A5036033432- Bacterial biofilms and quorum sensing
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Marine Biology and Environmental Chemistry
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Orthopedic Infections and Treatments
- Graphene and Nanomaterials Applications
- Biosensors and Analytical Detection
- Microplastics and Plastic Pollution
- Microfluidic and Capillary Electrophoresis Applications
- Bacterial Identification and Susceptibility Testing
- Pickering emulsions and particle stabilization
- Minerals Flotation and Separation Techniques
- bioluminescence and chemiluminescence research
- Cell Image Analysis Techniques
- Bone Tissue Engineering Materials
- Inhalation and Respiratory Drug Delivery
- Surface Modification and Superhydrophobicity
- Photoacoustic and Ultrasonic Imaging
- Oral microbiology and periodontitis research
- Marine Ecology and Invasive Species
- Electrostatics and Colloid Interactions
- Ichthyology and Marine Biology
University Medical Center Groningen
2016-2025
University of Groningen
2015-2024
Biology of Infection
1988-2014
Biocom
1988-2014
Bacteria in the biofilm mode of growth are protected against chemical and mechanical stresses. Biofilms composed, for most part, extracellular polymeric substances (EPSs). The matrix is composed different constituents, such as proteins, polysaccharides, DNA (eDNA). Here we aimed to identify roles constituents viscoelastic response biofilms. Staphylococcus aureus, epidermidis, Streptococcus mutans, Pseudomonas aeruginosa biofilms were grown under conditions yielding distinct chemistries....
Abstract Optical-coherence-tomography (OCT) is a non-destructive tool for biofilm imaging, not requiring staining, and used to measure thickness putative comparison of structure based on signal intensity distributions in OCT-images. Quantitative intensities OCT-images, difficult due the auto-scaling applied OCT-instruments ensure optimal quality individual images. Here, we developed method eliminate influence order allow quantitative densities different Auto- re-scaled could be qualitatively...
A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into soft hydrogel network on poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal was molecularly characterized via Coomassie and Lowry assay protein staining agents as well by X-ray photoelectron spectroscopy. activity established against Staphylococcus aureus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain...
Abstract In this article we studied the adsorption of serum albumin to substrata with a broad range wettabilities from solutions protein concentrations between 0.03 and 3.00 mg · mL −1 in parallel‐plate flow cell. Wall shear rates were varied 20 2000 s . The amount adsorbed stationary state was always highest on PTFE, most hydrophobic material employed decreased increasing wettability substrata. Increasing amounts observed wall at lowest concentration. Inverse observations made Transmission...
Deposition of oral bacteria onto glass from a potassium phosphate buffered suspension has been studied in parallel plate flow cell. Three bacterial strains with known surface free energies and zeta potentials, two Strains (S. salivarius HB S. HB‐C12) having well‐defined appendages have employed. Polystyrene latices were included to provide comparison inert particles. Initial deposition rates the various ranged 0–1—2·9 × 103 particles cm ""2 sec"; '. The initial related an approximate theory...
Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly through multiple, reversibly-binding tethers detach successively re-attach, but not collectively cause detachment of an entire bacterium. Arguments build on combining analyses confined Brownian-motion adhering glass their...
Marine biofouling has severe economic impacts and cyanobacteria play a significant role as early surface colonizers. Despite this fact, cyanobacterial biofilm formation studies in controlled hydrodynamic conditions are scarce. In work, computational fluid dynamics was used to determine the shear rate field on coupons that were placed inside wells of agitated 12-well microtiter plates. Biofilm by three different strains assessed at two rates (4 40 s-1 ) which can be found natural ecosystems...
In vivo evaluations of the antimicrobial efficacy biomaterials often use bioluminescent imaging modalities based on bacteria to allow follow-up in single animals. Bioluminescence production by is dependent their metabolic activity. It well known that several factors can influence metabolism bacteria, such as antimicrobials and changes bacterial growth phase. However, little about intracellular residence bioluminescence. For example, Staphylococcus aureus survive peri-implant tissue...
Models for bacterial adhesion to substratum surfaces all include uncertainty with respect the (ir)reversibility of adhesion. In a model, based on vibrations exhibited by adhering bacteria parallel surface, was described as result reversible binding multiple tethers that detach from and successively reattach eventually making irreversible. Here, we use total internal reflection microscopy determine whether also exhibit variations over time in their perpendicular distance above surfaces....
Abstract Staphylococcus aureus is commonly associated with biofilm-related infections and contributes to the large financial loss that accompany nosocomial infections. The micrococcal nuclease Nuc1 enzyme limits biofilm formation via cleavage of eDNA, a structural component matrix. Solid surface hydrophobicity influences bacterial adhesion forces may as well influence eDNA production. Therefore, it hypothesized impact activity dependent on characteristics solid surfaces. For this reason,...
platforms capable of mimicking the hydrodynamic conditions prevailing in natural aquatic environments have been previously validated and used to predict fouling behavior on different surfaces. Computational Fluid Dynamics (CFD) has shear forces occurring these platforms. In general, predictions are made for initial stages biofilm formation, where amount does not affect flow behavior, enabling estimation that adhering organisms withstand. this work, we go a step further understanding when...
Bacteria can be dead, alive, or exhibit slowed suspended life forms, making bacterial death difficult to establish. Here, agar-plating, microscopic-counting, SYTO9/propidium-iodide staining, MTT-conversion, and bioluminescence-imaging were used determine upon exposure different conditions. Rank correlations between pairs of assay outcomes low, indicating assays measure aspects death. Principal-component analysis yielded two principal components, named "reproductive-ability" (PC1)...