A5045358263- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Connexins and lens biology
- Cancer-related gene regulation
- Axon Guidance and Neuronal Signaling
- Congenital heart defects research
- Hereditary Neurological Disorders
- Barrier Structure and Function Studies
- Neuropeptides and Animal Physiology
- Neurological diseases and metabolism
- Craniofacial Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Signaling Pathways in Disease
- Kruppel-like factors research
- Hippo pathway signaling and YAP/TAZ
- Calpain Protease Function and Regulation
- Neonatal Respiratory Health Research
- Hedgehog Signaling Pathway Studies
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- dental development and anomalies
- Hearing, Cochlea, Tinnitus, Genetics
- Animal Genetics and Reproduction
- Medical and Biological Sciences
- S100 Proteins and Annexins
University of Maryland, College Park
2015-2024
Institut de la Vision
2013
California Institute of Technology
2004-2007
Stanford University
1998
University of Leeds
1998
Molecular Oncology (United States)
1998
Princeton University
1997-1998
McDaniel College
1995-1996
National Institutes of Health
1995
Wnt family members are critical to many developmental processes, and components of the signaling pathway have been linked tumorigenesis in familial sporadic colon carcinomas. Here we report identification two genes, WISP-1 WISP-2, that up-regulated mouse mammary epithelial cell line C57MG transformed by Wnt-1, but not Wnt-4. Together with a third related gene, WISP-3, these proteins define subfamily connective tissue growth factor family. Two distinct systems demonstrated WISP induction be...
The neural crest, a transient population of migratory cells, forms the craniofacial skeleton and peripheral nervous system, among other derivatives in vertebrate embryos. transcriptional repressor Snail2 is thought to be crucial for epithelial-to-mesenchymal transition (EMT) that promotes crest delamination from tube; however, little known about its downstream targets. To this end, we depleted avian premigratory using morpholino antisense oligonucleotides examined effects on potential...
Precaution can be consistent with support of science
The epithelial-to-mesenchymal transition (EMT) is a highly coordinated process underlying both development and disease. Premigratory neural crest cells undergo EMT, migrate away from the tube, differentiate into diverse cell types during vertebrate embryogenesis. Adherens junction disassembly within premigratory one component of EMT and, in chick cranial cells, involves cadherin-6B (Cad6B) down-regulation. Whereas Cad6B transcription repressed by Snail2, rapid loss protein suggestive...
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, permit their EMT and migration. Serial processing of Cad6B by a disintegrin metalloproteinase (ADAM) proteins γ-secretase generates intracellular C-terminal fragments (CTF2s) that could acquire additional functions. Here we report CTF2...
ABSTRACT During embryonic development, diverse cell types coordinate to form functionally complex tissues. Exemplifying this process, the trigeminal ganglion emerges from condensation of two distinct precursor populations, cranial placodes and neural crest, with neuronal differentiation former preceding latter. While its dual cellular origin has been understood for decades, molecules orchestrating formation remain relatively obscure. Initial assembly is mediated by adhesion molecules,...
Abstract Computer modeling studies have been carried out on three nonnucleoside inhibitors complexed with human immunodeficiency virus type 1 (HIV‐1) reverse transcriptase (RT), using crystal coordinate data from a subset of the protein surrounding binding pocket region. Results minimizations solvated complexes 2‐cyclopropyl‐4‐methyl‐5,11‐dihydro‐5 H ‐dipyrido[3,2‐ b :2′,3′‐ e ][1,4]diazepin‐6‐one (nevirapine), α‐anilino‐2, 6‐dibromophenylacetamide (α‐APA), and...
The Wnt signaling pathway is important in the formation of neural crest cells many vertebrates, but downstream targets induction by are largely unknown. Here, we examined quantitative changes gene expression regulated Wnt-mediated using PCR (QPCR). Induction was recapitulated vitro adding soluble to intermediate plate tissue cultured collagen, and induced versus control were assayed gene-specific primers at times corresponding premigratory (18 24 h) or early (36 stages migration. results...
Neural crest cells give rise to a diverse range of structures during vertebrate development. These initially exist in the dorsal neuroepithelium and subsequently acquire capacity migrate. Although studies have documented importance adherens junctions regulating neural cell migration, little attention has been paid tight this process. We now identify junction protein cingulin as key regulator migration. Cingulin knock-down increases migratory domain, which is correlated with disruption tube...
Gap junctions are intercellular channels between cells that facilitate cell-cell communication. Connexin 43 (Cx43; also known as GJA1), the predominant gap junction protein in vertebrates, is expressed premigratory cranial neural crest and maintained throughout cell epithelial-to-mesenchymal transition (EMT), but its function these unknown. To this end, we used a combination of
Familial dysautonomia (FD) is a sensory and autonomic neuropathy caused by mutations in elongator complex protein 1 ( ELP1 ). FD patients have small trigeminal nerves impaired facial pain temperature perception. These signals are relayed nociceptive neurons the ganglion, structure that composed of both neural crest- placode-derived cells. Mice lacking Elp1 crest derivatives (‘ CKO’) born with ganglia, suggesting important for ganglion development, yet function this context unknown. We...
The epithelial-to-mesenchymal transition (EMT) is critical for the formation of migratory neural crest cells during development and co-opted in human diseases such as cancer metastasis. Chick premigratory cranial lose intercellular contacts, mediated part by Cadherin-6B (Cad6B), migrate extensively, later form a variety adult derivatives. Importantly, modulation Cad6B proper cell EMT. Although possesses long half-life, it rapidly lost from membranes, suggesting existence post-translational...