A5085462138- Metabolomics and Mass Spectrometry Studies
- Sugarcane Cultivation and Processing
- Spectroscopy and Chemometric Analyses
- Natural Products and Biological Research
- Photochemistry and Electron Transfer Studies
- Protein purification and stability
- Genetic Mapping and Diversity in Plants and Animals
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- Diet, Metabolism, and Disease
- Advanced MRI Techniques and Applications
- NMR spectroscopy and applications
- Molecular Junctions and Nanostructures
- Advanced Chemical Sensor Technologies
- Molecular Sensors and Ion Detection
- Advanced Proteomics Techniques and Applications
- Microscopic Colitis
- Porphyrin and Phthalocyanine Chemistry
- Diabetes Treatment and Management
- Diet and metabolism studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiovascular Function and Risk Factors
- Metabolism, Diabetes, and Cancer
Novo Nordisk (Denmark)
2004-2023
Imperial College London
2011
Royal Agricultural University
2000
University of Copenhagen
1999
We have performed a metabolite quantitative trait locus (mQTL) study of the (1)H nuclear magnetic resonance spectroscopy ((1)H NMR) metabolome in humans, building on recent targeted knowledge genetic drivers metabolic regulation. Urine and plasma samples were collected from two cohorts individuals European descent, with one cohort comprised female twins donating longitudinally. Sample concentrations quantified by NMR tested for association genome-wide single-nucleotide polymorphisms (SNPs)....
To gain novel insight into the molecular mechanisms underlying hydrazine-induced hepatotoxicity, mRNAs, proteins and endogenous metabolites were identified that altered in rats treated with hydrazine compared untreated controls. These changes resolved a combined genomics, proteomics metabonomics study. Sprague–Dawley assigned to three treatment groups 10 animals per group given single oral dose of vehicle, 30 or 90 mg kg−1 hydrazine, respectively. RNA was extracted from rat liver 48 h...
A proteomics approach combined with multivariate data analysis was used to examine the hepatotoxic effect of hydrazine in 30 male Sprague Dawley rats, assigned four treatment groups and two control groups. Liver samples from individual animals were resolved by two-dimensional differential gel electrophoresis (2-D DIGE) protein patterns 2-D gels analyzed principal component (PCA) partial least squares regression (PLSR). The PCA plot able describe variation expression related dose time,...
Metabonomics has been defined as "quantitative measurement of the dynamic multiparametric metabolic response living systems to pathophysiological stimuli or genetic modification" and can provide information on disease processes, drug toxicity, gene function. In this approach many samples biological origin (biofluids such urine plasma) are analyzed using techniques that produce simultaneous detection. A variety analytical profiling tools used routinely, also currently under development,...
It is important to identify patients with Maturity-onset diabetes of the young (MODY) as a molecular diagnosis determines both treatment and prognosis. Genetic testing currently expensive many are therefore not assessed misclassified having either type 1 or 2 diabetes. Biomarkers could facilitate prioritisation for genetic testing. We hypothesised that different underlying aetiologies their have distinct metabolic profiles which may uncover novel biomarkers. The aim this study was perform...
In a fluorescence study of raw cane sugar samples, two-way and three-way chemometric methods have been used to extract information about the individual fluorophores in from excitation−emission landscapes. A sample set 47 samples representing varied selection was analyzed, three with (275, 350) nm, (340, 420) (390, 460) nm as their approximate excitation emission maxima were found. The spectral profiles estimated decomposition model PARAFAC. Two-way principal component analysis (PCA) unfolded...
The photophysical properties of 2,3,6,7,10,11-hexakis(n-hexylsulfanyl)triphenylene and 2,3,6,7,10,11-hexakis(n-hexylsulfonyl)triphenylene have been investigated by absorption, fluorescence phosphorescence spectroscopy compared with parent triphenylene 2,3,6,7,10,11-hexakis(n-hexyloxy)triphenylene. It is concluded that alkylsulfanyl substituents interact more strongly the core than alkylsulfonyl alkyloxy substituents, as revealed absorption emission spectra well rate constants for radiative...
A comparison was made of the fluorophores in beet thick juice and cane final evaporator syrup, which are comparable production sugar; that is, both represent stage syrup concentration prior to crystallization sugar. To further elucidate nature color components a series model colorants also prepared, consisting mildly alkaline-degraded fructose glucose two Maillard type colorants, glucose--glycine glucose--lysine. Fluorescence excitation--emission landscapes resolved into individual...
The phosphorescence spectra of triphenylene and truxene, recorded in glassy solvents at 77 K, are presented. Their vibronic structure is interpreted on the basis intensities computed with help quantum-chemical calculations. It shown to be due Herzberg−Teller-active nontotally symmetric modes which provide false origins for progressions totally modes. Similarly benzene, dominant contribution T1 → S0 transition two disklike molecules derive from triplet−triplet dipole moments. Radiative...
It has been shown that fluorescence spectroscopy of sugar in aqueous solution carries important quality and process information related to beet factories, which is accessible by multivariate analyses. A method for measuring crystalline directly on-line the should be advantageous. In this paper we compare measurement technique with two methods on solid sugar. Surprisingly, it was possible measure through crystals using same transmission techniques 90° detection as solutions. This compared a...
Introduction Advances in analytical technologies enable investigation of possible correlations between molecular structure, aggregation and subvisible particle content. Regulatory agencies place increasing attention on potential risks associated with protein aggregates the micron range biological therapeutics. Aim Assess heterogeneity, high‐molecular‐weight ( HMWP ) species, content posttranslational modifications six commercially available recombinant FVIII rFVIII products. Methods Three...
Fluorescence from sugar crystal samples has previously been used to obtain information about factory imprint and quality. Solid-phase fluorescence potential as a fast screening method, but the spectra are highly influenced by measurement geometry sample. The aim of present study was examine how measurements related crystals for better understanding both. Initially, five varied composition were sieved into size fractions. excitation-emission landscapes fractions measured with solid...
The phosphorescence spectra of triphenylene and truxene, recorded in glassy solvents at 77 K, are presented. Their vibronic structure is interpreted on the basis intensities computed with help quantum‐chemical calculations. agreement between observed simulated very satisfactory contributes to improve complete assignment ground state frequencies two disk‐like molecules.