A5100360882- Pancreatic function and diabetes
- Diabetes Management and Research
- Hearing, Cochlea, Tinnitus, Genetics
- Diabetes and associated disorders
- Hearing Loss and Rehabilitation
- Neonatal Respiratory Health Research
- Noise Effects and Management
- Congenital Diaphragmatic Hernia Studies
- Stress Responses and Cortisol
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Cannabis and Cannabinoid Research
- Autophagy in Disease and Therapy
- Energy Harvesting in Wireless Networks
- Biochemical Analysis and Sensing Techniques
- Intraocular Surgery and Lenses
- Sirtuins and Resveratrol in Medicine
- Respiratory viral infections research
- Liver physiology and pathology
- Muscle Physiology and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ethics and Legal Issues in Pediatric Healthcare
- Regulation of Appetite and Obesity
- Mesenchymal stem cell research
- Retinal and Macular Surgery
Korea Food Research Institute
2012-2025
Korea National Institute of Health
2022-2025
Jeonbuk National University
2024
Bank of Korea
2023
Korea Disease Control and Prevention Agency
2022
Seoul St. Mary's Hospital
2022
St. Paul's Hospital
2022
Catholic University of Korea
2003-2021
National Police Hospital
2017
Within the human lung, interactions between alveolar epithelial cells and resident macrophages shape lung development function in both health disease. To study these processes, we develop a co-culture system combining pluripotent stem cell-derived organoids induced to create functional environment, termed assembloids. Using single-cell RNA sequencing analyses, identify type 2-like producing GM-CSF, which supports macrophage tissue adaptation, macrophage-like that secrete interleukin-1β...
Abstract We have reported that autophagy is crucial for clearance of amyloidogenic human IAPP (hIAPP) oligomer, suggesting an enhancer could be a therapeutic modality against diabetes with amyloid accumulation. Here, we show recently identified (MSL-7) reduces hIAPP oligomer accumulation in induced pluripotent stem cell-derived β-cells (hiPSC-β-cells) and diminishes oligomer-mediated apoptosis β-cells. Protective effects MSL-7 β-cell death are significantly reduced cells knockout MiTF/TFE...
Background Clinical application of encapsulated islet transplantation is hindered by low biocompatibility capsules leading to pericapsular fibrosis and decreased viability. To improve biocompatibility, we designed a novel chitosan-coated alginate compared them uncoated capsules. Methods Alginate were formed crosslinking with BaCl2, then they suspended in chitosan solution for 10 minutes at pH 4.5. Xenogeneic transplantation, using porcine islets 1,3-galactosyltransferase knockout mice,...
Patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (FLUAV) coinfections were associated failure more deaths. Here, we developed a model for studying SARS-CoV-2 FLUAV coinfection using human pluripotent stem cell-induced alveolar type II organoids (hiAT2). hiAT2 susceptible to infection by both viruses had features of lung damage. single markedly enhanced the susceptibility other infections. delta variants upregulated α-2-3-linked sialic acid,...
Conclusion Moderate hearing loss in young mice caused decreases cognition associated with spatial working and recognition memories 6 months. These results provide evidence for a causal relationship between cognitive impairment. Objectives Hypothesized mechanisms to connect sensory functions include the sensory-deprivation, information-degradation, common-cause hypotheses. This study intended investigate effect of on function, as estimated by radial arm maze (RAM) novel object (NOR) tasks...
ABSTRACT Background Sarcopenia, characterized by a gradual decline in skeletal muscle mass and function with age, significantly impacts both quality of life mortality. Autophagy plays crucial role maintaining health. There is growing interest leveraging autophagy to mitigate ageing effects. The impact natural activators on remains elusive. This study aims identify assess their effects ageing. Methods To discover novel activators, we screened 493 products identified Castanea crenata flower...
For alternative sources of β cells, patient-specific induced pluripotent stem cells (iPSCs) could be promising, as derived from the "self" allow autologous transplantation. However, only a few studies have investigated insulin-producing (IPCs) using iPSCs patients with type 1 diabetes (T1D). In this study, we generated IPCs T1D and 2 (T2D) compared them non-diabetic (ND) individual. To facilitate differentiation human into IPCs, PDX-1 gene expression Ad-PDX-1/VP16. T1D- T2D-specific...
Background The microencapsulation is an ideal solution to overcome immune rejection without immunosuppressive treatment. Poor biocompatibility and small molecular antigens secreted from encapsulated islets induce fibrosis infiltration. Therefore, the aims of this study were improve microcapsules by dexamethasone coating verify its effect after xenogeneic transplantation in a streptozotocin-induced diabetes mice. Methods Dexamethasone 21-phosphate (Dexa) was dissolved 1% chitosan cross-linked...
Objectives This study explores the connection between chronic stress and tinnitus, a phantom auditory perception, using an animal model. Methods Rats were subjected to 2 h of daily restraint for 10 days. Tinnitus was assessed on last day exposure gap response pre‐pulse inhibition acoustic reflex, measured at 60 dB background sound level 8, 16, 20 kHz. Chronic stress‐exposed rats categorized into two groups: tinnitus (RTG) non‐tinnitus (RNTG). Various tests, including hearing assessments...
PDX1 plays a crucial role in the development and maintenance of β-cells directly regulates pancreatic β-cell-specific transcription factors by binding to insulin gene. Here, we introduced an EGFP reporter into C-terminus KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The had normal karyotype, expressed several pluripotency markers, maintained their differentiation potential. KSCBi005-A-3 can be used monitor expression live during...
Objectives/Hypothesis Tinnitus can develop due to, or be aggravated by, stress in a rat model. To investigate as possible causal factor the development of tinnitus, we designed an animal study that included tinnitus behavior and excitatory/inhibitory neurotransmitter expression after noise exposure well restraint stress. Study Design An experimental study. Methods Wistar rats were grouped according to single double The (NE) group was subjected 110 dB sound pressure level (SPL) 16 kHz...
Pancreatic islet transplantation is a physiologically advantageous and minimally invasive procedure for the treatment of type 1 diabetes mellitus.Here, we describe first reported case successful allogeneic alone, using single-donor, marginal-dose islets in Korean patient.A 59-yr-old patient with mellitus, who suffered from recurrent severe hypoglycemia, received 4,163 equivalents/kg single brain-death donor.Isolated were infused intraportally without any complications.The immunosuppressive...
Background: Neonatal porcine pancreatic cell clusters (NPCCs) have been proposed as an alternative source of β cells for islet transplantation because their low cost and growth potential after transplantation. However, the delayed glucose lowering effect due to immaturity NPCCs immunologic rejection remain a barrier NPCC’s clinical application. Here, we demonstrate accelerated differentiation immune-tolerant by <i>in vitro</i> chemical treatment microencapsulation.Methods:...
Mature liver organoids are promising cell sources for research to understand the pathology underlying a variety of conditions affecting liver, including end-stage chronic disease. Although several methods exist differentiation mature hepatic derived from human induced pluripotent stem cells (hiPSCs), organoid generation can fail due various experimental culture conditions. Therefore, we established standard operating protocol generating and expandable hiPSCs, made starting materials...
미세각막절개 양손 수정체유화술에서 말림가능 인공수정체 삽입 후의 임상결과 이진해․이현수․김민정․박신혜․주천기 가톨릭대학교 의과대학 안과 및 시과학교실 목적: 수정체유화술 후 말아서 삽입한 ThinOptX의 임상성적을 알아보고자 한다.대상과 방법: 2005년 7월부터 2006년 5월 사이에 수술 받은 30명 34안에 대해
Intraportal pancreatic islet transplantation incurs huge cell losses during its early stages due to instant blood-mediated inflammatory reactions (IBMIRs), which may also drive regulation of the adaptive immune system. Therefore, a method that evades IBMIR will improve clinical transplantation. We used layer-by-layer approach shield non-human primate (NHP) islets with polyethylene glycol (nano-shielded islets, NSIs) and plus heparin (heparin nano-shielded islets; HNSIs). Islets ranging from...
The chronic stress has been known to have profound effect on cognition and behavior of human animal. Noise is one the most common stresses encounters in daily life. Inhibitor phosphodiesterase (PDE5) sildenafil modulates cell signaling neuroplasticity by elevating cGMP. In present study, effects memory neuroprotection noise-induced animal model were investigated. Mice exposed noise for 4 hours every day up 14 days at 110dB. They divided into following four groups; non-exposed non-treated...