A5052940305- Protein purification and stability
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Polymer Surface Interaction Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Safe Handling of Antineoplastic Drugs
- Orthopedic Infections and Treatments
- Antimicrobial agents and applications
- Biosimilars and Bioanalytical Methods
- Dental materials and restorations
- Transgenic Plants and Applications
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Pharmaceutical studies and practices
- Nanofabrication and Lithography Techniques
- Coagulation and Flocculation Studies
- HER2/EGFR in Cancer Research
- Antimicrobial Peptides and Activities
- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- Electrostatics and Colloid Interactions
- Cell Adhesion Molecules Research
- Drug Solubulity and Delivery Systems
- Analytical Chemistry and Chromatography
- Wound Healing and Treatments
University of Iowa
2022-2025
Centre for Human Drug Research
2016-2018
Leiden University
2016-2018
University of Groningen
2007-2012
University Medical Center Groningen
2007-2012
The University of Texas at San Antonio
2010
Triblock copolymers of polyethylene oxide (PEO) and polypropylene (PPO), that is, PEOn-PPOm-PEOn, better known as Pluronic can adsorb to surfaces in either a pancake or brushlike configuration. The configuration is advantageous numerous applications, since it constitutes surface repellent proteins microorganisms. conformation the adsorbed layer depends on hydrophobicity substratum surface, but threshold above which adopted unknown. Therefore, aim this study investigate F-127 adsorption with...
We introduce a procedure for determining shear forces at the balance between attachment and detachment of bacteria under flow. This can be applied to derive adhesion in weak-adherence systems, such as polymer brush coatings, which are currently center attention their control bacterial biofilm formation.
Biofilms causing biomaterial-associated infection resist antibiotic treatment and usually necessitate the replacement of infected implants. Here we relate bacterial adhesion forces susceptibility biofilms on uncoated polymer brush-coated silicone rubber. Nine strains Staphylococcus aureus, epidermidis, Pseudomonas aeruginosa adhered more weakly to rubber (-0.05 ± 0.03 -0.51 0.62 nN) than (-1.05 0.46 -5.1 1.3 nN). adhering organisms brush coatings remained in a planktonic state, susceptible...
The fate of secondary biomaterial implants was determined by bio-optical imaging and plate counting, after antibiotic treatment biomaterials-associated-infection (BAI) surgical removal an experimentally infected, primary implant. All tissue samples from control mice showed bioluminescence were culture-positive. In treated group, no detected only 20% all After revision surgery, in mice. the 80% contrast, 17% 33% culture-positive, despite treatment. study illustrates that due to BAI a implant,...
The foundation of a biosimilar manufacturer's regulatory filing is the demonstration analytical and functional similarity between product pertinent originator product. excipients in formulation may interfere with characterization using typical techniques during this biosimilarity exercise. Consequently, producers products resort to buffer exchange isolate biotherapeutic protein from drug formulation. However, impact that isolation has on stability not completely known. This study aims...
To measure aggregate and particle formation in tumor necrosis factor-alpha (TNF-α) inhibitors etanercept, adalimumab certolizumab pegol product samples after exposure to freezing temperature conditions similar storage previously observed patients' homes. TNF-α their original primary secondary packaging were exposed 32 freeze-thaw cycles (−10°C for 120min/5°C 60 min) or continuous low (−20°C 96 h) before thawing at 2–8°C. Non-stressed products used as controls. The analyzed by high pressure...
Curing biomaterial-associated infection (BAI) frequently includes antibiotic treatment, implant removal and re-implantation. However, revision implants are at a greater risk of as they may attract bacteria from their infected surroundings. Polymer brush-coatings low numbers bacteria, but the virtue polymer in vivo has seldom been investigated. Here, we determine possible benefits brush-coated versus pristine silicone rubber surgery, using murine model. BAI was induced 26 mice by subcutaneous...
Despite the increasing availability and success of monoclonal antibodies (mAb), early identification candidate molecules with desirable developability attributes remains challenging due to self-association poor solution behavior. Measuring these phenomena experimentally using available methods is complicated in mAbs development. Quartz crystal microbalance dissipation monitoring (QCM-D) detects a loosely interacting layer on top irreversibly adsorbed molecules, providing information about...