers no history injury. Both eyes were sampled at 500 Hz analyzed for various measurement parameters during visual...
The cerebellum is crucial for sensorimotor coordination. cerebellar architecture not only requires proper development but also long-term integrity to ensure accurate functioning. Developmental defects such as impaired neuronal migration or neurodegeneration are thus detrimental the and can result in movement disorders including ataxias. In this study, we identify FBXO41 a novel CNS-specific F-box protein that localizes centrosome cytoplasm of neurons demonstrate cytoplasmic promotes...
Abstract Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between distinct, targeted cell populations. However, current indicator families lack requisite sensitivity for in vivo multipopulation imaging. We describe high-performance green red sensors, Ace-mNeon2 VARNAM2, their reverse response-polarity variants, pAce pAceR. Our enable 0.4-1 kHz recordings from >50 neurons per field-of-view awake mice ∼30-min...
Eye movements may offer a sensitive method to measure response intervention in mild traumatic brain injury (mTBI).The Brain Injury and Mechanisms of Action Hyperbaric Oxygen for Persistent Post-Concussive Symptoms after Mild Traumatic Study (BIMA) randomized 71 participants 40 sessions hyperbaric oxygen or sham. A companion normative study (Normal) enrolled 75 participants. An eye tracking system measured left right saccadic smooth pursuit. At baseline two pursuit tasks, circular horizontal...
Fluorescent genetically encoded voltage indicators report transmembrane potentials of targeted cell-types. However, voltage-imaging instrumentation has lacked the sensitivity to track spontaneous or evoked high-frequency oscillations in neural populations. Here we describe two complementary TEMPO voltage-sensing technologies that capture up ~100 Hz. Fiber-optic achieves ~10-fold greater than prior photometry systems, allows hour-long recordings, and monitors neuron-classes per fiber-optic...
Axon growth is an essential event during brain development and extremely limited due to extrinsic intrinsic inhibition in the adult brain. The E3 ubiquitin ligase Cdh1-anaphase promoting complex (APC) has emerged as important suppressor of axon growth. In this study, we identify rodents Smurf1 a novel substrate Cdh1-APC that Cdh1 targets for degradation destruction box-dependent manner. We find acts downstream turnover RhoA by process. addition, demonstrate acute knockdown vivo developing...