- biodegradable polymer synthesis and properties
- Algal biology and biofuel production
- Microbial Fuel Cells and Bioremediation
- Photosynthetic Processes and Mechanisms
- Wastewater Treatment and Nitrogen Removal
- bioluminescence and chemiluminescence research
- Microbial Community Ecology and Physiology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Anaerobic Digestion and Biogas Production
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Agriculture, Soil, Plant Science
- Microplastics and Plastic Pollution
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Advanced Polymer Synthesis and Characterization
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine Bivalve and Aquaculture Studies
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Metalloenzymes and iron-sulfur proteins
- Phosphorus and nutrient management
- Microbial metabolism and enzyme function
Utsunomiya University
2013-2024
Tokyo University of Agriculture and Technology
2010-2024
Osaka University
1997-2007
University of Lausanne
2004
Osaka University of Pharmaceutical Sciences
1994-2001
Antibacterial activities against Staphylococcus aureus and Bacillus subtilis were found in an ethanol fraction of tempe, Indonesian fermented soybean produced using Rhizopus oligosporus. The contained free fatty acids, monoglycerides, acid ethyl esters. Among these substances, linoleic α-linolenic exhibited antibacterial S. B. subtilis, whereas 1-monolinolenin 2-monolinolenin activity subtilis. other 1-monoolein, monolinoleins, linoleate, linolenate did not exhibit bactericidal activities....
ABSTRACT A novel whole-cell arsenite biosensor was developed using the photosynthetic bacterium Rhodopseudomonas palustris no. 7 and characterized. sensor plasmid containing operator-promoter region of ars operon arsR gene from Escherichia coli crtI R. introduced into a blue-green mutant with deleted, 711. The changed color in response to arsenite, change obvious naked eye after 24 h without further manipulation. Real-time reverse transcription-PCR showed that mRNA induced 3-fold at 3...
Abstract Growth of and hydrogen production by wild‐type (WT) Rhodovulum sulfidophilum were compared with those one its mutants lacking the poly(3‐hydroxybutyrate) (PHB) biosynthesis ability (PNM2). During phototrophic growth under aerobic conditions fixed illumination, changes in extinction coefficient PHB content WT PNM2 cells revealed interference light penetration PHB. synthesized at an early stage cultivation. degradation after exhaustion acetate during cultivation resulted a decrease...
The role of the Saccharomyces cerevisae peroxisomal acyl-coenzyme A (acyl-CoA) thioesterase (Pte1p) in fatty acid beta-oxidation was studied by analyzing vitro kinetic activity purified protein as well measuring carbon flux through cycle vivo using synthesis polyhydroxyalkanoate (PHA) from polymerization 3-hydroxyacyl-CoAs a marker. amount PHA synthesized degradation 10-cis-heptadecenoic, tridecanoic, undecanoic, or nonanoic acids equivalent slightly reduced pte1Delta strain compared with...
The presence of toxic metals in drinking water has hazardous effects on human health. This study was conducted to develop GFP-based-metal-binding biosensors for on-site assay metal ions. GFP-tagged ArsR and CadC proteins bound a cis element, lost the capability binding it their As- Cd-binding conformational states, respectively. Water samples containing were incubated complex or element which immobilized solid surface. Metal concentrations quantified with fluorescence intensity metal-binding...
ABSTRACT Geraniol is one of the important aromatic ingredients in alcoholic beverages. Bioconversions geraniol to other terpenoids and genes involved oxidation were investigated. Geranic acid citronellic detected yeast culture, where or nerol was added. Addition citral, a mixture geranial neral, resulted production geranic acid, whereas addition citral citronellal suggesting that might be produced through conversion followed by citronellal. Consumption citronellol affected adh1Δ, adh3Δ,...
Hydrogen peroxide was cytotoxic to the small intenstine epithelial cell line, IEC-6, as judged from an MTT assay and release of lactate dehydrogenase. The glutathione S-transferase thioredoxin reductase activities SH content decreased dose-dependently with H2O2, but activity increased at low H2O2 concentrations. In addition, increase in time-dependent during initial stages oxidative stress. A reverse transcription-polymerase chain reaction (RT-PCR) amplification also showed that mRNA IEC-6...