Extracellular DNA (eDNA) is an important component of the biofilm matrix. We show that removal eDNA from Gram-positive bacteria reduces initial adhesion to and aggregation on surfaces. Thermodynamic analyses indicated introduces favorable acid-base interactions, explaining effect surface.

Abstract Biofilms are detrimental in many industrial and biomedical applications prevention of biofilm formation has been a prime challenge for decades. consist communities adhering bacteria, supported protected by extracellular‐polymeric‐substances (EPS), the so‐called “house organisms”. EPS consists water, proteins, polysaccharides extracellular‐DNA (eDNA). eDNA, being longest molecule EPS, connects different components therewith holds an together. eDNA is associated with bacterial cell...

Centrifugal damage has been known to alter bacterial cell surface properties and interior structures, including DNA. Very few studies exist on caused by centrifugation because of the difficulty in relating speed container geometry caused. Here, we provide a simple, versatile method analysis for describing compaction bacteria during based proposed coefficient, C. Values C can be related different properties. Changing or speeds changed value significantly. Initial deposition rates...

Chronic lung diseases such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive disease (COPD) are associated with changes in extracellular matrix (ECM) composition abundance affecting the mechanical properties of lung. This study aimed to generate ECM hydrogels from control, severe COPD [Global Initiative for Obstructive Lung Disease (GOLD) IV], fibrotic human tissue evaluate whether their stiffness viscoelastic were reflective native tissue. For hydrogel generation, GOLD IV,...

Bacteria in the biofilm mode of growth are protected against chemical and mechanical stresses. Biofilms composed, for most part, extracellular polymeric substances (EPSs). The matrix is composed different constituents, such as proteins, polysaccharides, DNA (eDNA). Here we aimed to identify roles constituents viscoelastic response biofilms. Staphylococcus aureus, epidermidis, Streptococcus mutans, Pseudomonas aeruginosa biofilms were grown under conditions yielding distinct chemistries....

Root canal treatment addresses infectious processes that require control. Occasionally, the radicular pulp is vital and inflamed, presenting a superficial infection. To preserve pulpal remnants, conservative procedures have gained favor, employing anti-inflammatory medications. This study investigated effects of propolis (PRO), copaiba oil-resin (COR) associated with hydrocortisone (H) compared their impact to Otosporin® concerning cytotoxic genotoxic activity, cytokine detection, toxicity...

The dynamic magnetic properties of powdered ``x-ray amorphous'' ${\mathrm{Fe}}_{2}{\mathrm{O}}_{3}$ sample with mean particle size $\ensuremath{\sim}12.0\phantom{\rule{0.3em}{0ex}}\mathrm{nm}$ $(\ensuremath{\sigma}=\ifmmode\pm\else\textpm\fi{}0.36)$ have been investigated using frequency dependent real part ac susceptibility ${\ensuremath{\chi}}_{\mathrm{ac}}^{\ensuremath{'}}$ and time dc magnetization studies. A frequency-dependent peak was observed in vs $T$ measurements. dependence the...

Triblock copolymers of polyethylene oxide (PEO) and polypropylene (PPO), that is, PEOn-PPOm-PEOn, better known as Pluronic can adsorb to surfaces in either a pancake or brushlike configuration. The configuration is advantageous numerous applications, since it constitutes surface repellent proteins microorganisms. conformation the adsorbed layer depends on hydrophobicity substratum surface, but threshold above which adopted unknown. Therefore, aim this study investigate F-127 adsorption with...

Extracellular DNA (eDNA) plays a significant role in bacterial biofilm formation and aggregation. Here, for the first time, we present physico-chemical analysis of DNA-mediated aggregation three strains (Streptococcus mutansLT11, Pseudomonas aeruginosaPAO1 Staphylococcus epidermidis 1457). Adsorption on cell surfaces increased with increasing concentration, reaching level around 4–6 × 10−9 μg per bacterium, concurrent maximal Further increase adsorption caused decrease Water contact angles...

The aim of this study was to investigate the role extracellular DNA (eDNA) on adhesion strength Streptococcus mutans LT11 substrata with different hydrophobicities at high and low ionic strengths. AFM forces a hydrophilic hydrophobic substratum increased increasing surface-delay times were stronger than substratum. presence eDNA streptococcal cell surface enhanced its force significantly more substratum, especially after bond maturation. Bond maturation accompanied by an number minor peaks,...

Wear resistant and ultralow friction in synovial joints is the outcome of a sophisticated synergy between major macromolecules fluid, e.g., hyaluronan (HA) proteoglycan 4 (PRG4), with collagen type II fibrils other non-collagenous cartilage superficial zone (SZ). This study aimed at better understanding mechanism PRG4 localization surface. We show direct interactions surface bound HA freely floating using quartz crystal microbalance dissipation (QCM-D). Freely was also shown to bind fibrils....

In a quartz crystal microbalance, particles adhering to sensor are perturbed around their equilibrium positions via thickness-shear vibrations at the crystal's fundamental frequency and overtones. The amount of adsorbed molecular mass is measured as shift in resonance frequency. inertial loading, shifts negative proportional mass, contrast with "elastic loading", where adhere small contact points. Elastic loading air yields positive according coupled model. We explore here novel application...

Antimicrobial peptides, also known as host defense have recently emerged a promising new category of therapeutic agents for the treatment infectious diseases. This study evaluated preclinical in vitro, ex vivo, and vivo antimicrobial activity, well potential to cause skin irritation, human kininogen-derived peptide DPK-060 different formulations designed topical delivery. We found that formulated acetate buffer or poloxamer gel caused marked reduction bacterial counts Staphylococcus aureus...

Fat grafting is an established clinical intervention to promote tissue repair. The role of the fat's extracellular matrix (ECM) in regeneration largely neglected. We investigated vitro use human adipose tissue-derived ECM hydrogels as release platform for factors secreted by adipose-derived stromal cells (ASCs). Lipoaspirates from nondiabetic and diabetic donors were decellularized. Finely powdered acellular was evaluated cell remainders DNA content. Acellular digested, formed at 37°C their...

Abstract Aim To investigate the anti‐biofilm efficacy of irrigation using a simulated root canal model, chemical effect irrigants against biofilms grown on dentine discs and their impact biofilm viscoelasticity, in decontaminating infected dentinal tubules capacity bacteria to regrow. Methodology Biofilm removal, viscoelastic analysis remaining bacterial viability were evaluated model with lateral morphological features, tubule respectively. Experiments conducted two‐phase protocol. Phase 1:...

Due to its high molecular weight and viscosity, hyaluronic acid (HA) is widely used for viscosupplementation provide joint pain relief in osteoarthritis. However, this benefit temporary due poor adhesion of HA on articular surfaces. In study, we therefore conjugated with dopamine form HADN, which made the adhesive while retaining viscosity enhancement capacity. We hypothesized that HADN could enhance cartilage lubrication through adsorption onto exposed collagen type II network repair lamina...

Extracellular matrix (ECM) is a dynamic network of proteins, proteoglycans and glycosaminoglycans, providing structure to the tissue biochemical biomechanical instructions resident cells. In fibrosis, composition organization ECM are altered, these changes influence cellular behaviour. Biochemical (i. e. protein composition) in take place simultaneously vivo. Investigating individually vitro examine their (patho)physiological effects has been difficult. this study, we generated an model...