A5103259966- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- Marine Toxins and Detection Methods
- Neuroendocrine regulation and behavior
- Insect and Pesticide Research
- S100 Proteins and Annexins
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Molecular Sensors and Ion Detection
- Mitochondrial Function and Pathology
- Calcium signaling and nucleotide metabolism
- Lipid Membrane Structure and Behavior
- Research on Leishmaniasis Studies
- Cancer-related Molecular Pathways
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Neurotransmitter Receptor Influence on Behavior
- Trypanosoma species research and implications
Centre National de la Recherche Scientifique
1992-2022
Université Paris-Saclay
2017-2022
Frédéric Joliot Institute for Life Sciences
2018-2022
Technologies pour la Santé
2018-2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2020
CEA Paris-Saclay
2015-2020
Institut de Biologie et Technologies
2015-2019
Direction de la Recherche Fondamentale
2019
Institut des Neurosciences Paris-Saclay
2016-2019
Université Paris-Sud
2011-2018
Several studies have suggested that the V0 domain of vacuolar-type H+-adenosine triphosphatase (V-ATPase) is directly implicated in secretory vesicle exocytosis through a role membrane fusion. We report this paper there was rapid decrease neurotransmitter release after acute photoinactivation a1-I subunit neuronal pairs. Likewise, inactivation chromaffin cells resulted decreased frequency and prolonged kinetics amperometric spikes induced by depolarization, with shortening fusion pore open...
Abstract: The effects of the nicotinic agonist (+)‐anatoxin‐a have been examined in four different preparations, representing at least two classes neuronal receptors. (+)‐Anatoxin‐a was most potent (EC 50 = 48 n M ) stimulating 86 Rb + influx into M10 cells, which express receptor subtype comprising α4 and β2 subunits. A presynaptic mediating acetylcholine release from hippocampal synaptosomes similarly sensitive to 140 ). α‐Bungarotoxin‐sensitive receptors, studied using patch‐clamp...
Abstract Gymnodimines (GYMs) are phycotoxins exhibiting unusual structural features including a spirocyclic imine ring system and trisubstituted tetrahydrofuran embedded within 16‐membered macrocycle. The toxic potential the mechanism of action GYM‐A, highly purified from contaminated clams, have been assessed. GYM‐A in isolated mouse phrenic hemidiaphragm preparations produced concentration‐ time‐dependent block twitch responses evoked by nerve stimulation, without affecting directly...
In Trypanosoma brucei, the actin gene is present in a cluster of two, three, or four tandemly linked copies, depending on strain. Each seems to exist two allelic versions, as suggested by polymorphism both number and restriction fragment length DNA from cloned trypanosomes. The amplification copy probably occurs through unequal sister chromatid exchange. chromosomes harboring genes belong large size class. coding sequence was 1,128 nucleotides long showed 60 70% homology other eucaryotic...
Fundamental brain functions depend on a balance between excitation (E) and inhibition (I) that is highly adjusted to 20–80% set point in layer 5 pyramidal neurons (L5PNs) of rat visual cortex. Dysregulations both the E–I serotonergic system neocortical networks lead serious neuronal diseases including depression, schizophrenia, epilepsy. However, no link activation 5-hydroxytryptamine receptors (5-HTRs) cortical has yet been reported. Here we used combination patch-clamp recordings composite...
Abstract Diabetes is associated with a hypercoagulable state that contributes to macrovascular complications, including cardiovascular events. The glycation reaction, consequence of chronic hyperglycemia, has also been implicated in the pathogenesis diabetic complications. Glycated proteins have receptors on monocytes and generate reactive oxygen species can regulate expression number genes. As abnormal monocyte tissue factor (TF), main initiator coagulation cascade, responsible for...
The potencies and efficacies of seven agonists at chick α7 nicotinic receptors expressed in Xenopus oocytes were determined by whole cell recording. (+)‐Anatoxin‐a was the most potent agonist (EC 50 = 0.58 μM) acetylcholine least 320 μM). rank order was: (+)‐anatoxin‐a ⪢ cytisine > (−)‐nicotine (+)‐nicotine DMPP 1‐acetyl‐4‐methylpiperazine methiodide acetylcholine. evoked only very small currents: comparison maximally effective concentrations showed that one‐fifth as efficacious other...
This study aims to clarify how endogenous release of cortical acetylcholine (ACh) modulates the balance between excitation and inhibition evoked in visual cortex. We show that electrical stimulation layer 1 produced a significant ACh measured intracortically by chemoluminescence composite synaptic response recorded intracellularly 5 pyramidal neurons rat The pharmacological specificity neuromodulation was determined from continuous whole-cell voltage clamp measurement stimulation-locked...
N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity. Their activation requires the binding of both glutamate and d-serine or glycine as co-agonist. The prevalence either co-agonist on NMDA-receptor function differs between brain regions remains undetermined visual cortex (VC) at critical period postnatal development. Here, we therefore investigated regulatory that and/or may exert NMDARs plasticity rat VC layer 5 pyramidal neurons young rats. Using selective...
Abstract Homeostatic regulation in the brain is thought to be achieved through a control of synaptic strength by close interactions between excitation and inhibition cortical circuits. We recorded layer 5 pyramidal neuron rat cortex composite response an electrical stimulation various layers (2–3, 4 or 6). Decomposition global conductance change its excitatory inhibitory components permits direct measurement excitation–inhibition (E‐I) balance. Whatever stimulated was, afferent inputs led...
Mutations in the p21-activated kinase 3 gene (pak3) are responsible for nonsyndromic forms of mental retardation. Expression mutated PAK3 proteins hippocampal neurons induces abnormal dendritic spine morphology and long term potentiation anomalies, whereas pak3 invalidation leads to cognitive impairments. How regulates synaptic plasticity is still largely unknown. To better understand how affects neuronal plasticity, we focused on its interaction with Nck adaptors that play a crucial role...
Spirolides are a large family of lipophilic marine toxins produced by dinoflagellates that have been detected in contaminated shellfish. Among them, 13,19-didesmethyl and 13-desmethyl spirolide C phycotoxins widely distributed their mode action needs to be clearly defined. In order further characterize the pharmacological profiles these on various nicotinic acetylcholine receptor (nAChR) subtypes examine whether they act muscarinic receptors (mAChRs), functional electrophysiological studies...
In the cortex, homeostatic plasticity appears to be a key process for maintaining neuronal network activity in functional range. This phenomenon depends on close interactions between excitatory and inhibitory circuits. We previously showed that application of high frequency stimulation (HFS) protocol layer 2/3 induces parallel potentiation inputs 5 pyramidal neurons, leading an unchanged excitation/inhibition (E/I) balance. These coordinated long-term potentiations excitation inhibition...
p21-activated kinase 1 (PAK1) and PAK3 belong to group I of the PAK family control cell movement division. They also regulate dendritic spine formation maturation in brain, play a role synaptic transmission plasticity. PAK3, particular, is known for its implication X-linked intellectual disability. The pak3 gene expressed neurons as GTPase-regulated PAK3a protein three splice variants which display constitutive activity. PAK1 regulation based on homodimerization, forming an inactive complex....
Medical means to save the life of human patients affected by drug abuse, envenomation or critical poisoning are currently limited. While compounds at risks most often well identified, particularly for bioterrorism, chemical intervention counteract toxic effects ingested/injected compound(s) is restricted use antibodies. Herein, we illustrate that DNA aptamers, targeted block pharmacophore a poisonous compound, represent fast-acting and reliable method neutralization in vivo possesses...
Vacuolar proton ATPase accumulates protons inside various intracellular organelles such as synaptic vesicles; its membrane domain V0 could also be involved in fusion. These different functions require vacuolar ATPases possessing subunit a isoforms. In vertebrates, four genes encode isoforms a1–a4, and a1 variants are generated by alternative splicing. We identified novel splice variant a1-IV showed that the two containing exon C specifically expressed neurons. Single neurons coexpress a2,...
Autism spectrum disorder (ASD) is characterized by early onset of behavioral and cognitive alterations. Low plasma levels oxytocin (OT) have also been found in ASD patients; recently, a critical role for the enzyme CD38 regulation OT release was demonstrated. important regulating several Ca2+-dependent pathways, but beyond its secretion, it not known whether deficit expression leads to functional modifications prefrontal cortex (PFC), structure involved social behavior. Here, we report that...
We describe the isolation of a molluscan (Lymnaea stagnalis) full-length complementary DNA that encodes mature polypeptide (which we have named Lym-eGluR2) with predicted molecular weight 105 kDa exhibits 44-48% identity to mammalian kainate-selective glutamate receptor GluR5, GluR6, and GluR7 subunits. Injection in vitro-transcribed RNA from this clone into Xenopus laevis oocytes results robust expression homo-oligomeric cation channels can be gated by L-glutamate (EC50 = 1.2 +/- 0.3...