Exosomes are served as substitutes for stem cell therapy, playing important roles in mediating heart repair during myocardial infarction injury. Evidence have indicated that lipopolysaccharide (LPS) pre-conditioning bone marrow-derived mesenchymal cells (BMSCs) and their secreted exosomes promote macrophage polarization tissue several inflammation diseases; however, it has not been fully elucidated (MI). This study aimed to investigate whether LPS-primed BMSC-derived could mediate injury via...

Inosine 5'-monophosphate dehydrogenase type II (IMPDH2) was originally identified as an oncogene in several human cancers. However, the clinical significance and biological role of IMPDH2 remain poorly understood colorectal cancer (CRC).Quantitative real-time polymerase chain reaction (qPCR), western blotting analysis, Cancer Genome Atlas (TCGA) data mining immunohistochemistry were employed to examine expression CRC cell lines tissues. A series in-vivo in-vitro assays performed demonstrate...

// Na Chen 1, 2, * , Dan Guo 3, Qiong Xu 2 Minhui Yang Wang 1 Man Peng Yanqing Ding Shuang Jun Zhou Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China School Basic Sciences, 3 Pharmacy, These authors contributed equally to this work Correspondence to: Zhou, e-mail: jzhou16@163.com Wang, shuangw@126.com Keywords: FEZF1-AS1, FEZF1, colorectal cancer, long non-coding RNA Received: July 16, 2015      Accepted: January 21,...

Atherosclerosis is regarded as a chronic progressive inflammatory disease and basic pathophysiological process in coronary artery which life threatening clinic. The formation of foam cell plays key role the pathogenesis atherosclerosis. OxLDL significant factor progression disease. Our studies have demonstrated that USP14 promotes cancer development mediates cardiac hypertrophy LPS-induced inflammation. However, underlying mechanism unknown. In this study, we found inhibition significantly...

Abstract Acute carbon monoxide (CO) poisoning is a prevalent type of that causes significant harm globally. Delayed encephalopathy after acute (DEACMP) severe complication occurs CO poisoning; however, the exact underlying pathological cause DEACMP remains unclear. Accumulating evidence indicates abnormal inflammation and immune-mediated brain damage, cellular apoptosis autophagy, direct neuronal toxicity are involved in development delayed neurologic sequelae. Sodium butyrate, histone...

Abstract Atherosclerosis-associated cardiovascular diseases are main causes leading to high mortality worldwide. Macrophage-derived foam cell formation via uptaking modified lipoproteins is the initial and core step in process of atherosclerosis. Meanwhile, scavenger receptor indispensable for cells. UCHL1, a deubiquitinase, has been widely studied multiple cancers. UCHL1 could be an oncogene or tumor suppressor dependent types. It remains unknown whether influences cellular oxLDL uptake....

Myocardial ischemia/reperfusion (I/R) injury is a complex pathological process that still not fully understood. The oxidative stress response has critical role in the occurrence and progression of myocardial injury. This study investigated specific mechanism ubiquitin-specific protease 7 (USP7) regulation from perspective proteasome degradation its relation with Keap1 pathway, vital regulator cytoprotective responses to endogenous exogenous induced by reactive oxygen species (ROS)...

Mesoderm induction early response 1, family member 3 (MIER3) has recently been identified as a potential cancer susceptibility gene. However, the expression pattern and role of MIER3 in progression colorectal (CRC) have not yet well characterized. Here, we reported that was significantly reduced human primary associated with CRC metastasis poor prognosis. Moreover, up-regulation inhibited cell proliferation, migration invasion vitro repressed tumor growth vivo. In contrast, down-regulation...

Long non-coding RNAs (lncRNAs) have been demonstrated to serve important roles in the development and progression of cancer. Recently HNF1A antisense RNA 1 (HNF1A‑AS1), a lncRNA, has reported as exhibiting potential oncogenic role many types However, expression HNF1A‑AS1 colorectal carcinoma (CRC) remains unclear. In present study, CRC was examined for first time its correlation with cell biological behaviors analyzed. The results that distinctly upregulated tissues associated metastasis...

b-AP15 is a deubiquitinase (DUB) inhibitor of 19S proteasomes, which in turn targets ubiquitin C-terminal hydrolase 5 (UCHL5) and ubiquitin-specific peptidase 14 (USP14). Nuclear factor kappa B (NF-κB) closely linked to cellular response macrophages when the organism state microbial infection, it acts as vital part mechanism inflammatory reaction. However, molecular by DUB inhibitors, especially b-AP15, regulates inflammation remains poorly understood. This study aimed investigate...

Foam cell formation process is involved in the pathogenesis of atherosclerosis (AS). Activation this biological depends on lipid uptake by scavenger receptors, such as CD36, SR-A and SR-B1. Among these CD36 principal one because it dominates roughly 50% monocytes. In study, our western blotting RT-qPCR assays revealed that USP10 inhibition promotes degradation protein but does not change its mRNA level. addition, Co-IP results showed interacts with stabilizes cleaving poly-ubiquitin CD36....

The heart regeneration after apical resection and myocardial infarction in neonatal mice has been studied for years. However, the response of mouse under pressure overload is seldom explored. This study aimed to induce through a transverse aortic constriction (TAC) with different-gauge needles so as investigate effect on cardiomyocyte proliferation hypertrophy these mice. Myocardial was evaluated by echocardiographic, pathological, molecular analyses. Cardiomyocyte detected immune-staining...

The incidence and mortality of breast cancer (BCa) are the highest among female cancers.There approximate 70% BCa that classified as estrogen receptor alpha (ERα) positive.Therefore, targeting ERα is most significantly therapeutic schedule.However, patients with develop resistance to or (E2) antagonists such fulvestrant tamoxifen.In present study, we found L-Tetrahydropalmatine (L-THP) suppressed cell proliferation in + cells via inducing cycle arrest rather than apoptosis.Additionally,...

Abstract Cardiac fibrosis, featuring abnormally elevated extracellular matrix accumulation, decreases tissue compliance, impairs cardiac function and accelerates heart failure. Mounting evidence suggests that the ubiquitin proteasome pathway is involved in fibrosis. In present study, ubiquitin‐specific protease 2 (USP2) was identified as a novel therapeutic target Indeed, USP2 expression increased angiotensin II–induced primary fibroblasts (CFs) from neonatal rats. addition, inhibition...

The underlying mechanism of neointima formation remains unclear. Ubiquitin-specific peptidase 10 (USP10) is a deubiquitinase that plays major role in cancer development and progression. However, the function USP10 arterial restenosis unknown. Herein, expression was detected mouse arteries increased after carotid ligation. inhibition exhibited thinner model In vitro data showed deficiency reduced proliferation migration rat thoracic aorta smooth muscle cells (A7r5) human aortic (HASMCs)....

Allium macrostemon saponin is a traditional Chinese medicine that exhibits anti-atherosclerosis effects. However, the mechanism of its action has not been fully clarified. Platelet activation induced by CD40L plays an important role in process atherosis. In present study, we demonstrate for first time A. inhibits platelet CD40L. Moreover, effects on were achieved classical CD40L-associated pathway, including PI3K/Akt, MAPK and NF-κB proteins. addition, study further demonstrated exhibited...

Growth factor receptor bound protein 7 (GRB7) plays an important role in regulating the growth and metastasis of ovarian cancer. Angiogenesis is basis for growth, invasion, malignant tumors. In current study, we aimed to determine whether GRB7 a angiogenesis Immunohistochemistry on tissue microarray showed that platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) expression were positively correlated cancer tissues. knockdown suppressed vascular A (VEGFA) reduced VEGFA secretion. The...

Objective . To demonstrate various benign gynecologic diseases that can be performed by laparoendoscopic single‐site surgery (LESS) with conventional laparoscopic instruments. Method Patients need ovarian cystectomy, fallopian tube resection, or myomectomy were divided into experimental group and control group, perioperative outcomes of these patients analyzed. Results From November 2017 to May 2018, 65 LESS gynecological surgeries performed, among which there 25 cystectomies, 28 unilateral...

Abstract Ubiquitin-proteasome system (UPS) is involved in vascular smooth muscle cell (VSMC) proliferation. Deubiquitinating enzymes (DUBs) have an essential role the UPS-regulated stability of substrate; however, function DUBs intimal hyperplasia remains unclear. We screened to identify a protein responsible for regulating VSMC proliferation and identified USP14 that mediates cancer development, inflammation, foam formation. promotes human aortic A7r5 growth vitro, its inhibition or...