Cardiogenesis is inhibited by canonical Wnt/beta-catenin signalling and stimulated non-canonical Wnt11/JNK signalling, but how these two pathways crosstalk currently unknown. Here, we show that restricts cardiogenesis via inhibition of GATA gene expression, as experimentally reinstating function overrides beta-catenin-mediated restores cardiogenesis. Furthermore, transcription factors in turn directly regulate Wnt11 required to a significant degree for mediating the cardiogenesis-promoting...

The individual contributions of the three vertebrate GATA factors to endoderm formation have been unclear. Here we detail early expression GATA4, 5 and 6 in presumptive Xenopus embryos their induction endodermal markers ectoderm. Induction HNF3beta by all was abolished when protein synthesis inhibited, showing that these inductions are indirect. In contrast, whereas Sox17alpha HNF1beta GATA4 substantially reduced GATA6 minimally affected, suggesting is a direct activator genes. induced same...

Abstract A functioning heart muscle is required continuously throughout life. During embryonic development the tissue differentiates from mesoderm that has heart‐forming potential. Heart‐forming potential in regulated by pro‐cardiogenic transcription factors, such as members of GATA and NK‐2 factor families. Subsequent differentiation involves expression cytoskeletal proteins, including myosins troponins. Different Wnt signalling pathways have various functions development. So‐called...

Secreted molecules called morphogens govern tissue patterning in a concentration-dependent manner. However, it is still unclear how reproducible can be achieved with diffusing molecules, especially when that concerns differentiation of thin tissues. Wnt morphogen organizes cardiac development. Wnt6 patterns cardiogenic mesoderm to induce tissue, the pericardium, Xenopus. In this study, we revealed receptor, frizzled-7, expressed Wnt-dependent With combination experiments and mathematical...

Abstract GATA factors and Wnt signals are key regulators of vertebrate cardiogenesis, but specific roles for individual how they interact with signaling remain unknown. We use loss function overexpression approaches to elucidate these molecules regulate early cardiogenesis in Xenopus . In order minimize indirect effects due abnormal embryogenesis, we pluripotent embryonic tissues as cardiogenic assays. confirm central GATA4, 5, 6 also discover different requirements. show that GATA4 or both...

The transcription factors GATA4, GATA5 and GATA6 are important regulators of heart muscle differentiation (cardiomyogenesis), which function in a partially redundant manner. We identified genes specifically regulated by individual cardiogenic GATA genome-wide transcriptomics analysis. gata4 particularly interesting because GATA4 is able to induce beating cardiomyocytes Xenopus mammalian systems. Among the gata4-regulated transcripts we two SoxF family members, sox7 sox18. Experimental...

Although Wnt/β-catenin signaling is generally conserved and well understood, the regulatory mechanisms controlling context-specific direct Wnt target gene expression in development disease are still unclear. The onset of zygotic transcription early embryogenesis represents an ideal, accessible experimental system to investigate regulation. We combine transcriptomics using RNA-seq with genome-wide β-catenin association ChIP-seq identify stage-specific genes. propose coherent feedforward...

The transcription factors GATA4, GATA5 and GATA6 play important roles in heart muscle differentiation. data presented this article are related to the research entitled "Genome-wide transcriptomics analysis identifies sox7 sox18 as specifically regulated by gata4 cardiomyogenesis" (Afouda et al., 2017) [1]. present study genes these individual cardiogenic GATA using genome-wide analysis. We have that each of them, well those either them. gene ontology terms (GO) associated with differentially...

We agree that the discrepancy between our results on Xenopus cardiac MHCα expression reported in Afouda et al. (Afouda al., 2008) and those of Samuel Latinkic based their experiments (Samuel Latinkic, 2010) might indeed be due to technical details. Our analysis is full-length sequence [accession no. AY913767 (Garriock 2005)], using PCR primers developed by Professor Asashima's research group (see Ariizumi 2003): forward, 5′-GCCAACTCAAACCTCTCCAAGTTCCG-3′ reverse,...

Inhibition of Wnt/β‐catenin signalling is required in vertebrate embryos for initiation heart development. The identity the endogenous Wnt ligand that needs to be inhibited has so far remained unclear. We identified Wnt6 expressed close forming tissue Xenopus. Endogenous expression during organogenesis prevent differentiation an abnormally large muscle. Even at these remarkably late stages, Wnt6/β‐catenin regulates pro‐cardiogenic transcription factor network rather than muscle directly. Our...

Abstract Secreted molecules called morphogens govern tissue patterning in a concentration-dependent manner. However, it is still unclear how reproducible can be achieved with diffusing molecules, especially when differentiation of thin region. Wnt morphogen that organizes cardiac development; Wnt6 patterns the cardiogenic mesoderm to induce pericardium Xenopus . It is, however, pattern such tissue. In this study, we reveal receptor, frizzled7 , expressed Wnt-dependent manner prospective...