A5024447290- Biomedical and Engineering Education
- RNA Research and Splicing
- Endometriosis Research and Treatment
- Hearing, Cochlea, Tinnitus, Genetics
- Uterine Myomas and Treatments
- Intestinal and Peritoneal Adhesions
- Insect and Arachnid Ecology and Behavior
- Plant Reproductive Biology
- Cancer, Hypoxia, and Metabolism
- Lymphoma Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Fibroblast Growth Factor Research
- Plant and animal studies
- Bone Metabolism and Diseases
- RNA modifications and cancer
- Nicotinic Acetylcholine Receptors Study
- RNA regulation and disease
- Biosimilars and Bioanalytical Methods
- Nuclear Receptors and Signaling
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
- Crime Patterns and Interventions
- Ear Surgery and Otitis Media
Lahey Medical Center
2020
Christiana Care Health System
2018
European Bioinformatics Institute
2011-2015
Wellcome Trust
2011-2013
Pfizer (United Kingdom)
2002-2009
Long Island Jewish Medical Center
2007
University of Sheffield
2002
MRC Laboratory of Molecular Biology
1997
University of Bath
1993
University of Massachusetts Amherst
1978
Calcium ions are the principal second messenger in control of gene expression by electrical activation neurons. However, full complexity calcium-signaling pathways leading to transcriptional and cellular machinery involved not known. Using c-<i>fos</i> as a model system, we show here that activity its complex promoter is controlled three independently operating signaling mechanisms their functional significance cell type-dependent. The serum response element (SRE), which composed ternary...
Abstract Background Wound healing is a complex process requiring the collaborative efforts of different tissues and cell lineages, involving coordinated interplay several phases proliferation, migration, matrix synthesis contraction. Tissue damage also triggers robust influx inflammatory leukocytes to wound site that play key roles in clearing invading microbes but release signals may be detrimental repair lead fibrosis. Results To better define cellular events pivotal for tissue yet...
The potencies and efficacies of seven agonists at chick α7 nicotinic receptors expressed in Xenopus oocytes were determined by whole cell recording. (+)‐Anatoxin‐a was the most potent agonist (EC 50 = 0.58 μM) acetylcholine least 320 μM). rank order was: (+)‐anatoxin‐a ⪢ cytisine > (−)‐nicotine (+)‐nicotine DMPP 1‐acetyl‐4‐methylpiperazine methiodide acetylcholine. evoked only very small currents: comparison maximally effective concentrations showed that one‐fifth as efficacious other...
We have used Affymetrix high-density gene arrays to generate a temporal profile of expression during differentiation UB/OC-1, conditionally immortal cell line derived from the mouse cochlea. Gene was assessed daily for 14 days under differentiating conditions. The experiment replicated in two separate populations cells. Profiles selected genes were correlated with those obtained by RT-PCR, TaqMan analysis, immunoblotting, and immunofluorescence. results suggest that UB/OC-1 is population...
We have studied the function of zinc finger transcription factor gata3 in auditory system development by analysing temporal profiles gene expression during differentiation conditionally immortal cell lines derived to model specific types and developmental stages. tested applied a novel probabilistic method called gamma Model for Oligonucleotide Signals analyse hybridization signals from Affymetrix oligonucleotide arrays. Expression levels estimated this correlated closely (p<0.0001) across...
Abstract Conditionally immortal cell lines were established from the ventral otocyst of Immortomouse at embryonic day 10.5 and selected to represent precursors auditory sensory neural epithelial cells. Selection was based upon dissection, tissue‐specific markers, expression transcription factor GATA3. Two expressed GATA3 but possessed intrinsically different genetic programs under differentiating conditions. US/VOT‐E36 represented progenitors with potential differentiate into nonsensory...
Many flowering plants are unable to set seeds with their own pollen because a system known as gametophytic self-incompatibility is operating. The basis of this single multiallelic locus S, and if the S allele carried by grain matches one two alleles in style, it certain do upon self-pollination, then tube growth inhibited. Should self-pollen grains carry mutated allele, however, would not match either those style therefore, be Gametophytic thus provides mechanism for discriminating between...
7092 Background: We are conducting a pilot, exploratory study of the potential value alemtuzumab(alem) in maintenance therapy previously treated chronic lymphocytic leukemia (CLL) patients(pts) after they have achieved stable disease or partial remission with chemo chemo-immunotherapy. present results serially monitored CD19+ (B)lymphocytes and CD4+ (T) lymphocytes on eight evaluable patients. Methods: 30mg doses alem were administered SC to all patients at following schedule: wkly for 8 (8...