Despite high anti-HBV efficacies, while the nucleoside analogs (e.g., lamivudine) lead to emergence of drug-resistance, interferons IFN-α causes adverse side-effects. Comparatively, various natural or plant products have shown similar even better efficacy. Hence, new antiviral strategies must focus not only on synthetic molecules but also potential compounds. In this report, we combined in vitro cell culture and silico molecular docking methods assess novel activity delineate inhibitory...

The human CST complex (CTC1-STN1-TEN1) is associated with telomere functions including genome stability. We have systemically analyzed the sequence of STN and performed structure analysis to establish its association Coat Plus (CP) syndrome. Many deleterious non-synonymous SNPs been identified subjected for find their pathogenic aggregation propensity. A 100-ns all-atom molecular dynamics simulation WT, R135T, D157Y structures revealed significant conformational changes in case mutants....

In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, temperature (S1), time (S2), liquid solid ratio (S3), using response surface methodology (RSM). A Box-Behnken design was used for experimental phytoconstituent analysis performed high-performance chromatography-UV. optimal found be a 64.2 °C temperature, 52.1 min time, 25.2 mL/g...

The goal of this investigation is to improve the topical delivery medicine by preparing and maximizing potential a nanotransferosome gel infused with Solanum xanthocarpum methanolic extract (SXE) provide localized regulated distribution. Thin-film hydration was used create SXE-infused nanotransferosomes (SXE-NTFs), Box–Behnken design them. Phospholipon 90G (X1), cholesterol (X2) sodium cholate (X3) were chosen as independent variables, their effects on vesicle size (Y1), polydispersity index...

Context: Guiera senegalensis J.F. Gmel (Combretaceae) is a folk medicinal plant used in various metabolic and infectious diseases. In addition to its antiviral activities against herpes fowlpox, the anti-HBV efficacy very recently reported.Objective: To develop validate simple, sensitive RP-/NP-HPTLC methods for quantitative determination of biomarkers rutin, quercetin, naringenin, gallic acid active G. leaves ethanol-extract.Materials methods: RP-HPTLC (rutin & quercetin; phase-...

Although the approved hepatitis B virus (HBV)-polymerase inhibitors (e.g., lamivudine) often lead to drug-resistance, several natural products have shown promising efficacies. Though Aloe vera (AV) gel and its constituents are of many viruses, their anti-HBV activity still remains elusive. We therefore, tested potential AV extract anthraquinones in hepatoma cells, including molecular docking, high-performance thin layer chromatography (HPTLC), cytochrome P450 (CYP3A4) activation analyses....

Guiera senegalensis J.F. Gmel is a broad-spectrum African folk- medicinal plant, having activities against fowlpox and herpes viruses. Very recently, we have shown the anti-hepatitis B vius (HBV) potential of G. leaves extract (GSLE). Here, report antioxidative hepatoprotective efficacy GSLE, including HPTLC quantification four biomarkers known antiviral activities. In cultured liver cells (HuH7) GSLE attenuated DCFH-induced oxidative stress cytotoxicity. This was supported by in vitro DPPH...

In this study, hesperidin was loaded into a transethosome and developed employing the rotary evaporator method. The formulation optimized using Box–Behnken design (BBD). HSD-TE has spherical shape, vesicle size, polydispersity index, entrapment efficiency, zeta potential within range of 178.98 nm; PDI 0.259 with −31.14 mV % EE 89.51%, respectively. in vitro drug release shows that exhibited 81.124 ± 3.45% comparison to HSD suspension. ex vivo skin permeation showed 2-fold increase gel...

This research aimed to evaluate the efficacy of a nanoemulgel (NE) containing Cananga odorata (Ylang-Ylang) oil for managing scalp psoriasis and dandruff through various assessments. The study involved phytochemical screening, characterization, stability testing, in vivo performance evaluation, dermatokinetic analysis, central composite rotatable design (CCRD) optimization, vitro release profiling, antioxidant antimicrobial activity assessment NE. NE exhibited excellent maintained physical...

The present study demonstrates the miquelianin or quercetin 3-O-glucuronide (compound 1) isolated from aerial parts of Euphorbia schimperi exhibited significant results for antioxidant and antidiabetic potential. compound 1 along with kaempferol 2) 3-O-rhamnoside 3) same source were quantified by validated HPTLC method. Antioxidant activity was determined chemical means in terms ABTS radical cation DPPH scavenging activity. Compound showed both assays as compared to standard BHA. In method...

Parthenolide, a strong cytotoxic compound found in different parts of Tarchonanthus camphoratus which motivated the authors to develop an optimized microwave-assisted extraction (MEA) method using Box–Behnken design (BBD) for efficient parthenolide from stem T. and its validation by high-performance thin-layer chromatography (HPTLC) analysis. The parameters microwave were determined as: 51.5 °C temperature, 50.8 min time, 211 W power. A quadratic polynomial model was most suitable with R2...

The interactions among various biomarkers remained unexplored under the stressful environment of high-altitude. Present study evaluated to susceptibility for high altitude pulmonary edema (HAPE) in HAPE-patients (HAPE-p) and adaptation highland natives (HLs); both comparison HAPE-free sojourners (HAPE-f). All subjects were recruited at 3500 m. We measured clinical parameters, biochemical levels plasma gene expression using RNA from blood; analyzed correlations between especially arterial...

The genus Hibiscus contains about 275 species of flowering plants widely grown in the tropics and sub-tropics. available literature revealed that several exhibited excellent anticancer activity against cancer cells like lung, breast, liver. This motivated authors to explore property other (Hibiscus calyphyllus, H. deflersii micranthus) along with development a validated HPTLC method for concurrent analysis three biomarkers (ursolic acid, β-sitosterol lupeol) different species. various...

Abstract Protection of telomere 1 (POT1) is one the key components shelterin complex, implicated in maintaining homeostasis, and thus stability eukaryotic genome. A large number non‐synonymous single nucleotide polymorphisms (nsSNPs) POT1 gene have been reported to cause varieties human diseases, including cancer. In recent years, a mutations has markedly increased, interpreting effect these numbers understand mechanism associated diseases seems impossible using experimental approaches....

The present study assessed the comparative antioxidant potential of ethanol extract (EE) leaves four Acacia species (Acacia salicina, AS; laeta, AL; hamulosa AH; and tortilis, AT) grown in Saudi Arabia, including RP-HPTLC quantification biomarker rutin. In vitro DPPH radical scavenging β-carotene-linoleic acid bleaching assays showed promising activities extracts: ASEE (IC50: 60.39 324.65 μg/ml) >ALEE 217.06 423.36 >ATEE 250.13 747.50 >AHEE 255.83 417.28 μg/ml). This was comparable to rutin...

This study explores a novel approach to managing skin conditions through combination therapy utilizing phospholipid-enriched edge activator-based nanoformulation. 5-Fluorouracil (5-FU)- and sesamol (SES)-loaded transliposomes (FS-TL) were developed using thin film hydration method optimized Box-Behnken Design. FS-TL characterization indicated vesicle size of 165.6 ± 1.1 nm, polydispersity index 0.28 0.01, zeta potential −33.17 0.9 mV, the percent entrapment efficiencies for 5-FU SES found be...

Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), continues to be a major global health burden, particularly with the alarming rise of multi‐drug‐resistant (MDR) and extensively drug‐resistant (XDR) strains that compromise efficacy conventional therapies. Hence, we designed new set spirooxindole‐ spiroindenoquinoxaline‐derived pyrrolo[3,4‐ c ] pyrroles have been synthesized through atom economic one‐pot multicomponent reactions isatin/indenoquinoxaline phenylalanine...

Solanum xanthocarpum (SX) has been used to treat a variety of diseases, including skin disorders like psoriasis (PSO). SX possesses many pharmacological activities anti-inflammatory, anti-cancer, immunosuppressive, and healing qualities. However, the multi-target mechanism on PSO still needs clarity. Materials methods: The Indian Medicinal Plants, Phytochemicals Therapeutics (IMPPAT) database Swiss Target Prediction online tool were find active phytochemical components their associated...