A5081854073- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- NMR spectroscopy and applications
- Advanced MRI Techniques and Applications
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Pancreatic function and diabetes
- Advanced NMR Techniques and Applications
- Birth, Development, and Health
- Multiple Myeloma Research and Treatments
- Enzyme Structure and Function
- Muscle metabolism and nutrition
- RNA and protein synthesis mechanisms
- Oral microbiology and periodontitis research
- HIV/AIDS drug development and treatment
- Nutritional Studies and Diet
- Cancer Treatment and Pharmacology
- Diabetes Management and Research
- Traditional Chinese Medicine Studies
- Chronic Kidney Disease and Diabetes
- Dental Health and Care Utilization
- Retinopathy of Prematurity Studies
- Genetic Associations and Epidemiology
University of Gothenburg
2005-2024
National Bioinformatics Infrastructure Sweden
2023-2024
University of Copenhagen
2021
Novo Nordisk (Denmark)
2007-2012
Novo Nordisk (United Kingdom)
2008
Centre for Human Genetics
2008
Oxford Centre for Diabetes, Endocrinology and Metabolism
2008
Churchill Hospital
2008
University of Oxford
2008
Imperial College London
2008
We have performed a metabolite quantitative trait locus (mQTL) study of the (1)H nuclear magnetic resonance spectroscopy ((1)H NMR) metabolome in humans, building on recent targeted knowledge genetic drivers metabolic regulation. Urine and plasma samples were collected from two cohorts individuals European descent, with one cohort comprised female twins donating longitudinally. Sample concentrations quantified by NMR tested for association genome-wide single-nucleotide polymorphisms (SNPs)....
Aim . Determine the metabolic profile and identify risk factors of women transitioning from gestational diabetes mellitus (GDM) to type 2 (T2DM). Methods 237 diagnosed with GDM underwent an oral glucose tolerance test (OGTT), anthropometrics assessment, completed lifestyle questionnaires six years after pregnancy. Blood was analysed for clinical variables (e.g., insulin, glucose, HbA1c, adiponectin, leptin, lipid levels) NMR metabolomics. Based on OGTT, were divided into three groups: normal...
Coupling evolution periods in NMR experiments on proteins has recently attracted much attention for its substantial savings measurement time. Using the concept of multiway decomposition, which already proved useful many types applications, novel tool PRODECOMP decomposes sets spectra with coupled and attempts to provide all information that would be found a corresponding full-dimensional spectrum. It offers following advantages: (1) experimental are used simultaneously, avoiding sensitivity...
An increasing number of extremely premature infants survive the neonatal period and beyond. Little is known about maturation preterm infant's metabolome its relation to development morbidities. Using 1H-NMR, we investigated serum metabolic profile 87 born at a gestational age (GA) <28 weeks [mean GA (SD) 25.4 (1.4) weeks] in samples longitudinally collected from birth term equivalent age. The infant was analyzed GA, postnatal age, nutrition, At day 1, low correlated with high levels...
Optimizing NMR experimental parameters for high-throughput metabolic phenotyping requires careful examination of the total biochemical information obtainable from 1H data, which includes concentration and molecular dynamics information. Here we have applied two different types mathematical transformation (calculation first derivative spectrum Gaussian shaping free-induction decay) to attenuate broad spectral features macromolecules enhance signals small molecules. By application chemometric...
Metabolomics provide an unbiased tool for exploring the modulation of human metabolome in response to food intake. This study applied metabolomics capture postprandial metabolic breakfast meals corresponding vegan (VE), lacto ovo-vegetarian (LOV), and omnivore (OM) diets. In a cross over design 32 healthy volunteers (16 men 16 females) consumed randomized order during three consecutive days. Fasting 3 h serum samples were collected then subjected metabolite profiling using 1H-nuclear...
Objective and reliable methods to measure dietary exposure prove associations causation between diet health are desirable.The aim of this study was investigate if 1H-nuclear magnetic resonance (1H-NMR) analysis serum samples may be used as an objective method discriminate vegan, vegetarian, omnivore diets. Specifically, the identify a metabolite pattern that separated meat-eaters from non-meat-eaters vegans nonvegans.Healthy volunteers (45 men 75 women) complying with habitual vegan (n =...
Biobanks are important infrastructures for life science research. Optimal sample handling regarding e.g. collection and processing of biological samples is highly complex, with many variables that could alter integrity even more complex when considering multiple study centers or using legacy limited documentation on management. Novel means to understand take into account such variability would enable high-quality research archived samples.This investigated whether pre-analytical be predicted...
Metabonomics has been defined as "quantitative measurement of the dynamic multiparametric metabolic response living systems to pathophysiological stimuli or genetic modification" and can provide information on disease processes, drug toxicity, gene function. In this approach many samples biological origin (biofluids such urine plasma) are analyzed using techniques that produce simultaneous detection. A variety analytical profiling tools used routinely, also currently under development,...
It is important to identify patients with Maturity-onset diabetes of the young (MODY) as a molecular diagnosis determines both treatment and prognosis. Genetic testing currently expensive many are therefore not assessed misclassified having either type 1 or 2 diabetes. Biomarkers could facilitate prioritisation for genetic testing. We hypothesised that different underlying aetiologies their have distinct metabolic profiles which may uncover novel biomarkers. The aim this study was perform...
Metabolomics represents a powerful tool for exploring modulation of the human metabolome in response to food intake. However, choice multivariate statistical approach is not always evident, especially complex experimental designs with repeated measurements per individual. Here we have investigated serum metabolic responses two breakfast meals: an egg and ham based cereal using three different approaches on Projections Latent Structures framework. In cross over design, 24 healthy volunteers...
Coupling of evolution periods in NMR experiments is a very promising approach that has recently attracted much attention for its substantial savings measurement time. Another novel concept, which already proved useful many types applications multi-way decomposition. The PRODECOMP tool described here addresses the combination these two modern tools protein NMR. following properties this are and illustrated. Highly similar information to what would be found corresponding full-dimensional...
In the Microvascular Outcomes after Metabolic Surgery randomised clinical trial (MOMS RCT, NCT01821508), combined metabolic surgery (gastric bypass) plus medical therapy (CSM) was superior to alone (MTA) as a means of achieving albuminuria remission at 2-year follow-up in patients with obesity and early diabetic kidney disease (DKD). present study, we assessed urinary 1H-NMR metabolome subgroup from both arms MOMS RCT baseline 6-month follow-up. Whilst CSM MTA reduced excretion sugars,...
The attenuation of diabetic kidney disease (DKD) by metabolic surgery is enhanced pharmacotherapy promoting renal fatty acid oxidation (FAO). Using the Zucker Diabetic Fatty and Sprague Dawley rat models DKD, we conducted studies to determine if these effects could be replicated with a non-invasive bariatric mimetic intervention. Metabolic control injury were compared in rats undergoing dietary restriction plus medical therapy protocol (DMT; fenofibrate, liraglutide, metformin, ramipril,...
The pre-analytical handling of plasma, how it is drawn, processed, and stored, influences its composition. Samples in e.g. biobanks often lack this information consequently important about their quality. Especially metabolite concentrations are affected by making conclusions from metabolomics studies particularly sensitive to misinterpretations. perturbed profile, however, also offers an attractive choice for assessing the history measured data. Here we show that possible using Orthogonal...
Known and consistent bioactivity between samples of insulin is essential to correctly estimate the dose. Insulin concentration not same thing as bioactivity, however, methods determine both are required. Here we show that one dimensional nuclear magnetic resonance (1D NMR), in contrast to, for example, reverse phase high pressure liquid chromatography, can be used well confirm structural integrity required activity. In response report by Carter Heinemann, decided investigate intended public...
Abstract Purpose Choline is an essential nutrient for fetal and infant growth development. Parenteral nutrition used in neonatal care lack free choline but contain small amounts of lipid-bound the form phosphatidylcholine (PC). Here, we examined longitudinal development serum metabolically related compounds betaine methionine extremely preterm infants how concentrations were affected by proportion parenteral fluids received during first 28 postnatal days (PNDs). Methods This prospective...
The metabolome, which is the final down-stream global product of metabolic processes in organisms, not sufficiently described multiple myeloma (MM) patients. aim this study was, therefore, to serum metabolomic profile using proton nuclear magnetic resonance (1H-NMR) spectroscopy, and its relationship clinical characteristics patient outcome. Serum samples, were taken at diagnosis, from 201 MM patients who underwent high-dose melphalan followed by autologous stem cell transplantation as...
Roux-en-Y gastric bypass surgery (RYGB) improves biochemical and histological parameters of diabetic kidney disease (DKD). Targeted adjunct medical therapy may enhance renoprotection following RYGB. The effects RYGB plus fenofibrate, metformin, ramipril, rosuvastatin (RYGB-FMRR) on metabolic control ultrastructural indices glomerular proximal tubular injury were compared in the Zucker Diabetic Sprague Dawley (ZDSD) rat model DKD. Renal cortical transcriptomic (RNA-sequencing) urinary...
This study investigated how postexercise intake of placebo (PLA), protein (PRO), or carbohydrate (CHO) affected fat oxidation (FO) and metabolic parameters during recovery subsequent exercise.In a cross-over design, 12 moderately trained women (VO2max 45 ± 6 ml·min-1·kg-1) performed three days testing. A 23-min control (CON) incremental FO bike test (30-80% VO2max) was followed by 60 min exercise at 75% VO2max. Immediately postexercise, subjects ingested PLA, 20 g PRO, 40 CHO second 2 h...