A5052668734- Malaria Research and Control
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Computational Drug Discovery Methods
- Microbial infections and disease research
- Drug-Induced Hepatotoxicity and Protection
- HIV/AIDS drug development and treatment
- Drug Transport and Resistance Mechanisms
- Milk Quality and Mastitis in Dairy Cows
- Research on Leishmaniasis Studies
- Probiotics and Fermented Foods
- Complement system in diseases
- Traditional and Medicinal Uses of Annonaceae
- Parasites and Host Interactions
- Endoplasmic Reticulum Stress and Disease
- Neurological diseases and metabolism
- Venomous Animal Envenomation and Studies
- Primate Behavior and Ecology
- Vector-Borne Animal Diseases
- Berberine and alkaloids research
- Bioactive Compounds and Antitumor Agents
- Pharmacological Effects of Natural Compounds
- Bacterial Identification and Susceptibility Testing
- Rabies epidemiology and control
- Phytochemistry and biological activity of medicinal plants
Jomo Kenyatta University of Agriculture and Technology
2016-2025
National Institute of Allergy and Infectious Diseases
2025
Kenya Medical Research Institute
2011-2022
University of Ghana
2017-2018
Abstract Strengthening malaria surveillance is a key intervention needed to reduce the global disease burden. Reliable serological markers of recent exposure could improve current methods by allowing for accurate estimates infection incidence from limited data. We studied IgG antibody response 111 Plasmodium falciparum proteins in 65 adult travellers followed longitudinally after natural complete absence re-exposure. identified combination five that detect within previous three months with...
ABSTRACT Artemisinin-based combination therapies (ACTs) are vital for malaria treatment, but these threatened by blood-stage persisters—dormant forms of Plasmodium parasites that can survive drug exposure and cause recrudescent infections. Here, we present improved protocols efficient preparation pure falciparum persister populations without the need magnetically activated columns, sorbitol exposure, or prolonged manipulations. Our transformed actively replicating into exposing mixed to...
ABSTRACT Kinship is a major determinant of affiliative and mating behavior in primates. In field studies, identifying kin typically relies part on genetic analysis, especially for discriminating paternal relationships. Such analyses assume knowledge Mendelian inheritance, genotyping technologies, basic statistical inference. Consequently, they can be difficult students to grasp, particularly through traditional lecture formats. Here, we investigate whether integrating an additional active...
<title>Abstract</title> <bold>Background:</bold> The discovery of <italic>Microsporidia MB</italic>, a malaria-impairing symbiont in <italic>Anopheles arabiensis</italic>, suggests its potential for malaria control. This study investigated prevalence and diversity <italic>An. gambiae</italic> s.l. funestus</italic> Busia, Kenya. also explored association with insecticide resistance environmental factors. <bold>Methods:</bold> Mosquito larvae adults were collected from three sub-counties...
We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia 90% (ED(90)) of PQ LM against the parent line were 3.52 3.93 mg/kg, respectively. After pressure (more than 27 passages), had resistance indexes (I(90)) [ED(90) line/ED(90) line] 68.86 63.55, growing them absence for 10 passages cryo-preserving at -80 degrees C least 2 months, phenotypes remained stable. Cross-resistance...
Retroviral protease inhibitors (RPIs) such as lopinavir (LP) and saquinavir (SQ) are active against Plasmodium parasites. However, the exact molecular target(s) for these RPIs in parasites remains poorly understood. We hypothesised that LP SQ suppress parasite growth through inhibition of aspartyl proteases. Using reverse genetics approach, we embarked on separately generating knockout (KO) lines lacking Plasmepsin 4 (PM4), PM7, PM8, or DNA damage-inducible protein 1 (Ddi1) rodent malaria...
Abstract Background Literature is scarce on the occurrence of bovine mastitis and antimicrobial resistance among dairy animals kept by pastoralists in Kenya. Objectives A cross‐sectional study was carried out to investigate prevalence risk factors subclinical (SCM) evaluate antibiotic sensitivity bacteria isolated from cattle farmers Kajiado Central sub‐county, Methods total 202 lactating cows 40 farms were sampled. Milk screened for SCM using California test, present milk samples determined...
The evolution of drug-resistant parasites is a major hindrance to malaria control, and thus understanding the behaviour mutants clinical relevance. study aimed investigate how resistance against lumefantrine (LU) piperaquine (PQ), anti-malarials used as partner drugs in artemisinin-based combination therapy (ACT), impacts parasite fitness. This important since ACT, first-line anti-malarial regimen increasingly being reported.The stability Plasmodium berghei ANKA strain that was previously...
The Plasmodium falciparum Circumsporozoite Protein (PfCSP) has been used in developing the RTS,S, and R21 malaria vaccines. However, genetic polymorphisms within Pfcsp compromise effectiveness of vaccine. Thus, it is essential to continuously assess diversity , especially when deploying across different geographical regions. In this study, we assessed on isolates from Homabay County, a malaria-endemic region western Kenya, compared against other Kenya. We extracted DNA 27 microscopically...
<ns4:p><ns4:bold>Background:</ns4:bold> The human malaria parasite <ns4:italic>Plasmodium falciparum</ns4:italic> has evolved drug evasion mechanisms to all available antimalarials. combination of amodiaquine-artesunate is among the choice for treatment uncomplicated malaria. In this combination, a short-acting, artesunate partnered with long-acting, amodiaquine which resistance may emerge rapidly especially in high transmission settings. Here, we used rodent berghei </ns4:italic>ANKA as...
Aim: This study determined the resistance pattern to β-lactam antibiotics of bacteria isolated from goats with subclinical mastitis in Thika subcounty, Kenya. We also administered a questionnaire assess risk factors associated occurrence commonly used antibiotics. Materials and Methods: collected milk samples 110 lactating dairy subcounty screen for using California test. Bacterial isolation identification were performed according colony morphology, hemolytic on sheep blood agar, lactose...
Introduction: Chemotherapy still is the most effective way to control malaria, a major public health problem in sub-Saharan Africa. The large-scale use of combination therapy artemether-lumefantrine for malaria treatment Africa predisposes lumefantrine emergence resistance. There need identify drugs that can be used as substitutes therapy. Methylene blue, synthetic anti-methemoglobinemia drug, has been shown contain antimalarial properties, making it candidate drug repurposing. present study...
Reduced sensitivity of the human malaria parasite, Plasmodium falciparum, to Artemisinin and its derivatives (ARTs) threatens global efforts towards eliminating malaria. ARTs have been shown cause ubiquitous cellular genetic insults, which results in activation unfolded protein response (UPR) pathways. The UPR restores homeostasis, otherwise would be toxic survival. Here, we interrogated role DNA-damage inducible 1 (PfDdi1), a unique proteasome-interacting retropepsin mediating actions ARTs....
<ns4:p><ns4:bold>Background:</ns4:bold> The human malaria parasite <ns4:italic>Plasmodium falciparum</ns4:italic> has evolved complex drug evasion mechanisms to all available antimalarials. To date, the combination of amodiaquine-artesunate is among choice for treatment uncomplicated malaria. In this combination, a short acting, artesunate partnered with long amodiaquine which resistance may emerge rapidly especially in high transmission settings. Here, we used rodent berghei...
Cancer is a major public health burden in both developed and developing countries.The current conventional cancer therapies like chemotherapy are expensive inaccessible to many patients.Commercial wild edible mushrooms becoming more important for their nutritional value an alternative source of immune modulation anticancer agents.Although Previous studies with Trametes versicolor mushroom from various parts the world have demonstrated antiproliferative activity on cell lines, recently...
Strengthening malaria surveillance is a key intervention needed to reduce the global disease burden. Reliable serological markers of recent exposure could dramatically improve current methods by allowing for accurate estimates infection incidence from limited data. We studied IgG antibody response 111 Plasmodium falciparum proteins in travellers followed longitudinally after natural complete absence re-exposure. identified novel combination five (GAMA, MSP1, MSPDBL1 C- and N-terminal,...
Antimicrobial resistance (AMR) is a growing health problem globally. To address this challenge, there need to generate baseline data on the prevalence and AMR profile of main disease-causing bacteria. Here, we interrogated bacteria in nasal cavity healthy pastoralists Kajiado Central Subcounty, Kenya, occurrence Staphylococcus isolates among study subjects. Nasal swabs from 176 were cultured, identified using standard phenotypic biochemical bacteriological methods. Among obtained 195...