A5059431081- Extracellular vesicles in disease
- Lung Cancer Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- RNA modifications and cancer
- Medical Imaging and Pathology Studies
- Esophageal Cancer Research and Treatment
- MicroRNA in disease regulation
- Myasthenia Gravis and Thymoma
- Cancer Cells and Metastasis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Photoacoustic and Ultrasonic Imaging
- Advanced Radiotherapy Techniques
- Glycosylation and Glycoproteins Research
- Chronic Myeloid Leukemia Treatments
- Ultrasound and Hyperthermia Applications
- Nanowire Synthesis and Applications
- Icing and De-icing Technologies
- Anatomy and Medical Technology
- Intraoperative Neuromonitoring and Anesthetic Effects
- Ferroptosis and cancer prognosis
Korea University Medical Center
2015-2024
Korea University
2017-2024
Center for Art Studies
2021-2024
Guided Therapeutics (United States)
2024
Quality and Reliability (Greece)
2024
Lung cancer has a high mortality rate, but an early diagnosis can contribute to favorable prognosis. A liquid biopsy that captures and detects tumor-related biomarkers in body fluids great potential for early-stage diagnosis. Exosomes, nanosized extracellular vesicles found blood, have been proposed as promising biopsy. Here, we demonstrate accurate of lung cancer, using deep learning-based surface-enhanced Raman spectroscopy (SERS) the exosomes. Our approach was explore features cell...
Owing to the role of exosome as a cargo for intercellular communication, especially in cancer metastasis, evidence has been consistently accumulated that exosomes can be used noninvasive indicator cancer. Consequently, several studies applying have proposed diagnostic methods such ELISA assay. However, it still challenging get reliable results due requirement labeling process and high concentration exosome. Here, we demonstrate label-free highly sensitive classification method by combining...
Abstract Early cancer detection has significant clinical value, but there remains no single method that can comprehensively identify multiple types of early-stage cancer. Here, we report the diagnostic accuracy simultaneous 6 cancers (lung, breast, colon, liver, pancreas, and stomach) by analyzing surface-enhanced Raman spectroscopy profiles exosomes using artificial intelligence in a retrospective study design. It includes classification models recognize signal patterns plasma to both their...
Protein mutation detection using liquid biopsy can be simply performed periodically, making it easy to detect the occurrence of newly emerging mutations rapidly. However, has low diagnostic accuracy since there are more normal proteins than mutated in body fluids. To increase accuracy, we analyzed plasma exosomes nanoplasmonic spectra and deep learning. Exosomes, a promising biomarker, abundant stably carry intact originating from mother cells. exosomal cannot detected sensitively because...
Near-infrared (NIR) fluorescence imaging provides surgeons with real-time visual information during surgery. The purpose of this pilot trial was to evaluate the safety and feasibility intraoperative detection pulmonary neoplasms NIR after low-dose indocyanine green (ICG) injection.Eleven consecutive patients who were scheduled undergo resection enrolled in study. ICG (1 mg/kg) administered intravenously 1 day before surgery, retrieved surgical specimens examined for signalling by using...
Identification of the tumor margin during surgery is important for precise minimal resection lung tumors. Intravenous injection indocyanine green (ICG) has several limitations when used intraoperative visualization cancer.To describe a technique using ICG inhalation and evaluate clinical applicability in mouse rabbit models as well specimens patients with tumors.In both mice rabbits, distribution inhaled was investigated vivo ex near-infrared imaging system. Lung detection via evaluated by...
This study was conducted to develop a fluorescent iodized emulsion comprising indocyanine green (ICG) solution and lipiodol (ethiodized oil) evaluate its feasibility for use in clinical setting.ICG the preoperative localization of pulmonary nodules is limited terms penetration depth diffusion.First, microscopy used investigate distribution ICG-lipiodol emulsions prepared using different methods. The were injected 15 lung lobes 3 rabbits under computed tomography fluoroscopy guidance;...
Extracellular vesicles (EVs) have garnered significant attention due to their potential applications in disease diagnostics and management. However, the process of isolating EVs, primarily from blood samples, is still suboptimal. This mainly attributed abundant nature soluble proteins lipoproteins, which are often separated together with EVs end products conventional isolation methods. As such, we devise a single-step charge-based EV method by utilizing positively charged beads selectively...
Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of BCR-ABL transcript. This study aimed to identify these possibilities.Human CML cell line-derived exosomes CML-patients-derived were isolated with a size-exclusion chromatography column ExoQuick™ exosome precipitation solution, respectively. Isolated analysed by nested PCR detect transcript.Exosomes two human lines yielded 250-bp band. RNA sequence analysis revealed...
No specific markers have been identified to detect non-small cell lung cancer (NSCLC) cell-derived exosomes circulating in the blood. Here, we report a new biomarker that distinguishes between and non-cancer exosomes. Exosomes isolated from patient plasmas at various pathological stages of NSCLC, NSCLC lines, human pulmonary alveolar epithelial cells using size exclusion chromatography were characterized. The GRIP coiled-coil domain-containing 2 (GCC2) protein, involved endosome-to-Golgi...
Segmentectomy is a targeted surgical approach tailored for patients with compromised health and early-stage lung cancer. The key to successful segmentectomy lies in precisely identifying the tumor intersegmental planes ensure adequate resection margins. In this study, we aimed enhance process by simultaneously visualizing through intravenous injection of indocyanine green (ICG) at different time points doses. Lung tumors were detected ICG dose 2 mg/kg 12 h before surgery rabbit model....
We report the development of an injectable hydrogel-based dual computed tomography (CT) and near-infrared (NIR) imaging marker for use in preoperative pulmonary localization intraoperative dual-imaging deep-seated nodules.
The technique of simultaneously visualizing pulmonary nodules and the intersegmental plane using fluorescent images was developed to measure distance between them intraoperatively.Patients who underwent segmentectomy were consecutively included in this study March 2016 July 2019. Computed tomography or electromagnetic bronchoscopy-guided localization with indocyanine green-lipiodol emulsion performed on day surgery. In middle surgery, after dividing segmental artery, vein bronchus a targeted...
Abstract Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field EV research. The presence lipoproteins and soluble proteins often hinders isolation high purity EVs upon utilization conventional separation methods. To circumvent such problems, we designed single-step dual size-exclusion chromatography (dSEC) column for effective highly bone marrow derived human plasma. With an aim to select appropriate design parameters, analyzed...
Extracellular vesicle (EV) proteins from acute myeloid leukemia (AML) cell lines were analyzed using mass spectrometry. The analyses identified 2450 proteins, including 461 differentially expressed (290 upregulated and 171 downregulated). CD53 CD47 selected as candidate biomarkers. association between survival of patients with AML the expression levels at diagnosis was mRNA data Cancer Genome Atlas database. Patients higher showed significantly inferior than those lower levels. ELISA results...
Preclinical studies of lung cancer require suitable large-animal models to allow evaluation and development surgical interventional techniques. We assessed the feasibility safety a novel rabbit model solitary tumors, in which real-time computed tomography fluoroscopy is used guide inoculation VX2 carcinoma single-cell suspensions. Thirty-eight rabbits were divided into four groups according volume tissue or cell suspension, lipiodol, Matrigel, injection needle size. The mixtures...
Segmentectomy, recommended for early-stage lung cancer or compromised function, demands precise tumor detection and intersegmental plane identification. While indocyanine green (ICG) commonly aids in these aspects using near-infrared imaging, its separate administrations through different routes times can lead to complications patient anxiety. This study aims develop a lung-specific delivery method by nebulizing low-dose ICG targeted segments, allowing simultaneous of tumors planes across...
Indocyanine green (ICG) has been used to detect several types of tumors; however, its ability metastatic lymph nodes (LNs) remains unclear. Our goal was determine the feasibility ICG in detecting LNs. We established a mouse model and evaluated potential ICG. The LNs also patients with lung or esophageal cancer, detected computed tomography (CT) positron-emission (PET)/CT, scheduled undergo surgical resection. Tumors were successfully mice. In clinical study, efficacy 15 tumors fifty-four...
The interface trap density in single-walled carbon nanotube (SWNT) network thin-film transistors (TFTs) is a fundamental and important parameter for assessing the electronic performance of TFTs. However, number studies on extraction densities, particularly SWNT TFTs, has been insufficient. In this work, we propose an efficient technique extracting energy-dependent traps From measured dispersive, frequency-dependent capacitance-voltage (C-V) characteristics, dispersive-free,...
With advances in surgical technology, thoracic surgeons have widely adopted minimally invasive limited-resection techniques to preserve normal tissues. However, it remains difficult achieve situ localization of invisible pulmonary nodules during surgery. Therefore, we proposed an ultra-low-dose X-ray imaging device for intraoperative nodule surgeries.The features a hand-held type and consists carbon nanotube-based source intraoral dental sensor. In preclinical study, created pseudo using ex...
The different pathways between the position of a near-infrared camera and user’s eye limit use existing fluorescence imaging systems for tumor margin assessments. By utilizing an optical system that precisely matches image path visible light, we developed augmented reality (AR)-based provides users with real-field, without requiring any additional algorithms. Commercial smart glasses, dichroic beam splitters, mirrors, custom cameras were employed to develop proposed system, each mount was...
Jiyun Rho, Yu Hua Quan, Byeong Hyeon Choi, Kook Nam Han, Beop-Min Kim, Young Ho Hyun Koo Kim