A5100457745- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- interferon and immune responses
- Spinal Cord Injury Research
- Neurological Disease Mechanisms and Treatments
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Angiogenesis and VEGF in Cancer
- Olfactory and Sensory Function Studies
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Barrier Structure and Function Studies
- Genetics, Aging, and Longevity in Model Organisms
- Wound Healing and Treatments
- Biochemical Acid Research Studies
- Cerebrovascular and genetic disorders
- Child and Animal Learning Development
First Affiliated Hospital of Xi'an Jiaotong University
2025
University of Minnesota Medical Center
2025
Zhongnan Hospital of Wuhan University
2022-2025
Zhejiang University
2022-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2021-2024
Second Affiliated Hospital of Zhejiang University
2024
University of Minnesota
2022-2023
Guilin University of Electronic Technology
2023
South China Normal University
2019-2023
Peking Union Medical College Hospital
2022
The transcription factor NF-kappa B is sequestered in the cytoplasm by inhibitor protein I kappa alpha. Extracellular inducers of activate signal transduction pathways that result phosphorylation and subsequent degradation At present, link between alpha its not understood. In this report we provide evidence serine residues 32 36 targets to ubiquitin-proteasome pathway. ubiquitinated vivo vitro following phosphorylation, mutations abolish prevent ubiquitination vitro. Ubiquitinated remains...
The inhibitor protein I kappa B alpha controls the nuclear import of transcription factor NF-kappa B. inhibitory activity is regulated from cytoplasmic compartment by signal-induced proteolysis. Previous studies have shown that signal-dependent phosphorylation serine residues 32 and 36 targets to ubiquitin-proteasome pathway. Here we provide evidence lysine 21 22 serve as primary sites for ubiquitination alpha. Conservative Lys-->Arg substitutions at both Lys-21 Lys-22 produce...
Background: Alzheimer's disease (AD), one of the most common forms dementia, is a widely studied neurodegenerative characterized by Aβ accumulation and tau hyperphosphorylation. Currently, there no effective cure available for AD.The astrocyte AQP4 polarized distribution-mediated glymphatic system essential abnormal clearance potential therapeutic target AD.However, role exercise on distribution association between phenotype polarization are poorly understood.Methods: Using streptozotocin...
The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. NOVA regulate at least 700 events in vivo, yet relatively little is known about the biologic consequences of action particular functional differences between NOVA2. Transcriptome-wide searches for isoform-specific functions, using HITS-CLIP RNA-seq data from mouse cortex lacking either isoform, reveals that uniquely regulates a series axon guidance related genes during cortical...
Accessory proteins in Frizzled (FZD) receptor complexes are thought to determine ligand selectivity and signaling amplitude. Genetic evidence indicates that specific combinations of accessory ligands mediate vascular β-catenin different CNS structures. In the retina, tetraspanin TSPAN12 norrin (NDP) angiogenesis, both genes linked familial exudative vitreoretinopathy (FEVR), yet molecular function remains poorly understood. Here, we report is an essential component NDP complex interacts with...
Ischemic spinal cord injury (ISCI) results in the motor sensory dysfunction of limbs below site. In response to injury, astrocytes develop into neuroprotective [(neurotrophic reactive (A2s)] mitigate damage. MicroRNA (miR)-21 can promote development neuroinflammation previous studies. Our aim was investigate effect miR-21 on its polarization. We used abdominal aortic occlusion model vivo. Immunohistochemistry detect distribution A2s cord. an oxygen glucose deprivation method ischemia vitro...
RNA-binding proteins (RBPs) control multiple aspects of post-transcriptional gene regulation and function during various biological processes in the nervous system. To further reveal functional significance RBPs neural development, we carried out an vivo RNAi screen dorsal spinal cord interneurons, including commissural neurons. We found that NOVA family play a key role neuronal migration, axon outgrowth, guidance. Interestingly, Nova mutants display similar defects as knockout Dcc...
The aim of this study was to investigate the pro-angiogenic potential olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) possible underlying mechanisms.Vascular endothelial or tissues were cultured treated with conditioned medium (CM) extracted from OECs through addition LPS CCM unactivated controls. Concomitantly, assessed in vitro aortic ring sprouting assay, wound healing CCK-8 tube formation assay. Subsequently, co-cultured evaluate their...
Abstract— “Transfer” is a venerable issue in cognitive development and education. However, its very existence the subject of extensive debate, there as yet no consensus about definition, measurement, implications. This article proposes 3-dimensional conceptual model transfer distance for thinking concepts or strategies children, presents some recent findings on children’s scientific reasoning strategies—task similarity, context temporal interval—that exemplify these three dimensions. These...
Objective- Blood-CNS (central nervous system) barrier defects are implicated in retinopathies, neurodegenerative diseases, stroke, and epilepsy, yet, the pathological mechanisms downstream of remain incompletely understood. Blood-retina (BRB) formation retinal angiogenesis require β-catenin signaling induced by ligand norrin (NDP [Norrie disease protein]), receptor FZD4 (frizzled 4), coreceptor LRP5 (low-density lipoprotein receptor-like protein 5), tetraspanin TSPAN12 (tetraspanin 12)....
Edaravone Dexborneol (EDB), comprised of edaravone and (+)- bornel, has been demonstrated to have synergistic effects antioxidant anti-inflammatory, which makes it be applied for stroke as a protectant. However, the underlying mechanism neuroprotection EDB not fully elucidated. Increasing evidence shown that neurotoxic A1 astrocytes were closely related neuronal death after cerebral ischemia. whether could provide by modulating activation yet The present study aimed explore afforded...
Despite decades of research, senile osteoporosis still lacks effective treatments. Exercise-mediated mechanical loading enhances bone density, strength, and microstructure, while lack exercise leads to significant loss. In this study, we used treadmill tail-suspension mouse models explore the effects on metabolism. We found that osteoblast signal transduction factors activity Immediate Early Response 3 (IER3) protein aligned with exercise-induced changes in mass metabolic levels. Mechanical...
Abstract Background Robo1, Robo2 and Rig-1 (Robo3), members of the Robo protein family, are candidate receptors for chemorepellents Slit known to play a crucial role in commissural axon guidance spinal cord. However, their roles at other axial levels remain unknown. Here we examine expression proteins by cerebellofugal (CF) axons rostral hindbrain investigate CF pathfinding analysing knockout mice. Results We analysed originating from deep cerebellar neurons rodent embryos, focusing on...
Purslane is a widespread succulent herb that exhibits various pharmacological effects. The purpose of this study was to evaluate the protective effect Portulaca oleracea L. (purslane) on streptozotocin-induced diabetes in mice. Oral glucose-tolerance tests were carried out assess blood glucose levels and body weight food intake recorded. biochemical parameters anti-aspartate aminotransferase, alanine insulin, triglycerides, total cholesterol, IL-6, IL-1β, TNFα also measured. pathological...
The development of the epidermis Caenorhabditis elegans involves cell fusion, migration, and differentiation events. To understand mechanisms underlying these processes, we characterized roles NHR-25, a member nuclear receptor family transcription factors. NHR-25 homologs Ftz-F1 in Drosophila SF-1 mammals are involved various biological including regulation patterning during development, reproduction, metabolism, metamorphosis, homeostasis. Impairment nhr-25 activity leads to severe...
Abstract Angiogenesis and blood–brain barrier formation are required for normal central nervous system (CNS) function. Both processes controlled by Wnt or Norrin (NDP) ligands, Frizzled (FZD) receptors, β-catenin-dependent signalling in vascular endothelial cells. In the retina, FZD4 ligand NDP critical mediators of mutated familial exudative vitreoretinopathy. Here, we report that is a potent trigger ubiquitination induces internalization receptor complex into endo-lysosomal compartment....
Proper connectivity of the nervous system requires temporal and spatial control axon guidance signaling. As commissural axons navigate across CNS midline, ROBO-mediated repulsion has traditionally been thought to be repressed before crossing, then become upregulated after crossing. The regulation ROBO receptors involves multiple mechanisms that protein expression, trafficking, activity. Here, we report mammalian ROBO1 ROBO2 are not uniformly inhibited precrossing instead subject additional...
Based on findings in rodents, we sought to test the hypothesis that purinergic modulation of synaptic transmission occurs human intestine. Time series analysis intraneuronal free Ca(2+) levels submucosal plexus (SMP) from Roux-en-Y specimens was done using Zeiss LSM laser-scanning confocal fluo-4 AM imaging. A 3-s fiber tract stimulation (FTS) used elicit a response. Short-circuit current (I(sc) = chloride secretion) recorded mucosa-SMP flux chambers. distension reflex or electrical field...