A5072313773- Neurogenesis and neuroplasticity mechanisms
- Glycosylation and Glycoproteins Research
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
- Developmental Biology and Gene Regulation
- Mitochondrial Function and Pathology
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Axon Guidance and Neuronal Signaling
- Gastrointestinal disorders and treatments
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- Digestive system and related health
- Zebrafish Biomedical Research Applications
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Carbohydrate Chemistry and Synthesis
- Cytomegalovirus and herpesvirus research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Galectins and Cancer Biology
- Genetic Syndromes and Imprinting
Inserm
2002-2024
Université Paris Cité
2014-2024
Sorbonne Paris Cité
2014-2024
NeuroDiderot
2015-2024
Délégation Paris 7
2014-2020
Hôpital Robert-Debré
2011-2018
Infection, Anti-microbiens, Modélisation, Evolution
2015
Centre National de la Recherche Scientifique
1991-2014
Sorbonne Université
1999-2014
Centre de Génétique Moléculaire
2011
Neuropathological conditions might affect adult granulogenesis in the human dentate gyrus. However, radial glial cells (RGCs) have not been well characterized during development and aging. We previously described progenitor neuronal layer establishment hippocampal pyramidal gyrus from embryonic life until mid-gestation. Here, we describe RGC subtypes hippocampus 13 gestational weeks (GW) to mid-gestation characterize their evolution dynamics of neurogenesis adulthood normal Alzheimer's...
Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public health concern. The pathogenesis of cerebral lesions remains unclear. We report the neuropathologic substrates, immune response, and cellular targets CMV in 16 infected human fetal brains aged 23 to 28.5 gestational weeks. Nine cases were microcephalic, 10 had extensive cortical lesions, 8 hippocampal abnormalities, 5 showed olfactory bulb. density CMV-immunolabeled cells correlated presence...
Zinc fingers of the Cys2/His2 class are conserved 28 – 30 amino acid motifs that constitute an important and widespread family eukaryotic DNA-binding domains. It is therefore great interest to understand rules govern specific recognition DNA by zinc fingers. The domain transcription factor Krox–20 consists three fingers, each them making its primary contacts with a three-base pair subsite. We have performed data base-guided site-directed mutagenesis analysis Krox–20: nine derivatives were...
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
Highlights•MCPH1 expression is prominent in RGCs during the early stages of neocortical development•Early loss aRGCs due to Mcph1 mutation induces microcephaly mouse neocortex•Mcph1 influences cell proliferation and survival via crosstalk with metabolic pathways•Mcph1 stimulates mitochondrial activity glutaminolysis interaction VDAC1SummaryA distinctive feature development highly coordinated production different progenitor subtypes, which are critical for ensuring adequate neurogenic outcome...
Postmitotic neurons are produced from a pool of cycling progenitors in an orderly fashion that requires proper spatial and temporal coordination proliferation, fate determination, differentiation morphogenesis. This probably relies on complex interplay between mechanisms control cell cycle, specification differentiation. In this respect, we have studied the possible implication GATA2, transcription factor is involved several neuronal pathways, proliferation neural embryonic spinal cord....
Rabbit immunization with duodenal or colonic high molecular weight components allowed us to distinguish three new M3 antigens associated intestinal goblet cells; these were denoted as M3SI, M3D, and M3C. Using immunoperoxidase staining, the anti-M3SI serum labeled all cells in small intestine, a certain number of them colon, mainly its proximal part. The anti-M3D reacted primarily villosities, anti-M3C large, but not small, intestine. Moreover, M3C antigen was recovered some fetal duodenum,...
Abstract The identification of the factors that allow better monitoring stem cell renewal and differentiation is paramount importance for implementation new regenerative therapies, especially with regard to nervous hematopoietic systems. In this article, we present information on function zinc finger protein 191 (ZNF/Zfp191), a factor isolated in lines, within progenitors central system (CNS). ZNF/Zfp191 has been found be principally expressed developing CNS humans mice. Such an overlap...
Autosomal recessive primary microcephaly (MCPH) is a genetic disorder that causes reduction of cortical outgrowth without severe interference with patterning. It associated mutations in number genes encoding protein involved mitotic spindle formation and centrosomal activities or cell cycle control. We have shown previously blocking vasoactive intestinal peptide (VIP) during gestation mice by using VIP antagonist (VA) results microcephaly. Here, we the abnormalities caused prenatal VA...
The molecular mechanisms underlying the formation of hippocampus are unknown in humans. To improve our knowledge molecules that potentially regulate pyramidal neurogenesis and layering various hippocampal fields, we investigated expression progenitor markers cell fate from gestational week (GW) 9 to GW 20. At 9, compartment mainly consisted PAX6(+) cells ventricular zone. Between 11, a second germinal area, subventricular zone (SVZ), was formed, as shown by TBR2 labeling. Postmitotic (TBR1,...
Abstract Pituitary growth hormone (GH) and insulinlike factor (IGF)-1 are anabolic hormones whose physiological roles particularly important during development. The activity of the GH/IGF-1 axis is controlled by complex neuroendocrine systems including two hypothalamic neuropeptides, GH-releasing (GHRH) somatostatin (SRIF), a gastrointestinal hormone, ghrelin. neurotransmitter acetylcholine (ACh) involved in tuning GH secretion, its GH-stimulatory action has mainly been shown adults but not...
Motor activities are controlled by neural networks in the ventral spinal cord and consist motor neurons a set of distinct cardinal classes interneurons. These interneurons arise from progenitor domains (p0-p3) delineated according to transcriptional code. Neural progenitors each domain express unique combination transcription factors (TFs) that largely contribute determine fate four (V0-V3) neurons. In p2 domain, at least subtypes namely V2a, V2b, V2c, Pax6(+) V2 generated. Although genetic...
The molecular mechanisms that orchestrate the development of human dentate gyrus are not known. In this study, we characterized formation and fimbrial progenitors postmitotic neurons from 9 gestational weeks (GW9) to GW25. PAX6+ progenitor cells remained proliferative until GW16 in ventricular zone. By GW11, secondary matrix had developed intermediate zone, surrounding anlage streaming toward subpial layer. This contained proliferating and/or TBR2+ progenitors. parallel, SOX2+ were detected...
Changes in transcriptional regulation through cis-regulatory elements are thought to drive brain evolution. However, how this impacts the identity of primate cortical neurons is still unresolved. Here, we show that primate-specific sequences upstream Dbx1 gene promote human-like expression mouse embryonic cerebral cortex, and imparts cell identity. Indeed, while expressed highly restricted progenitors ventral pallium, it maintained primates. Phenocopy primate-like induces ectopic...