A5014359310- Nitrogen and Sulfur Effects on Brassica
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Renal and related cancers
- CRISPR and Genetic Engineering
- Genetics and Plant Breeding
- Antibiotics Pharmacokinetics and Efficacy
- Lipid metabolism and biosynthesis
- Agricultural Science and Fertilization
- Advances in Cucurbitaceae Research
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Natural Antidiabetic Agents Studies
- Sperm and Testicular Function
- Antibiotic Resistance in Bacteria
- Reproductive Physiology in Livestock
- Plant Pathogens and Resistance
- Agricultural Practices and Plant Genetics
- Tissue Engineering and Regenerative Medicine
- Potato Plant Research
- Milk Quality and Mastitis in Dairy Cows
- Connexins and lens biology
- Plant Disease Resistance and Genetics
Govind Ballabh Pant University of Agriculture and Technology
2002-2024
Botanical Survey of India
2014
University of Maryland, College Park
2004-2009
Georgetown University
2008-2009
Georgetown University Medical Center
2008-2009
North Dakota State University
2004-2008
Technical University of Munich
2006
University of Wisconsin–Madison
2006
Recent reports have demonstrated that adult tissue cells can be induced to pluripotency, the iPS cells, mostly with addition of genes delivered using viruses. Also, several publications both in mouse and human spermatogonial stem (SSCs) from testes convert back embryonic (ES)-like without genes. Furthermore, these pluripotent ES-like differentiate into all three germ layers organ lineages. Thus, SSCs great potential for cell-based, autologous regeneration therapy various diseases. We...
Transcription factors, POU5F1/OCT4 and NANOG, whose expression is restricted to the inner cell mass (ICM) in mouse human blastocysts, are used characterize undifferentiated embryonic stem cells (ESC) vitro. However, POU5F1 may not be a useful marker domestic animals due its both ICM trophectoderm (TE), while NANOG mRNA protein have only been described fully mice. In an effort identify ESC markers for animals, patterns of POU5F1, surface (SSEA1, SSEA4, TRA-1-60, TRA-1-81) were examined...
Abstract Embryonic stem (ES) cells can differentiate into all three embryonic germ layers but rarely trophectoderm (TE) lineages that contribute to the placenta, although TE differentiation be initiated by genetic manipulation of key genes involved in development. We demonstrate Wnt signaling initiate lineage triggering an appropriate cue, caudal-related homeobox 2 (Cdx2). Overexpression and RNA interference knockdown studies indicate Cdx2 induction response Wnt3a is mediated lymphoid...
Recent findings identifying the transcription factors involved in regulation of pluripotency and self-renewal embryonic stem cells (ESC) may provide keys that enable derivation ESC domestic species. In this study we monitored expression pluripotency-related genes bovine inner cell mass (ICM) explants during critical first steps establishment primary cultures. The NANOG POU5F1 transcripts proteins intact, vitro produced (IVP) blastocysts was confirmed by quantitative RT-PCR fluorescent...
Enrofloxacin and Ciprofloxacin antibiotics are widely used in chicken production for prophylaxis therapeutics purposes. Existence of these antibiotic residues meat can pose hazards to human health. The present study was aimed assess the residue level meat. Chicken samples (including muscle, liver, kidney fat) from poultry farms retail market were collected. High Performance Liquid Chromatography (HPLC) screening enrofloxacin ciprofloxacin samples. analysis revealed that 43.58% positive...
To evaluate the effects of FSH, LH and/or cAMP on expression connexin 43 (Cx43) in ovine cumulus-oocyte complex (COC) and gap junctional intercellular communication (GJIC) cumulus cells, two experiments were carried out. In experiment 1, Cx43 was immunodetected COC, before or after maturation, obtained from non-treated FSH-treated ewes. The COC greater ( P < 0.01) day 16 than 15 estrous cycle. vivo FSH treatment decreased 0.02) but not 2, intact COCs isolated cells small large follicles...
The pharmacokinetics of Florfenicol was studied in broilers following its oral administration @30 mg.kg-1b.w. Blood samples were taken at various time intervals and the concentration plasma determined using high performance liquid chromatography (HPLC).The peak 1 hour 3.314µg.ml-1.The calculated Cmax Tmax 3.130µg. mL-1 0.963 h, respectively. distribution half-life(t1/2α) 0.625 h elimination half-life (t1/2β) 6.421 h. mean area under curve (AUC) as 13.352 µg.mL-1. Volume (V/F) 7.934 L.kg-1...
A review of Indian Riccia’s has been made and 36 valid species are accepted. 12 viz., R. attenuata, ciliata, crispatula, crozalsii, curtisii, glauca, haskarliana, hirta, mangalorica, perssonii, stricta warnstorfii rare found in restricted pockets the country. Except Riccia abuensis fluitans (aquatic species) all terrestrial. poihaiana A.E.D.Daniels & P.Daniel is proposed to place under synonymy billardieri Mont. Nees. The occurrence squamata, duplex dictyospora recorded bryoflora treated...
Background: Trianthema portulacastrum Linn. is an exotic plant growing as weed in Africa, Southeast Asia and tropical America. Chemical fingerprinting under the present study was undertaken to characterize polyphenolic compounds antioxidant activities plant. Methods: Several vitro assays were performed evaluate aqueous, alcoholic hydro-alcoholic extracts. Characterization of done by HPLC GCMS analyses. Result: Total phenols, flavonoids total activity assessed DPPH, ABTS capacity found be...
Genetic modification of embryonic stem (ES) cells derived from domestic species could be exploited to produce transgenic animals; however, fully validated ES have not been obtained in species. Recent findings regarding key transcription factors and regulation pluripotency self-renewal murine may provide keys enable the derivation The aim this study was identify monitor expression candidate genes, which are known involved maintenance mouse human cells, during critical first steps...
The objective of this study was to determine the expression patterns pluripotency-determining factors, Oct-4 and Nanog, in pre-implantation goat embryos. POU octamer-binding domain transcription factor homeobox Nanog have been shown play key roles maintenance pluripotency inner cell mass (ICM) mouse embryos embryonic stem cells. As protein has observed human, monkey, bovine, porcine embryos, its role development differentiation may be conserved across these species. mRNA for not reported...