A5043454566- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Kruppel-like factors research
- Congenital heart defects research
- Reproductive Biology and Fertility
- Cancer-related gene regulation
- melanin and skin pigmentation
- Regulation of Appetite and Obesity
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Connexins and lens biology
- Traumatic Brain Injury and Neurovascular Disturbances
- Integrated Circuits and Semiconductor Failure Analysis
National Institute of Dental and Craniofacial Research
2023
National Institutes of Health
2023
University of Maryland, College Park
2006-2018
VA Western New York Healthcare System
2003-2004
University at Buffalo, State University of New York
2003-2004
Oregon National Primate Research Center
2001
Nontypeable Haemophilus influenzae colonizes the respiratory tract of adults with chronic obstructive pulmonary disease (COPD) and causes intermittent exacerbations. Isolates H. collected monthly in a prospective study were subjected to molecular typing. During 7-year spanning 345 patient-months observation, 122 episodes negative cultures lasting 1 month or more, that preceded followed by isolation an apparently identical strain influenzae, found. Seventeen such cultures, 6 months more 203...
Atomic force microscopy (AFM) has emerged as a promising tool to characterize the mechanical properties of biological materials and cells. In our studies, undifferentiated early differentiating mouse embryonic stem cells (mESCs) were assessed individually using an AFM system determine if we could detect changes in their by surface probing. Probes with pyramidal spherical tips assessed, different analytical models for evaluating data. The combination probing tip analysis Hertz model provided...
Transcription factors, POU5F1/OCT4 and NANOG, whose expression is restricted to the inner cell mass (ICM) in mouse human blastocysts, are used characterize undifferentiated embryonic stem cells (ESC) vitro. However, POU5F1 may not be a useful marker domestic animals due its both ICM trophectoderm (TE), while NANOG mRNA protein have only been described fully mice. In an effort identify ESC markers for animals, patterns of POU5F1, surface (SSEA1, SSEA4, TRA-1-60, TRA-1-81) were examined...
The epithelial-to-mesenchymal transition (EMT) is a highly coordinated process underlying both development and disease. Premigratory neural crest cells undergo EMT, migrate away from the tube, differentiate into diverse cell types during vertebrate embryogenesis. Adherens junction disassembly within premigratory one component of EMT and, in chick cranial cells, involves cadherin-6B (Cad6B) down-regulation. Whereas Cad6B transcription repressed by Snail2, rapid loss protein suggestive...
Abstract The ability of the pluripotent epiblast to contribute progeny all three germ layers is thought be lost after gastrulation. later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential generate mesodermal- endodermal-like derivatives obtained. Here, we monitor transcriptional changes gastrulation neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. We show...
Abstract Embryonic stem (ES) cells can differentiate into all three embryonic germ layers but rarely trophectoderm (TE) lineages that contribute to the placenta, although TE differentiation be initiated by genetic manipulation of key genes involved in development. We demonstrate Wnt signaling initiate lineage triggering an appropriate cue, caudal-related homeobox 2 (Cdx2). Overexpression and RNA interference knockdown studies indicate Cdx2 induction response Wnt3a is mediated lymphoid...
The bovine trophectoderm (TE) undergoes a dramatic morphogenetic transition prior to uterine endometrial attachment. Many studies have documented trophoblast-specific gene expression profiles at various pre-attachment stages, yet genetic interactions within the transitioning TE regulatory network are not well characterized. During embryogenesis, transcription factors OCT4 and CDX2 co-expressed during early trophoblast elongation. In this study, trophectoderm-derived CT-1 cell line was...
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, permit their EMT and migration. Serial processing of Cad6B by a disintegrin metalloproteinase (ADAM) proteins γ-secretase generates intracellular C-terminal fragments (CTF2s) that could acquire additional functions. Here we report CTF2...
In rodents, stimulation of melanocortin-3 and -4 receptor subtypes (MC3-R MC4-R) causes a reduction in food intake, whereas antagonism MC3-R MC4-R increases intake. This report describes the effects stable αMSH analog, NDP-MSH ([Nle4, d-Phe7]αMSH), endogenous antagonist, agouti-related protein, on feeding behavior adult male rhesus macaques. Infusion into lateral cerebral ventricle dose dependently suppressed intake normally scheduled meal without affecting nonfeeding behaviors. Conversely,...
An adult with chronic obstructive pulmonary disease was monitored prospectively for 2 years. Nontypeable Haemophilus influenzae isolated from sputum cultures at 22 of 23 monthly clinic visits. Analysis the isolates, by pulsed-field gel electrophoresis (PFGE), revealed that patient colonized 3 different strains during 2-year period. The gene encoding outer-membrane protein (OMP) P2, ompP2, amplified samples and selected obtained this patient. ompP2 sequences, in combination PFGE patterns,...
The ability of the pluripotent epiblast to contribute progeny all three germ layers is thought be lost after gastrulation. later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential generate mesodermal- endodermal-like cells obtained. We monitored transcriptional changes gastrulation neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. Unexpectedly, we find...
Cloned bovine embryos have been shown to display deviations in gene expression of trophectodermal (TE) lineage specific transcription factors, including CDX2, ERR2, EOMES, MASH2 and HAND1. Misregulation these genes the early TE has implicated play a causal role abnormal placental formation fetal loss. Therefore, it is particularly important gain better understanding mechanisms controlling embryo development during transitional elongation stage. In preliminary studies, we that CT-1 cell line...
Cloned bovine embryos have been shown to display deviations in gene expression of trophectodermal (TE) lineage-specific transcription factors, including Cdx2, Errβ, Eomes, Mash2, and Hand1. Misregulation these genes the early TE has implicated play a causal role abnormal placental formation fetal loss. Because suboptimal vitro culture conditions fail support development beyond hatching, very little is known about regulation peri-attachment embryo development. Trophectodermal-derived cell...
Strategies utilized in deriving embryonic stem (ES) cells from mouse and primate embryos have not been sufficient for maintaining self renewal inhibiting spontaneous differentiation ungulate embryo explant cultures, implying that ESC may require a unique signature of signal transduction pathways, transcription factor hierarchies, chromatin modifications. To determine how key factors OCT4 (POU5F1) SOX2 induce changes developmental potential within differentiated bovine cells, we constructed...