Cot-based sequence discovery represents a powerful means by which both low-copy and repetitive sequences can be selectively efficiently fractionated, cloned, characterized. Based upon the results of Cot analysis, hydroxyapatite chromatography was used to fractionate sorghum ( Sorghum bicolor ) genomic DNA into highly (HR), moderately (MR), single/low-copy (SL) components that were consequently cloned produce HRCot, MRCot, SLCot libraries. Filter hybridization (blotting) analysis show HRCot...

We challenged chickens, turkeys, ducks, quail, and geese with severe acute respiratory syndrome coronavirus 2 or Middle East coronavirus. observed no disease detected virus replication serum antibodies. concluded that poultry are unlikely to serve a role in maintenance of either virus.

Highly pathogenic (HP) avian influenza viruses (AIVs) of the clade 2.3.4.4 goose/Guangdong/1996 H5 lineage continue to be a problem in poultry and wild birds much world. The recent incursion H5N1 2.3.4.4b HP AIV from this into North America has resulted widespread outbreaks consistent detections virus across diverse families occasionally mammals. To characterize pathobiology mallards (Anas platyrhynchos), which are primary reservoir AIV, challenge study was conducted with 2-week-old birds....

ABSTRACT Many hospitality curricula incorporate some form of industry-based experiential learning to complement the classroom environment. This study analyzed management student perceptions both inside environment and in their assignments outside classroom. A review literature found documented benefits learning. confirmed many these previously identified new outcomes or for students who participate learning, such as an increased understanding how organizations function, ability view career...

Abstract Healthcare fraud and overutilization pose significant challenges in the United States, leading to substantial financial losses compromised patient care. Medicare, a vital federal healthcare program, is particularly susceptible such abuses. With over 63 million Americans enrolled Medicare growing expenses, need for effective detection paramount. Traditional methods relying on manual audits have proven insufficient, allowing portion of fraudulent activity go undetected. Machine...

Highly pathogenic avian influenza virus (HPAIV) has caused widespread outbreaks in poultry the Americas. Because of duration and extent these outbreaks, vaccine use may be an additional tool to limit spread. Three vaccines were evaluated for efficacy chickens against a current North American clade 2.3.4.4b H5 HPAIV isolate, A/turkey/Indiana/3703-003/2022 H5N1. The included: 1) commercial inactivated reverse genetics (rg) generated H5N1 product with 2.3.4.4c hemagglutinin (HA) (rgH5N1); 2)...

Highly pathogenic avian influenza viruses (HPAIVs) of subtype H5 the Gs/GD/96 lineage remain a major threat to poultry due endemicity in wild birds. H5N1 HPAIVs from this were detected 2021 United States (U.S.) and since then have infected many domestic We evaluated pathobiology an early U.S. HPAIV (clade 2.3.4.4b, 2021) two H5N8 previous outbreaks 2.3.4.4c, 2014) Europe 2016) chickens turkeys. Differences clinical signs, mean death times (MDTs), virus transmissibility found between The bird...

Abstract Chickens, turkeys, ducks, quail and geese were challenged with SARS-CoV-2 or MERS-CoV. No disease was observed, no virus replication detected, antibodies not detected in serum. Neither replicated embryonating chicken’s eggs. Poultry are unlikely to serve a role the maintenance of either virus.

The outbreak of clade 2.3.4.4b H5 highly pathogenic avian influenza (HPAI) in North America that started 2021 has increased interest applying vaccination as a strategy to help control and prevent the disease poultry. Two commercially available vaccines based on recombinant herpes virus turkeys (rHVT) vector were tested against recent American HPAI isolate: A/turkey/Indiana/22-003707-003/2022 H5N1 specific pathogen free white leghorn (WL) chickens commercial broiler chickens. One rHVT-H5...

We report the results of a Brillouin scattering study elastic and photoelastic properties solid HF as function pressure up to about 120 kbar. performed near-forward-scattering experiments in which single-crystal samples lie plane bisecting angle. These measurements, made miniature Merrill-Bassett diamond-anvil cell, yield velocities longitudinal- transverse-acoustic phonons propagating along b axes single crystals orthorhombic HF. Relative magnitudes constants ${c}_{11}$, ${c}_{22}$,...

The incubation times for the eggs of 21 dinosaurs are determined from an estimate their embyronic metabolic rate and mass hatchlings via a growth model based on conservation energy. Embryos in extant birds crocodiles studied order to determine best embryonic metabolism growth. These results used develop theoretical that predicts egg. This is applied dinosaur provides unique window into reproduction. come both Saurischia Ornithischia. vary about 28 days Archaeopteryx lithographica 76...

Animal-vehicle collisions (AVCs) are a growing concern as the number of vehicle miles traveled and human encroachment into wildlife habitat increase throughout United States. Measures to prevent AVCs, such fencing passages, can impose significant investments for transportation agencies. An alternative potentially less expensive approach is use signs modify driver behavior reduce these collisions. This study investigated effectiveness seasonal animal movement advisories speed reduction tool...

Animal–vehicle collisions (AVCs) are a growing concern as the number of vehicle miles traveled and human encroachment into wildlife habitat increase throughout United States. Measures to prevent AVCs, such fencing passages, can impose significant investments for transportation agencies. An alternative potentially less expensive approach is use signs modify driver behavior reduce these collisions. This study investigated effectiveness seasonal animal movement advisories speed reduction tool...

Abstract Brillouin scattering has been used to study methanol as a function of pressure up 8.4 GPa. Our near-forward-scattering experiments yield the dependence velocity longitudinal acoustic (LA) phonons, while we determine product index refraction and LA phonon from our backscattering experiments. The lifetimes is obtained linewidth measured peaks. From find that normalized attenuation decreasing pressure.

A model based on cellular properties is used to analyze the mass growth curves of 20 dinosaurs. This analysis yields first measurement average metabolism The organismal also determined. dinosaurs found decrease with at a slower rate than observed in extant animals. increases dinosaur. These results come from both Saurischia and Ornithischia branches Dinosauria, suggesting that metabolic features were common all are compared data placental marsupial mammals, monotreme, altricial precocial...

Optoelectronic applications of Zn₃P₂ quantum dots have long been hindered by surface oxidation and defect states leading to low PL yield. The authors present shelling with ZnS prevent obtain luminescent particles.

We have measured the pressure dependence of both optical absorption and resonance Raman (RR) excitation profiles breathing mode, ${\ensuremath{\nu}}_{1}$, its first two overtones for substitutional impurity ion ${\mathrm{MnO}}_{4}^{\mathrm{\ensuremath{-}}}$, in crystalline ${\mathrm{KClO}}_{4}$. used powerful transform technique time-correlator theory RR scattering to analyze our data by calculating profile line shapes directly from absorption. This work constitutes successful application...

The ID-VD curves of the power transistor for boundary extraction electrical safe-operating area (E-SOA) are often measured by hundred-nanosecond pulse (TLP) to differentiate it from thermal SOA. However, E-SOA collapse at low gate voltage is still caused thermal. It Joule-heating lowering turn-on threshold bipolar as temperature increases. For high voltage, TLP induced magnetic-field transient, which leads current crowding effect well source-drain short effect. From electromagnetic...

Raman spectroscopy is used to probe the nature of hydrogen bonds which hold water hydration DNA. The ∼ 3450 cm(-1) molecular O-H stretching mode shows that first six molecules per base pair primary shell are very strongly bound observed shift in peak position this permits a determination length for these molecules. These appear be about 0.3 Å shorter than bulk water. linewidth no significant changes above contents 15 pair. This technique using vibrational obtain structural information shells...